Molecular Breeding for the Development of Blast and Bacterial Blight Resistance in Rice cv. IR50

Narayanan, Narayanan N.; Baisakh, Niranjan; Cruz, C. M. Vera; Gnanamanickam, S. S.; Datta, Karabi; Datta, Swapan K.

doi:10.2135/cropsci2002.2072

Cited by 94 publications

(41 citation statements)

References 27 publications

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“…It should be noted, however, that such explants are not necessarily equally efficient in their response. Transformation efficiency also Narayanan et al (2002Narayanan et al ( , 2004 and Tu et al (1998bTu et al ( , 2000a depends upon the regeneration capacity and the efficiency of selection, which in turn depends upon how different explants are handled. In most crops, selection is based on the use of antibiotics or herbicides, but chemical selection is not always necessary.…”

Section: Particle Bombardment Has No Biological Constraints or Host Lmentioning

confidence: 99%

Particle bombardment and the genetic enhancement of crops: myths and realities

et al. 2005

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DNA transfer by particle bombardment makes use of physical processes to achieve the transformation of crop plants. There is no dependence on bacteria, so the limitations inherent in organisms such as Agrobacterium tumefaciens do not apply. The absence of biological constraints, at least until DNA has entered the plant cell, means that particle bombardment is a versatile and effective transformation method, not limited by cell type, species or genotype. There are no intrinsic vector requirements so transgenes of any size and arrangement can be introduced, and multiple gene cotransformation is straightforward. The perceived disadvantages of particle bombardment compared to Agrobacterium-mediated transformation, i.e. the tendency to generate large transgene arrays containing rearranged and broken transgene copies, are not borne out by the recent detailed structural analysis of transgene loci produced by each of the methods. There is also little evidence for major differences in the levels of transgene instability and silencing when these transformation methods are compared in agriculturally important cereals and legumes, and other non-model systems. Indeed, a major advantage of particle bombardment is that the delivered DNA can be manipulated to influence the quality and structure of the resultant transgene loci. This has been demonstrated in recently reported strategies that favor the recovery of transgenic plants containing intact, singlecopy integration events, and demonstrating high-level transgene expression. At the current time, particle bombardment is the most efficient way to achieve plastid transformation in plants and is the only method so far used to achieve mitochondrial transformation. In this review, we discuss recent data highlighting the positive impact of particle bombardment on the genetic transformation of plants, focusing on the fate of exogenous DNA, its organization and its expression in the plant cell. We also discuss some of the most important applications of this technology including the deployment of transgenic plants under field conditions.

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Section: Particle Bombardment Has No Biological Constraints or Host Lmentioning

confidence: 99%

Particle bombardment and the genetic enhancement of crops: myths and realities

et al. 2005

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“…have fastened the procedure for gene introgression and gene pyramiding. The R-genes like Pi1, Pi5, Piz-5 and Pita [43, 70,75,84] have been introgressed in various elite rice genotypes using MAS.…”

Section: Introgression Of Blast Resistance Genes In Commercial Cultivarsmentioning

confidence: 99%

Rice Blast Management Through Host-Plant Resistance: Retrospect and Prospects

et al. 2012

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Rice (Oryza sativa) plays a significant role in achieving global food security. However, it suffers from several biotic and abiotic stresses that seriously affect its production. Rice blast caused by hemibiotropic fungal pathogen Magnaporthe oryzae is one of the most widespread and devastating diseases of rice. The crop rice is vulnerable to this pathogen from seedlings to adult plant stages affecting leaves, nodes, collar, panicles and roots. This disease can be effectively managed through host resistance. Of the 100 blast resistance genes, identified and mapped in different genotypes of rice, 19 genes have been cloned and characterized at the molecular level. Most of these genes belong to nucleotide binding sites and leucine rich repeats. Besides more than 350 quantitative trait loci (QTLs) have also been identified in the rice genome. These blast resistance genes and QTLs have been successfully mobilized in the commercial cultivars by using standard plant breeding techniques and also by marker assisted backcross breeding. With the advent of latest molecular biology techniques and our understanding of the basic mechanisms of Magnaporthe-rice pathosystem, the strategies for broadspectrum resistance to M. oryzae can be designed in future.

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“…It is also applied to identify markers tightly linked to blast resistance genes and to detect genes and QTLs on rice chromosomes (Fjellstrom et al 2004a, b;Zhu et al 2004;Liu et al 2005). Molecular breeding approach involving DNA markers, QTLs mapping, markeraided selection (MAS) and genetic transformation for resistance against blast or other traits has been used in developing a new improved cultivated rice by many rice researchers and breeders (Narayanan et al 2002;Mallikarjuna and Sarla 2010;Zhao et al 2010). PCR-based DNA markers now are used as a low-cost, highthroughput alternative to conventional phenotypic screening for direct detection of disease resistance genes, allowing rapid introgression of genes into susceptible varieties as well as the incorporation of multiple genes into individual lines for more durable blast resistance.…”

Section: Introductionmentioning

confidence: 99%

SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryza sativa L.)

et al. 2011

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Rice blast caused by the fungus Magnaporthe oryzae is one of the most devastating diseases of rice in nearly all rice growing areas of the world including Malaysia. To develop cultivars with resistance against different races of M. oryzae, availability of molecular markers along with marker-assisted selection strategies are essential. In this study, 11 polymorphic simple sequence repeat (SSR) markers with good fit of 1:2:1 ratio for single gene model in F 2 population derived from the cross of Pongsu seribu 2 (Resistant) and Mahsuri (Susceptible) rice cultivars were analysed in 296 F 3 families derived from individual F 2 plants to investigate association with Pi gene conferring resistance to M. oryzae pathotype. Parents and progeny were grouped into two phenotypic classes based on their blast reactions. Chi-square test for the segregation of resistance and susceptibility in F 3 generation fitted a ratio of approximately 3:1. Association of SSR markers with phenotypic trait in F 3 families was identified by statistical analysis. Four SSR markers (RM413, RM5961, RM1233 and RM8225) were significantly associated with blast resistance to pathotype 7.2 of M. oryzae in rice (p≤0.01). These four markers accounted for about 20% of total phenotypic variation. So, these markers were confirmed as suitable markers for use in marker-assisted selection and confirmation of blast resistance genes to develop rice cultivars with durable blast resistance in Malaysian rice breeding programmes.

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Molecular Breeding for the Development of Blast and Bacterial Blight Resistance in Rice cv. IR50

Cited by 94 publications

References 27 publications

Particle bombardment and the genetic enhancement of crops: myths and realities

Particle bombardment and the genetic enhancement of crops: myths and realities

Rice Blast Management Through Host-Plant Resistance: Retrospect and Prospects

SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryza sativa L.)

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