Molecular and Biological Aspects of the Bovine Immunodeficiency Virus

Corredor, Andrea; St-Louis, Marie-Claude; Archambault, Denis

doi:10.2174/157016210790416343

Cited by 12 publications

(23 citation statements)

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“…Lentiviruses infect a broad array of mammalian species, including humans and simian, bovine, equine, caprine, and ovine species (12). The outcome of lentivirus-associated infections ranges from benign and subclinical (as for BIV) to severe debilitating and lethal disease (as for HIV-1) (12).…”

Section: Discussionmentioning

confidence: 99%

“…The outcome of lentivirus-associated infections ranges from benign and subclinical (as for BIV) to severe debilitating and lethal disease (as for HIV-1) (12). Cell tropism, protective host cellular factors, and a potential hiding site from the host's immune system have all been considered to explain differences in pathogenicity among lentiviruses (50).…”

Section: Discussionmentioning

confidence: 99%

“…The leucinerich NESs were first identified in the human immunodeficiency virus type 1 (HIV-1) Rev protein (16) and the cyclic AMP (cAMP)-dependent protein kinase inhibitor (PKI) (71) with the consensus sequence motif ⌽-X 2-3 -⌽-X 2-3 -⌽-X-⌽ (where ⌽ is L, I, V, F, or M and X refers to any amino acid residue) (41). Similarly to the importins (17, 56, 64), the CRM1 export process depends on the Ran GTP-GDP cycle and the interaction between a NES and CRM1 can be inhibited by the, antifungal antibiotic leptomycin B (17, 40).Bovine immunodeficiency virus (BIV) is a lentivirus of the Retroviridae family that shares morphological, genetic, antigenic, and/or biological properties with HIV-1 and other animal lentiviruses, including the equine infectious anemia virus (EIAV) (12,22). The BIV provirus DNA of 8.960 kb in length has a typical retroviral genomic structure containing the gag, pol, and env genes flanked by long terminal repeats (LTRs) at the 5= and 3= termini (12,23).…”

mentioning

confidence: 99%

“…Bovine immunodeficiency virus (BIV) is a lentivirus of the Retroviridae family that shares morphological, genetic, antigenic, and/or biological properties with HIV-1 and other animal lentiviruses, including the equine infectious anemia virus (EIAV) (12,22). The BIV provirus DNA of 8.960 kb in length has a typical retroviral genomic structure containing the gag, pol, and env genes flanked by long terminal repeats (LTRs) at the 5= and 3= termini (12,23).…”

mentioning

confidence: 99%

“…The BIV provirus DNA of 8.960 kb in length has a typical retroviral genomic structure containing the gag, pol, and env genes flanked by long terminal repeats (LTRs) at the 5= and 3= termini (12,23). In proximity to the pol-env junction, the BIV genome contains additional open reading frames (ORFs) that may encode nonstructural regulatory/accessory proteins such as the Rev protein (12,23).…”

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confidence: 99%

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The Bovine Immunodeficiency Virus Rev Protein: Identification of a Novel Nuclear Import Pathway and Nuclear Export Signal among Retroviral Rev/Rev-Like Proteins

Corredor

Archambault

2012

J Virol

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The Rev protein is essential for the replication of lentiviruses. Rev is a shuttling protein that transports unspliced and partially spliced lentiviral RNAs from the nucleus to the cytoplasm via the nucleopore. To transport these RNAs, the human immunodeficiency virus type 1 (HIV-1) Rev uses the karyopherin β family importin β and CRM1 proteins that interact with the Rev nuclear localization signal (NLS) and nuclear exportation signal (NES), respectively. Recently, we reported the presence of new types of bipartite NLS and nucleolar localization signal (NoLS) in the bovine immunodeficiency virus (BIV) Rev protein. Here we report the characterization of the nuclear import and export pathways of BIV Rev. By using an in vitro nuclear import assay, we showed that BIV Rev is transported into the nucleus by a cytosolic and energy-dependent importin α/β classical pathway. Results from glutathione S -transferase (GST) pulldown assays that showed the binding of BIV Rev with importins α3 and α5 were in agreement with those from the nuclear import assay. We also identified a leptomycin B-sensitive NES in BIV Rev, which indicates that the protein is exported via CRM1 like HIV-1 Rev. Mutagenesis experiments showed that the BIV Rev NES maps between amino acids 109 to 121 of the protein. Remarkably, the BIV Rev NES was found to be of the cyclic AMP (cAMP)-dependent protein kinase inhibitor (PKI) type instead of the HIV-1 Rev type. In summary, our data showed that the nuclear import mechanism of BIV Rev is novel among Rev proteins characterized so far in lentiviruses.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The Bovine Immunodeficiency Virus Rev Protein: Identification of a Novel Nuclear Import Pathway and Nuclear Export Signal among Retroviral Rev/Rev-Like Proteins

Corredor

Archambault

2012

J Virol

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Molecular detection of bovine immunodeficiency virus in water buffaloes (Bubalus bubalis) from the Amazon region, Brazil

Albernaz

Leite

Reis

et al. 2015

Trop Anim Health Prod

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Bovine immunodeficiency is a chronic progressive disease caused by a lentivirus that affects cattle and buffaloes. Although the infection has been described in cattle in some countries, including in Brazil, there are only two reports of infection in buffaloes: one in Pakistan and one in Cambodia. The aim of the present study was to survey the occurrence of bovine immunodeficiency virus (BIV) in water buffaloes from the Amazon region, Pará state, Brazil. BIV proviral DNA was surveyed in 607 whole blood samples of water buffaloes from 10 farms located in the state of Pará using semi-nested polymerase chain reaction (PCR) (PCR-SN) to amplify the pol region of the viral genome. Of the 607 samples tested, 27 (4.4 %) were positive for BIV proviral DNA. The amplified fragments were confirmed by sequence analysis after cloning and nucleotide sequencing. The sequence obtained had 99 % similarity to the reference strain (R-29). The present study provides important epidemiological data because BIV was detected for the first time in water buffaloes in Brazil. Further, the results suggest the possibility of the virus being a risk factor for herd health because it may be a potential causal agent of chronic disease and, also may be associated to other infectious diseases.

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Animal Lentiviruses

Stover

Watson

2015

Health of HIV Infected People

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Molecular and Biological Aspects of the Bovine Immunodeficiency Virus

Cited by 12 publications

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The Bovine Immunodeficiency Virus Rev Protein: Identification of a Novel Nuclear Import Pathway and Nuclear Export Signal among Retroviral Rev/Rev-Like Proteins

The Bovine Immunodeficiency Virus Rev Protein: Identification of a Novel Nuclear Import Pathway and Nuclear Export Signal among Retroviral Rev/Rev-Like Proteins

Molecular detection of bovine immunodeficiency virus in water buffaloes (Bubalus bubalis) from the Amazon region, Brazil

Animal Lentiviruses

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