Molecular and serological detection of Theileria equi and Babesia caballi infection in horses and ixodid ticks in Iran

Abedi, Vali; Razmi, Gholam Reza; Seifi, Hesam A; Naghibi, Abolghasem

doi:10.1016/j.ttbdis.2013.11.008

Cited by 42 publications

(39 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, we were not able to identify any study conducted in Portugal that has isolated T. annulata from R. bursa ticks. T. equi (formerly known as Babesia equi) can be naturally transmitted by ticks of the family Ixodidae (Stiller et al, 2002) and R. bursa has been implicated as the vector of piroplasmosis caused by T. equi in horses in Spain and Iran (Abedi et al, 2014;Garcia-Sanmartin et al, 2008). Similarly, we have found T. equi in ticks of this species infesting horses.…”

Section: Discussionsupporting

confidence: 71%

Detection and phylogenetic characterization of Theileria spp. and Anaplasma marginale in Rhipicephalus bursa in Portugal

Ferrolho

Antunes

Santos

et al. 2016

Ticks and Tick-borne Diseases

View full text Add to dashboard Cite

a b s t r a c tTicks are obligatory blood-sucking arthropod (Acari:Ixodida) ectoparasites of domestic and wild animals as well as humans. The incidence of tick-borne diseases is rising worldwide, challenging our approach toward diagnosis, treatment and control options. Rhipicephalus bursa Canestrini and Fanzago, 1877, a two-host tick widely distributed in the Palearctic Mediterranean region, is considered a multi-host tick that can be commonly found on sheep, goats and cattle, and occasionally on horses, dogs, deer and humans. R. bursa is a species involved in the transmission of several tick-borne pathogens with a known impact on animal health and production. The aim of this study was to estimate R. bursa prevalence in Portugal Mainland and circulating pathogens in order to contribute to a better knowledge of the impact of this tick species. Anaplasma marginale and Theileria spp. were detected and classified using phylogenetic analysis. This is the first report of Theileria annulata and Theileria equi detection in R. bursa ticks feeding on cattle and horses, respectively, in Portugal. This study contributes toward the identification of currently circulating pathogens in this tick species as a prerequisite for developing future effective anti-tick control measures.

show abstract

Section: Discussionsupporting

confidence: 71%

Detection and phylogenetic characterization of Theileria spp. and Anaplasma marginale in Rhipicephalus bursa in Portugal

Ferrolho

Antunes

Santos

et al. 2016

Ticks and Tick-borne Diseases

View full text Add to dashboard Cite

show abstract

“…In our previous study the molecular prevalence of T. equi was 22.86% in horses in central Iran (Bahrami et al 2014). Abedi et al (2014) found 45% of horses on the north-eastern border of Iran to be infected with T. equi. Thus, overall, it has been demonstrated that T. equi is present in horses in Iran.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Theileria equi in Persian Arab horses from Iran

Bahrami¹,

Ghadrdan²,

Borujeni³

et al. 2014

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The Khuzestan province in south-western Iran is the centre of Persian Arab horse breeding and training. The present study was aimed at determining the prevalence of Theileria equi in the equids of this province. A total of 165 blood samples from healthy Persian Arab horses from twenty four stables were examined for the presence of T. equi infection using molecular methods. For detection of T. equi, primers targeting the 18SrRNA gene were selected. The PCR method gave 47 (28.5%) positive results. Age (P = 0.68), sex (P = 0.88), contact with cattle (P = 0.26) and type of activity (P = 0.06) were not determined as risk factors for T. equi infection in this study. However, there was a significant geographical variation in the prevalence of T. equi infection ranging from 8.3% (2/24) in Shushtar to 55.6% (10/18) in Ramhormoz (CI, 2.46-76.82) (P = 0.003). In conclusion, equine theileriosis has the potential of posing a significant problem for Iran's Persian Arab horse industry and should remain a major concern to the horse community and regulatory agencies.

