Molecular and Metabolic Mechanism of Low-Intensity Pulsed Ultrasound Improving Muscle Atrophy in Hindlimb Unloading Rats

Sun, Lijun; An, Shasha; Zhang, Zhihao; Zhou, Yingshan; Yu, Yanan; Ma, Zhanke; Fan, Xiushan; Tang, Liang; Guo, Jianzhong

doi:10.3390/ijms222212112

Cited by 12 publications

(8 citation statements)

References 80 publications

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“…Based on the KEGG enrichment results, 50 metabolic pathways were enriched between the 10%FBS and 5%FBS+APE groups, and the top 20 metabolic pathways are listed in Table . In comparison to the 10%FBS group, the upregulated aspartate aminotransferase in the 5%FBS+APE group was involved in alanine, aspartate, and glutamate metabolism, as well as the phenylalanine, tyrosine, and tryptophan biosynthesis pathways (Table S4), and these two pathways are known to be important in the proliferation of myoblasts . On the other hand, casein kinase downregulated the Wnt signaling pathway in comparison to the 10%FBS group.…”

Section: Resultsmentioning

confidence: 97%

“…In comparison to the 10%FBS group, the upregulated aspartate aminotransferase in the 5%FBS+APE group was involved in alanine, aspartate, and glutamate metabolism, as well as the phenylalanine, tyrosine, and tryptophan biosynthesis pathways (Table S4), and these two pathways are known to be important in the proliferation of myoblasts. 43 On the other hand, casein kinase downregulated the Wnt signaling pathway in comparison to the 10%FBS group. Studies have demonstrated that the activation of the Wnt signaling pathway could promote cell proliferation and differentiation, 44 suggesting that APE supplementation might not fully replace serum and require additional bioactive substances.…”

Section: Proteomics Analysismentioning

confidence: 96%

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Integrated Omics Approach: Revealing the Mechanism of Auxenochlorella pyrenoidosa Protein Extract Replacing Fetal Bovine Serum for Fish Muscle Cell Culture

Dong,

Jiang,

Chang

et al. 2024

J. Agric. Food Chem.

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The process of producing cell-cultured meat involves utilizing a significant amount of culture medium, including fetal bovine serum (FBS), which represents a considerable portion of production expense while also raising environmental and safety concerns. This study demonstrated that supplementation with Auxenochlorella pyrenoidosa protein extract (APE) under low-serum conditions substantially increased Carassius auratus muscle (CAM) cell proliferation and heightened the expression of Myf5 compared to the absence of APE. An integrated intracellular metabolomics and proteomics analysis revealed a total of 13 and 67 differentially expressed metabolites and proteins, respectively, after supplementation with APE in the medium containing 5%FBS, modulating specific metabolism and signaling pathways, which explained the application of APE for passage cell culture under lowserum conditions. Further analysis revealed that the bioactive factors in the APE were protein components. Moreover, CAM cells cultured in reconstructed serum-free media containing APE, L-ascorbic acid, insulin, transferrin, selenium, and ethanolamine exhibited significantly accelerated growth in a scale-up culture. These findings suggest a promising alternative to FBS for fish muscle cell culture that can help reduce production costs and environmental impact in the production of cultured meat.

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Section: Resultsmentioning

confidence: 97%

Section: Proteomics Analysismentioning

confidence: 96%

Integrated Omics Approach: Revealing the Mechanism of Auxenochlorella pyrenoidosa Protein Extract Replacing Fetal Bovine Serum for Fish Muscle Cell Culture

Dong,

Jiang,

Chang

et al. 2024

J. Agric. Food Chem.

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“…The energy of LIFUS can be sent through the surface tissue and focused on the target tissue at a speci c depth, producing biological effects, and makeing tissue cell changes (11). Low-frequency and low-dose ultrasound do not produce destructive thermogenic reactions, it is more commonly used in the repairing muscle injury, promoting fracture healing, and in other elds of physiotherapy (12,13). LIFUS can promote uterine contraction and reduce the duration of postpartum lochia.…”

Section: Discussionmentioning

confidence: 99%

Effect of Ultrasound Therapy on Postpartum Uterine Involution: A Randomized Controlled Trial

