Molecular Characteristics of Water-Soluble Extracts fromHypsizigus marmoreusand Theirin VitroGrowth Inhibition of Various Cancer Cell Lines and Immunomodulatory Function in Raw 264.7 Cells

Characterization and immunomodulatory activities of polysaccharides from Spirogyra neglecta (Hassall) Kützing

“…before. [28][29][30] In general, the proximate compositions of the macroalgae were significantly different depending on species, growing conditions, extraction procedures, and analytical methods. Among these factors, the variation in their proximate compositions seemed to be largely influenced by the species differences.…”

Characterization and immunomodulatory activities of sulfated polysaccharides from Capsosiphon fulvescens

“…Partially methylated alditol acetates were prepared from fully methylated samples by acid hydrolysis with 4 M TFA at 100 • C for 6 h, followed by a reduction of the hydrolysates in water using NaBD 4 and an acetylation with acetic anhydride. The partially methylated alditol acetates were analyzed by a gas chromatography-mass spectrometry (GC-MS) (6890 N/MSD 5973, Agilent Technologies, Santa Clara, CA) using a HP-5MS capillary column (30 m × 0.25 mm × 0.25 m) (Agilent Technologies, Santa Clara, CA) under previously reported conditions [22].…”

“…The cells were plated in a 96-well microplate, and cultured for 4 h at 37 • C in the presence of 5% CO 2 . The sample solution (100 L) at concentrations of 50, 100 or 200 g/mL was added to the wells in triplicate, and the cells were cultured for 72 h. After this incubation, the anticancer activity of the polysaccharides was determined using the WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2 H-5-tetrazolio]-1,3-benzene disulfonate) colorimetric assay kit (Roche Diagnostics, Madison, WI, USA) under previously reported conditions [22].…”

Influence of Treatment with Extracts of <i>Hypsyzigus marmoreus</i> Mushroom on Body Composition during Obesity Development in KK-A^y Mice

“…To test these speculations, we will in future conduct further studies under paired-feeding conditions with examination of energy expenditure. However, an anti-proliferative effect of EHM on adipocytes, possible components of which include hypsiziprenol, hypsin, or polysaccharides as demonstrated by previous studies in vivo only (7)(8)(9)(10)17) cannot be ruled out. Moreover, dietary polyphenols, such as resveratrol, were reported to increase the expression of the UCP or other obesity-related genes, including those responsible for ␤-oxidation and lipolysis, whose expressions were unchanged in both groups (data not shown) (18), and thereby to prevent obesity.…”

“…HM has several basic functions, such as antitumor (7)(8)(9)(10), antioxidant (11), antiinfl ammatory (12), anti-allergy (11,12) and immunostimulant (13) effects. On the other hand, the potentially useful effect of HM in diabetic mice has not as yet been studied.…”