Molecular and Ultrastructural Characterization of Porcine Hippurate-Negative
            <i>Brachyspira pilosicoli</i>

Fossi, Marja; Pohjanvirta, Tarja; Sukura, Antti; Heinikainen, Sirpa; Lindecrona, R. H.; Pelkonen, Sinikka

doi:10.1128/jcm.42.7.3153-3158.2004

Cited by 14 publications

(10 citation statements)

References 36 publications

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“…However, occasional indole-negative B. hyodysenteriae and hippurate-negative B. pilosicoli isolates have been reported. 6,7 Using the commercial testing system, j 2 different enzymatic profiles were observed. The split of isolates based on the 2 different enzymatic profiles nearly matched the separation of NSH isolates based on nox and 16S rRNA sequence analyses, though there were a few exceptions; while most isolates from NSH clade I were positive for β-glucosidase (16/17), most isolates from NSH clade II were negative for this enzyme (2/3).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular characterization of a novel strongly hemolytic Brachyspira species, provisionally designated “Brachyspira hampsonii”

et al. 2012

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Abstract. Since 2007, outbreaks of severe bloody diarrhea and hemorrhagic colitis have been reported in the United States and Canada. Though the primary causative agent of swine dysentery is Brachyspira hyodysenteriae, which is strongly hemolytic, the current report describes the isolation of a novel strongly hemolytic Brachyspira sp. This novel Brachyspira sp. was identified from clinical submissions at the Minnesota Veterinary Diagnostic Laboratory, and 40 of such isolates were obtained from 22 clinical submissions representing 5 states. Isolates were confirmed to be different from any known Brachyspira sp. on the basis of phylogenetic analysis of nucleotide sequences of nox and 16S ribosomal RNA (rRNA) genes. Phylogenetic analyses grouped all isolates into 2 clades (clades I and II), and grouping patterns were similar for both nox and 16S rRNA gene sequence analyses. Phenotypically, all isolates were indole and hippurate negative, and enzymatic profiling indicated 2 types of profiles, irrespective of the phylogenetic grouping, differing only in the production of β-glucosidase. The results suggest that a potentially virulent new species of Brachyspira sp., provisionally named "Brachyspira hampsonii ", is circulating among swine herds in the United States.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular characterization of a novel strongly hemolytic Brachyspira species, provisionally designated “Brachyspira hampsonii”

et al. 2012

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“…; Fossi et al . ). Other authors have described difficulties with regard to the biochemical identification of certain Brachyspira species (Rohde et al .…”

Section: Resultsmentioning

confidence: 97%

MALDI-TOF MS for identification of porcine Brachyspira species

Prohaska

Pflüger²,

Ziegler³

et al. 2013

Lett Appl Microbiol

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Significance and Impact of the Study: Current methods for the discrimination of pathogenic Brachyspira hyodysenteriae and Brachyspira pilosicoli from Brachyspira species with low pathogenic potential have proven to be laborious and time-consuming and are therefore not suitable for routine diagnostics. This study describes the evaluation of MALDI-TOF MS for the identification of different porcine Brachyspira species in routine diagnostic laboratories. The results suggest that MALDI-TOF MS is an effective method for the identification of porcine Brachyspira spp. and accelerates diagnosis of swine dysentery and porcine intestinal spirochaetosis. AbstractThe aim of this study consisted in evaluating MALDI-TOF MS as a tool for the identification of the genus Brachyspira (B.) and its relevant species for the pig industry. First, a database was created with 30 control strains, and superspectra for five different porcine Brachyspira species were calculated. In a second step, 67 field isolates were investigated using MALDI-TOF MS, and results were compared to those obtained using nox gene-based RFLP (reference method) and biochemical tests. Among the 67 field isolates, five different Brachyspira species were detected using nox gene-based RFLP analysis. MALDI-TOF MS analysis correctly assigned all isolates to the genus Brachyspira and identified all isolates from B. hyodysenteriae (29/29), B. pilosicoli (11/11), B. intermedia (4/4) and B. innocens (11/11). In terms of B. murdochii, MALDI-TOF MS assigned one of 12 isolates ambiguously as B. innocens/B. murdochii. The results of this study indicate that MALDI-TOF MS facilitates the diagnosis of swine dysentery and porcine intestinal spirochaetosis.

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“…B. pilosicoli usually hydrolyses hippurate, which is a major criterion for the biochemical differentiation of B. pilosicoli from the other porcine Brachyspira species [ 8 ]. However, some porcine B. pilosicoli strains in Finland are hippurate-negative and their pathogenicity is unclear since other potentially enteropathogenic bacteria, such as Lawsonia intracellularis and B. pilosicoli with a positive hippurate reaction, have simultaneously been diagnosed in the herds [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Neither Hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli Type Strain Caused Diarrhoea in Early-weaned Pigs by Experimental Infection

Fossi

Ahlsten

Pohjanvirta³

et al. 2005

Acta Vet Scand

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Sukura A, Pelkola K, Pelkonen S: Neither hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli type strain caused diarrhoea in early-weaned pigs by experimental infection. Acta vet. scand. 2005, 46, 257-267. -A hippurate-negative biovariant of Brachyspira pilosicoli (B. pilosicoli hipp-) is occasionally isolated in diarrhoeic pigs in Finland, often concomitantly with hippurate-positive B. pilosicoli or Lawsonia intracellularis. We studied pathogenicity of B. pilosicoli hipp-with special attention paid to avoiding co-infection with other enteric pathogens. Pigs were weaned and moved to barrier facilities at the age of 11 days. At 46 days, 8 pigs were inoculated with B. pilosicoli hipp-strain Br1622, 8 pigs were inoculated with B. pilosicoli type strain P43/6/78 and 7 pigs were sham-inoculated. No signs of spirochaetal diarrhoea were detected; only one pig, inoculated with P43/6/78, had soft faeces from day 9 to 10 post inoculation. The pigs were necropsied between days 7 and 23 after inoculation. Live pigs were culturenegative for Brachyspira spp., but B. pilosicoli hipp-was reisolated from necropsy samples of two pigs. The lesions on large colons were minor and did not significantly differ between the three trial groups. In silver-stained sections, invasive spirochaetes were detected in colonic mucosae of several pigs in all groups. Fluorescent in situ hybridisation for genus Brachyspira, B. pilosicoli and strain Br1622 was negative. However, in situ detection for members of the genus Leptospira was positive for spirochaete-like bacteria in the colonic epithelium of several pigs in both infected groups as well as in the control group. L. intracellularis, Salmonella spp., Yersinia spp. and intestinal parasites were not detected. The failure of B. pilosicoli strains to cause diarrhoea is discussed with respect to infectivity of the challenge strains, absence of certain intestinal pathogens and feed and management factors.Brachyspira pilosicoli, intestinal spirochaetosis, spirochaetal colitis, pig, diarrhoea, experimental infection, early weaning, Lawsonia intracellularis.

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Molecular and Ultrastructural Characterization of Porcine Hippurate-Negative Brachyspira pilosicoli

Cited by 14 publications

References 36 publications

Phenotypic and molecular characterization of a novel strongly hemolytic Brachyspira species, provisionally designated “Brachyspira hampsonii”

Phenotypic and molecular characterization of a novel strongly hemolytic Brachyspira species, provisionally designated “Brachyspira hampsonii”

MALDI-TOF MS for identification of porcine Brachyspira species

Neither Hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli Type Strain Caused Diarrhoea in Early-weaned Pigs by Experimental Infection

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