Wei¹,

Wang²,

Yue³

et al. 2023

Preprint

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Background Short-term poor uterine involution manifests as uterine contraction weakness. This is one of the important causes of postpartum hemorrhage and is a serious threat to the life and safety of the mother. To investigate whether low-intensity focused ultrasound (LIFUS) could shorten lochia duration, relieve postpartum, and accelerate uterine involution compared with the sham treatment. Methods A multicenter, concealed, randomized, blinded, and sham-controlled clinical trial was conducted at three medical centers with 176 subjects, using a parallel group design. Subjects were enrolled from October 2019 to September 2020 and followed up for 42 days. The subjects who had normal prenatal checkups and met the inclusion and exclusion criteria were randomly allocated into a LIFUS or a sham group with a random sequence generated by a computer. Patients in the LIFUS group were given usual care with LIFUS treatmet, in which a LIFUS signal was transmitted to the uterus site through coupling gel, or sham treatment, in which low-intensity ultrasound signal output was not present. The duration of lochia, measured by weekly telephonic follow-ups after discharge, was the primary outcome. The involution of the uterus, determined by measuring the height of the fundus of the uterus, was the secondary outcome. Results Of the 256 subjects screened for eligibility, 176 were enrolled and randomly divided into the LIFUS group (n = 88) or the sham group (n = 88). Data on the height of the uterine fundus was derived from all the patients and 696/704 (99%) measurements were taken. Overall, a statistically significant difference was noted in time to lochia termination (hazard ratio: 2.65; 95% confidence interval [CI]: 1.82–3.85; P < 0.05). The height of the fundus showed significant difference following the second (mean difference: −1.74; 95% CI: −1.23 to − 2.25; P < 0.05) and the third treatment session (mean difference: −3.26; 95% CI: −2.74 to − 3.78; P < 0.05) after delivery. None of the subjects had any adverse reactions, such as skin damage and allergy during treatment. Conclusions This study revealed that LIFUS therapy could be considered as safe and effective. Additionally, it contributed to uterine involution and reduced the duration of postpartum lochia.

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“…Sun et al studied the effect of LIPUS on improving muscle atrophy by applying 30 mW/cm 2 and 80 mW/cm 2 of LIPUS. Their results showed that LIPUS had a tendency to prevent muscle strength loss, but there was no significant difference [ 28 ]. We used sound intensities of 50 mW/cm 2 and 150 mW/cm 2 here to stimulate muscles.…”

Section: Discussionmentioning

confidence: 99%

Integrated Application of Low-Intensity Pulsed Ultrasound in Diagnosis and Treatment of Atrophied Skeletal Muscle Induced in Tail-Suspended Rats

Yang

Lei

et al. 2022

IJMS

Self Cite

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Long-term exposure to microgravity leads to muscle atrophy, which is primarily characterized by a loss of muscle mass and strength and reduces one′s functional capability. A weightlessness-induced muscle atrophy model was established using the tail suspension test to evaluate the intervention or therapeutic effect of low-intensity pulsed ultrasound (LIPUS) on muscle atrophy. The rats were divided into five groups at random: the model group (B), the normal control group (NC), the sham-ultrasound control group (SUC), the LIPUS of 50 mW/cm2 radiation group (50 UR), and the LIPUS of 150 mW/cm2 radiation group (150 UR). Body weight, gastrocnemius weight, muscle force, and B-ultrasound images were used to evaluate muscle atrophy status. Results showed that the body weight, gastrocnemius weight, and image entropy of the tail suspension group were significantly lower than those of the control group (p < 0.01), confirming the presence of muscle atrophy. Although the results show that the muscle force and two weights of the rats stimulated by LIPUS are still much smaller than those of the NC group, they are significantly different from those of the pure tail suspension B group (p < 0.01). On day 14, the gastrocnemius forces of the rats exposed to 50 mW/cm2 and 150 mW/cm2 LIPUS were 150% and 165% of those in the B group. The gastrocnemius weights were both 135% of those in the B group. This suggests that ultrasound can, to a certain extent, prevent muscular atrophy.

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Molecular and Metabolic Mechanism of Low-Intensity Pulsed Ultrasound Improving Muscle Atrophy in Hindlimb Unloading Rats

Cited by 12 publications

References 80 publications

Integrated Omics Approach: Revealing the Mechanism of Auxenochlorella pyrenoidosa Protein Extract Replacing Fetal Bovine Serum for Fish Muscle Cell Culture

Integrated Omics Approach: Revealing the Mechanism of Auxenochlorella pyrenoidosa Protein Extract Replacing Fetal Bovine Serum for Fish Muscle Cell Culture

Effect of Ultrasound Therapy on Postpartum Uterine Involution: A Randomized Controlled Trial

Integrated Application of Low-Intensity Pulsed Ultrasound in Diagnosis and Treatment of Atrophied Skeletal Muscle Induced in Tail-Suspended Rats

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