Molecular Analysis of Curcumin-induced Polarization of Murine RAW264.7 Macrophages

Chen, Fangyuan; Guo, Ning; Cao, Guofen; Zhou, Juan; Yuan, Zuyi

doi:10.1097/fjc.0000000000000079

Cited by 49 publications

(26 citation statements)

References 47 publications

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“…Yin et al showed that curcumin inhibited caspase-1 activation and IL-1β secretion through suppressing lipopolysaccharide (LPS) priming and NLRP3 inflammasome in mouse bone marrow-derived macrophages, and confirmed these results in vivo in a model of high-fat diet-induced insulin resistance in wild-type C57BL/6 mice [72]. Interestingly, in an in vitro study, Chen et al demonstrated that curcumin inhibits the M1 inflammatory phenotype of RAW264.7 macrophages as a result of the direct activation of the inhibitor protein κB-α (IkB-α) and polarizes the macrophages to become anti-inflammatory M2 phenotype through the activation of PPARγ [73]. Later on, the same group reported that curcumin dramatically reduced oxLDL-induced IL-1β, IL-6 and TNF-α cytokine production in M1 derived from M0 RAW264.7 macrophages [74].…”

Section: Introductionmentioning

confidence: 70%

Phenolic Compounds Exerting Lipid-Regulatory, Anti-Inflammatory and Epigenetic Effects as Complementary Treatments in Cardiovascular Diseases

et al. 2020

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Atherosclerosis is the main process behind cardiovascular diseases (CVD), maladies which continue to be responsible for up to 70% of death worldwide. Despite the ongoing development of new and potent drugs, their incomplete efficacy, partial intolerance and numerous side effects make the search for new alternatives worthwhile. The focus of the scientific world turned to the potential of natural active compounds to prevent and treat CVD. Essential for effective prevention or treatment based on phytochemicals is to know their mechanisms of action according to their bioavailability and dosage. The present review is focused on the latest data about phenolic compounds and aims to collect and correlate the reliable existing knowledge concerning their molecular mechanisms of action to counteract important risk factors that contribute to the initiation and development of atherosclerosis: dyslipidemia, and oxidative and inflammatory-stress. The selection of phenolic compounds was made to prove their multiple benefic effects and endorse them as CVD remedies, complementary to allopathic drugs. The review also highlights some aspects that still need clear scientific explanations and draws up some new molecular approaches to validate phenolic compounds for CVD complementary therapy in the near future.In the last decade the scientific researchers turned their attention to phytochemicals, as effective, safe and low-cost natural bioactive compounds for CVD treatment.Dyslipidemia consists of increased blood concentrations of total cholesterol (TC), low density lipoproteins-cholesterol (LDL-C) and/or triglycerides (TG), and decreased high density lipoproteins-cholesterol (HDL-C) [6]. The lipid metabolism is complex and the candidate mechanisms that could generate dyslipidemia include: (i) excessive dietary lipid absorption in the small intestine; (ii) packing of exogenous lipids with cholesterol and fatty acids produced de novo in the liver and their secretion as very low density lipoproteins (VLDL); (iii) hydrolysis of TG from VLDL by lipases and their conversion into LDL, which are taken up by the peripheral tissues through LDL receptor (LDL-R) and scavenger receptors; (iv) diminished production of HDL by the liver and small intestine, thereby decreasing reverse cholesterol transport (RCT) from the peripheral tissues to the liver; (v) lowered excess cholesterol excretion from the liver into gallbladder or to the intestinal lumen through the ATP-binding cassette G5 and G8 transporters (ABCG5/G8) that facilitate trans-intestinal cholesterol efflux (TICE). Dyslipidemia is associated with the accumulation of LDL in the sub-endothelium of the artery wall. At this site, LDL undergoes oxidative modifications (oxLDL) that trigger inflammatory responses, and is taken up by the monocyte-derived macrophages infiltrated in the sub-endothelium which thus become lipid-loaded foam cells, the hallmark of atheroma development [7]. Until now, the most effective lipid-lowering treatment for hyperlipidemic patients was the statin therapy. But recen...

