2010
DOI: 10.1155/2010/498719
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Molecular and chemical characterization by Fourier transform infrared spectroscopy of human breast cancer cells with estrogen receptor expressed and not expressed

Abstract: Abstract. The present study was designed to identify and compare the infrared absorption spectra of two human breast cancer cell lines: MCF-7 (estrogen receptor expressed, ER+) and SKBr3 (estrogen receptor non-expressed, ER−). Comparison between SKBr3 and MCF-7 cells revealed differences in the following absorption band areas: 1087 cm −1 (DNA), 1397 cm

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Cited by 16 publications
(7 citation statements)
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“…To further analyze how protein conformation, rather than protein aggregation level, changes with aging, another independent and randomly chosen set of 127 samples from the pcb-Cohort was subjected to FTIR spectroscopy (TG2). Normalization of the FTIR spectra (Figure 3A) was carried out to ensure that differences in protein concentration between different samples would not affect spectral variance [37,38]. Amplification of the spectral region of amide I (1700-1600cm -1 ) (top) and corresponding 2 nd derivative spectra using the Savitzky-Golay algorithm with 3 smoothing points (bottom) is illustrated in Figure 3B.…”
Section: Ftir Spectra Overviewmentioning
confidence: 99%
“…To further analyze how protein conformation, rather than protein aggregation level, changes with aging, another independent and randomly chosen set of 127 samples from the pcb-Cohort was subjected to FTIR spectroscopy (TG2). Normalization of the FTIR spectra (Figure 3A) was carried out to ensure that differences in protein concentration between different samples would not affect spectral variance [37,38]. Amplification of the spectral region of amide I (1700-1600cm -1 ) (top) and corresponding 2 nd derivative spectra using the Savitzky-Golay algorithm with 3 smoothing points (bottom) is illustrated in Figure 3B.…”
Section: Ftir Spectra Overviewmentioning
confidence: 99%
“…In FTIR comparison with conditioned media of adipocytes (Figure 2A,B and Supplementary Figure S1A,B), amide I/amide II ratios were different between MBCM (2.42) and BPCM (3.96) (Figure 3A) [20,21]. It is believed that high amide I/amide II ratios affect protein pattern composition [22]. BPCM had the highest protein content (0.79) (Figure 3B) [20,21].…”
Section: Discussionmentioning
confidence: 97%
“…Their results suggest that cancer cells exhibit a stronger bonding structure in the membrane lipids compared to normal cells (70). In 2010, Mostaço-Guidolin and colleagues focused on the study of the MCF7 and SKBr3 cells, which are estrogen receptor positive and negative, respectively (71). Their results indicate that the MCF7 cell line shows increased absorbance intensity in the bands at 1085 (DNA), 1542 (amide II) and 1650 cm -1 (amide I), compared to the SKBr3 cell line.…”
Section: A Spectral Characteristics Of Cancer Cell Linesmentioning
confidence: 99%