Molecular changes in particulate organic matter (POM) in a typical Chinese paddy soil under different long‐term fertilizer treatments

Abstract

A combination of solid-state CPMAS-13 C-NMR and TMAH thermochemolysis-GC/MS was applied to investigate the molecular composition of particulate organic matter (POM) separated from a Chinese paddy soil, from the Tai Lake region, under a long-term field experiment with different fertilizer treatments. The treatments were: (i) no fertilizer application (NF), (ii) chemical fertilizers only (CF), (iii) chemical fertilizer plus pig manure (CFM) and (iv) chemical fertilizer plus crop straw (CFS). CPMAS-13 C-NMR spectra showed that POM from all treated plots was rich in O-alkyl-C compounds, followed by alkyl-C and aromatic-C compounds. However, as compared with a control (NF), POM under CFM and CFS treatments exhibited a smaller relative O-alkyl-C content and a larger contribution of aromatic-C and alkyl-C, thus increasing both aromaticity and hydrophobicity and, hence, recalcitrance of POM samples. Thermochemolysis of POM from all treatments demonstrated a dominance of aliphatic and lignin-derived compounds. However, the distribution of lignin monomers (p-hydroxyphenyl, P, guaiacyl, G, and syringyl, S) revealed significant differences among the treatments. The relative distribution of lignin P, G and S monomers in NF, CF and CFS indicated a preferential contribution of annual crops and maize straw, as compared with that found for CFM. Concomitantly, a larger content of aliphatic thermochemolysis derivatives was found for CFS and CFM. The relative increase of aliphatic molecules in CFS was attributed to hydrophobic polyesters from higher plants. In the CF and CFM systems, the presence of aliphatic components of microbial origin suggested a greater microbial activity in comparison with NF and CFS. The combined application of solid state CPMAS-13 C-NMR and TMAH thermochemolysis-GC/MS can be used to assess effectively the accumulation of recalcitrant organic compounds in soil POM under long-term fertilizer application with organic biomass. It is thus inferred that soil organic matter stabilization by molecular recalcitrance contributes to carbon sequestration in Chinese paddy soils under long-term managements.

The role of non‐crystalline Fe in the increase of SOC after long‐term organic manure application to the red soil of southern China

“…The hydrophobicity of SOC causes water repellency in soil aggregates and decreases the wettability, which might reduce SOC decomposition rates by reducing SOC accessibility (Lützow et al, 2006). Zhou et al (2010) also suggested that aliphatic hydrophobic components providing molecular recalcitrance contributed to SOC stabilization in Chinese paddy soils. However, environmental conditions such as soil pH, moisture and anaerobicity could 0-20 cm CK 9.3 10.1 0.9 N 9 .…”

Influence of extractants and filter materials in the extraction of dissolved organic matter (DOM) from subtropical agricultural soil

“…Mass spectra (1 scan/s) were recorded under electron impact at 70 eV. Compound identification was conducted based on comparisons of mass spectra with the NIST-library (National Institute of Standards and Technology Mass Spectral Library) database, published spectra, and authentic standards (Saiz-Jimenez, 1994;Sihombing et al, 1996;Zhou et al, 2010)., We correlated the pyrolysis products with compound types in the original DOM solutions according to the pyrogram. Products not detected in samples were given a zero value in the statistical treatments.…”

Carbon and nitrogen forms in soil organic matter influenced by incorporated wheat and corn residues

“…Aromaticity, calculated as the sum of aromatic C-O and aromatic C percentages measured by NMR (Dai et al 2001;Zhou et al 2010), is shown in Table 3. Compared with the CK and F treatments, the aromaticity of the SOM decreased from 20 to 18.3 in the treatment of crop residue incorporation plus fertilizer (i.e., SF3).…”