show abstract

“…Devido a baixa sensibilidade deste método, testes imunológicos indiretos e métodos diretos como a Reação em Cadeia da Polimerase -PCR (Polymerase Chain Reaction) tem sido incorporados com sucesso na rotina de laboratórios veterinários (Maroso et al 2002, Golynski et al 2008, Salvagni et al 2010, Leal et al, 2011, Abedi et al, 2014, Laus et al 2015, Malekifard et al 2014, Posada-Guazmán et al 2015, Sgorbini et al 2015. Provas sorológicas, como a Reação de Imunofluorescência Indireta (RIFI) e o Ensaio Imunoenzimático (ELISA) acusam que o animal teve contato com o agente em alguma fase da vida, mas não necessariamente que são animais doentes, podendo ou não estar com o agente no seu organismo no momento da colheita, induzindo a ativação do sistema imunológico (Parra 2009, Abedi et al 2014, Laus et al 2015, Sgorbini et al 2015.…”

Section: Introductionunclassified

Investigação clínica e comparação do esfregaço sanguíneo e PCR para diagnóstico de hemoparasitas em equinos de esporte e tração (carroceiros)

et al. 2016

View full text Add to dashboard Cite

Resumo: Na clínica médica de equinos, explora-se o perfil hematológico do animal, geralmente, com a finalidade de encontrar alterações que não foram constatadas ao exame clínico. A pesquisa de hematozoários em equinos, muitas vezes, apresenta resultados conflitantes entre o quadro clínico apresentado pelo animal e o resultado laboratorial, levantando a hipótese de que a técnica de pesquisa de hematozoários seja a responsável por falhas diagnósticas. Este estudo visa comparar os valores obtidos em exames hematológicos de 15 equinos de esporte e 15 equinos de tração (carroceiros), levando-se em consideração diferenças como características nutricionais, estado de higidez e tipo de atividade realizada, e comparar as diferentes técnicas de pesquisa de hematozoários, como esfregaço sanguíneo e PCR. Verificou-se que apenas os equinos de tração apresentaram valores médios de hemácias, hematócrito e hemoglobina abaixo do considerado fisiológico para a espécie, embora 100% dos animais, de ambos os grupos experimentais, tenham sido considerados positivos para hemoparasitoses por PCR. Verifica-se a superioridade do método de pesquisa de hemoparasitas por PCR, em comparação com esfregaço sanguíneo, realizado por diferentes técnicas, visto que apenas 33,3% dos animais foram considerados positivos para Theileria equi por esta técnica, enquanto que o PCR revelou 100% de positividade, para Theileria equi, Babesia caballi e infecção mista. Nenhum dos animais estudados foi diagnosticado com Anaplasma phagocytophilum (Ehrlichia equi) e Ehrlichia risticcii (Neoricketsia risticii). Verifica-se, então, que muitos dos diagnósticos de ausência de hemoparasitose por exame hematológico e ou esfregaço sanguíneo são errôneos, devido à baixa sensibilidade da técnica e podem repercutir em falha no tratamento ou disseminação dos hemoparasitos e das hemoparasitoses. Ressalta-se, então, a importância de exames como o PCR na elaboração de diagnóstico definitivo.

show abstract

Molecular and serological detection of Theileria equi and Babesia caballi infection in horses and ixodid ticks in Iran

Cited by 42 publications

References 40 publications

Detection and phylogenetic characterization of Theileria spp. and Anaplasma marginale in Rhipicephalus bursa in Portugal

Detection and phylogenetic characterization of Theileria spp. and Anaplasma marginale in Rhipicephalus bursa in Portugal

Epidemiology of Theileria equi in Persian Arab horses from Iran

Investigação clínica e comparação do esfregaço sanguíneo e PCR para diagnóstico de hemoparasitas em equinos de esporte e tração (carroceiros)

Contact Info

Product

Resources

About