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Section: Introductionmentioning

confidence: 70%

Phenolic Compounds Exerting Lipid-Regulatory, Anti-Inflammatory and Epigenetic Effects as Complementary Treatments in Cardiovascular Diseases

et al. 2020

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“…However, other studies have indicated that oral administration of a curcumin solution (3000 mg/kg) significantly reduces tumor neocapillary density and serum VEGF levels in mice with HepG2 hepatocellular carcinoma [62]. Furthermore, although treatment of RAW264.7 macrophages with 1 mM of hydrazinocurcumin encapsulated nanoparticles induced polarization of macrophages from M2 to M1 phenotype through inhibition of STAT3 [63], 25 μmol/L of curcumin, on the contrary, stimulated polarization of these cells into M2 phenotype via activation of proliferator-activated receptor gamma and secretion of IL-4 or IL-13 [64. 65].…”

Section: Natural Anti-angiogenic Compounds Derived From Plantsmentioning

confidence: 99%

Plants and their active compounds: natural molecules to target angiogenesis

Bhat

Basu

2016

Angiogenesis

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Angiogenesis, or new blood vessel formation, is an important process in the pathogenesis of several diseases and thus has been targeted for the prevention and treatment of many disorders. However, the anti-angiogenic agents that are currently in use are mainly synthetic compounds and humanized monoclonal antibodies, which are either expensive or toxic, thereby limiting their use in many patients. Therefore, it is necessary to identify less toxic, inexpensive, novel and effective anti-angiogenic molecules. Several studies have indicated that natural plant products can meet these criteria. In this review, we discuss the anti-angiogenic properties of natural compounds isolated from plants and the molecular mechanisms by which these molecules act. Finally, we summarize the advantages of using plant products as anti-angiogenic agents. Compared with currently available anti-angiogenic drugs, plant products may not only have similar therapeutic potential but are also inexpensive, less toxic and easy to administer. However, novel and effective strategies are necessary to improve their bioavailability for clinical use.

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“…Previous findings indicated that curcumin be able to not only directly stimulate the polarization of macrophages to M2 phenotype, but also induce switching of macrophages from M1 to M2 phenotype [7][8][9]. Curcumin, as a major bioactive constituent in turmeric (Curcuma longa), has been described as a brilliant molecule among lots of naturally occurring substances for powerful anti-inflammatory, antioxidant, antidiabetic and anticancer activities [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Macrophage repolarization using CD44-targeting hyaluronic acid–polylactide nanoparticles containing curcumin

Farajzadeh

Zarghami

Serati-Nouri

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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The aim of this study was to evaluate the efficiency of using a natural substance, curcumin, encapsulated in CD44-targeting hyaluronate-polylactide (HA-PLA) nanoparticles (NPs) for the modulation of macrophage polarity from the pro-inflammatory M1 to anti-inflammatory M2 phenotype. For this purpose, the characterization of the NPs was monitored using HNMR, FTIR, DLS and FE-SEM. The effects of curcumin-encapsulated HA-PLA NPs on the viability of LPS/IFN-γ stimulated peritoneal macrophages were determined using MTT assay. The cellular uptake of free curcumin and nano-formulated curcumin was assessed using confocal microscopy. Also, the expression levels of iNOS-2 (M1 marker), Arg-1 (M2 marker) and also pro-inflammatory cytokines were measured by real-time PCR. Data showed that the nano-formulated curcumin with spherical shape, an average diameter of 102.5 nm and high cellular uptake was significantly less toxic to peritoneal macrophages. Furthermore, the nano-formulated curcumin effectively indicated a reduction in iNOS-2 and an increase in Arg-1 levels than free curcumin. The change in macrophage phenotype by curcumin-encapsulated HA-PLA NPs could suppress the inflammation in LPS/IFN-γ stimulated macrophages as evidenced by a major reduction in pro-inflammatory cytokines. Conclusively, the results suggested that the curcumin formulation with CD44-targeting HA-PLA NPs might be a promising platform for the treatment of inflammatory diseases.

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Molecular Analysis of Curcumin-induced Polarization of Murine RAW264.7 Macrophages

Cited by 49 publications

References 47 publications

Phenolic Compounds Exerting Lipid-Regulatory, Anti-Inflammatory and Epigenetic Effects as Complementary Treatments in Cardiovascular Diseases

Phenolic Compounds Exerting Lipid-Regulatory, Anti-Inflammatory and Epigenetic Effects as Complementary Treatments in Cardiovascular Diseases

Plants and their active compounds: natural molecules to target angiogenesis

Macrophage repolarization using CD44-targeting hyaluronic acid–polylactide nanoparticles containing curcumin

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