Molecular and cellular biomarker responses to pesticide exposure in juvenile chinook salmon (Oncorhynchus tshawytscha)

Eder, Kai J.; Leutenegger, Christian M.; Wilson, Barry W.; Werner, Ingeborg

doi:10.1016/j.marenvres.2004.03.098

Cited by 35 publications

(42 citation statements)

References 8 publications

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“…Such pollutants can induce various biological responses in fish, affecting the organisms from the biochemical to the population-community levels (Hugla et al, 1995;Kovacs et al, 2002;Porter and Janz, 2003;Eder et al, 2004). During the last twenty years, a large body of biomonitoring data has been accumulated in order to assess whether lower level responses could be used as indicators to determine toxic effect of xenobiotics .…”

Section: Introductionmentioning

confidence: 99%

Multiscale approach of fish responses to different types of environmental contaminations: A case study

Mayon

Bertrand

Leroy

et al. 2006

Science of The Total Environment

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In order to study the responses of wild fish to environmental contaminations, different sites (two references and three contaminated) were sampled across the Walloon hydrographical network (southern Belgium). The status of fish communities was characterized according to an index of biotic integrity (IBI). Furthermore, population structure, reproductive parameters and biochemical assays were performed on chub (Leuciscus cephalus) as sentinel species. The results showed impaired responses in fish sampled downstream paper mill effluents (BKME), in a polychlorinated biphenyls (PCB) contaminated site, and in a river receiving pesticides (i.e. atrazine and hexachlorocyclohexane). High level of hepatic 7-ethoxyresorufin-Odeethylase (EROD) activity were evidence of direct toxicant exposure, while population structures showed unbalanced size-frequency distributions. Moreover, in the pesticide contaminated site, a higher proportion of atretic oocytes was found in females and abnormally high plasma alkali-labile phosphorus (ALP) values were noticed in males, compared to reference sites, suggesting that chubs were exposed to estrogenic compounds. IBI scores from both sites were also lower than in reference sites. On the other hand, no particular response was found in hepatic glutathione-S-transferase (GST) activities, hepatic heat-shock protein HSP70 concentrations and plasma sex steroids (estradiol-17β, 11-ketotestosterone and testosterone) in chubs from any sites. By using a multiparametric approach, a better understanding of response mechanisms to pollution in fish may be achieved in order to improve the ecological status of river ecosystems.

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Section: Introductionmentioning

confidence: 99%

Multiscale approach of fish responses to different types of environmental contaminations: A case study

Mayon

Bertrand

Leroy

et al. 2006

Science of The Total Environment

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“…Cytokine production is considered as a sensitive indicator for monitoring perturbations of the immune system caused by xenobiotics and could be a useful biomarker for environmental pollutant exposure (Hooghe et al 2000;Jeon et al 2001;Eder et al 2004;Lee et al 2010). Although published data show that IFN-γ (type 1 cytokine) is not necessary for neopterin production (Sghiri et al 2005), it is the most potent inducer of neopterin production.…”

Section: Discussionmentioning

confidence: 99%

Effect of prochloraz fungicide on neopterin and biopterin concentrations in blood plasma of common carp

et al. 2014

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Neopterin and biopterin are often used as markers of cell mediated immunity. The aim of this study was to evaluate the effect of prochloraz on neopterin and biopterin concentrations in blood plasma of common carp as possible indicators for monitoring perturbations of the immune system caused by xenobiotics like pesticides. Prochloraz is a widely used imidazole fungicide in horticulture and agriculture. A total of 60 juvenile common carp were divided into four groups of 15 fish and exposed to prochloraz at concentrations of 0, 50, 150 and 380 µg·l -1 , respectively. The total length of exposure was 28 days. Neopterin and biopterin concentrations were determined by reverse phase high performance liquid chromatography with fluorescence detection. Our results indicated that concentrations of neopterin (25 ± 7.6 nmol·l -1 ) and biopterin (190 ± 29 nmol·l -1 ) in plasma of untreated common carp were comparable with those in mammals. Neopterin concentrations significantly (P < 0.01) increased after exposure to prochloraz in comparison to non-exposed fish, while biopterin concentrations were not influenced by exposure to prochloraz. This study contains new data on neopterin and biopterin concentrations in fish plasma and investigates neopterin and biopterin in their non-traditional role as markers of cell mediated immunity of fish associated with pesticide exposure.

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“…The hsp70 responses are known to vary considerably according to tissue, organism, developmental stage, and stressor (Velazquez and Lindquist, 1984;Feder et al, 1996). For example, when juvenile chinook salmon, Oncorhynchus tshawytscha, were exposed to chlorpyrifos (organophosphate), a significant increase of hsp70 was observed in muscle tissue, although the level in gill tissue was significantly decreased (Eder et al, 2004). Induction of hsp70, which probably occurred in response to chlorfenapyr in a certain tissue, was masked in the whole organism in DBM.…”

Section: Discussionmentioning

confidence: 99%

Gene expression of hsp70 of the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae), in response to heat shock and insecticides

Sonoda

Tsumuki

2008

Appl. Entomol. Zool.

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The heat shock protein gene, hsp70, was cloned and sequenced from the diamondback moth, Plutella xylostella (L.). cDNA sequence analysis revealed a 1,992 bp-long open reading frame, which encodes a protein with a calculated molecular weight of 72.2 kDa. Results of Southern blot analysis suggested the presence of multiple copies of the hsp70 family in the genome. Increased expression of hsp70 was observed in fourth instar larvae exposed to 42°C. No significant up-regulation of hsp70 expression was observed when fourth instar larvae were treated with sub-lethal doses of insecticides, chlorfenapyr, permethrin, and acephate. These results suggest that hsp70 cloned in the present study is less sensitive to insecticides.

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Molecular and cellular biomarker responses to pesticide exposure in juvenile chinook salmon (Oncorhynchus tshawytscha)

Cited by 35 publications

References 8 publications

Multiscale approach of fish responses to different types of environmental contaminations: A case study

Multiscale approach of fish responses to different types of environmental contaminations: A case study

Effect of prochloraz fungicide on neopterin and biopterin concentrations in blood plasma of common carp

Gene expression of hsp70 of the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae), in response to heat shock and insecticides

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