Molecular characterization and antimicrobial resistance of Salmonella enterica from swine slaughtered in two different types of Philippine abattoir

Calayag, Alyzza Marie B.; Paclibare, Phyllis Anne P.; Santos, Pauline Dianne; Bautista, Corinne Aimee C.; Rivera, Windell L.

doi:10.1016/j.fm.2017.01.016

Cited by 28 publications

(41 citation statements)

References 25 publications

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“…[ 22 ]. Carbapenems are the final choice of antibiotics used in the treatment of salmonellosis when the bacteria exhibit resistance to antibiotics such as ciprofloxacin and CRO [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

2020

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Background and Aim: Salmonella spp. are one of the most important food-borne pathogens in the world, emerging as a major public health concern. Moreover, multidrug-resistant (MDR) strains have been isolated from salmonellosis outbreaks, which compromise its treatment success. This study was conducted to characterize the phenotypic and genotypic antibiotic resistance profile of Salmonella strains isolated from broilers and humans from the regions of Tolima and Santander (Colombia). Materials and Methods: Salmonella spp. strains (n=49) were confirmed through molecular detection by amplification of the invA gene. Phenotypic antibiotic resistance was determined by the automated method and the agar diffusion method, and the presence of resistance genes was evaluated by PCR. Genotypic characterization was conducted using the enterobacterial repetitive intergenic consensus (ERIC)-PCR method, from which a dendrogram was generated and the possible phylogenetic relationships were established. Results: Salmonella isolates were classified as MDR strains exhibiting resistance to four antibiotic classes, penicillins, aminoglycosides, sulfonamides, and cephalosporins, and the human strains were resistant to gentamicin. At the genotypic level, the isolates contained the genes blaCMY2, blaCTX-M, blaPSE-1, blaTEM, aadA1, srtB, dfrA1, sul2, and floR. The genotyping results obtained by ERIC-PCR allowed the grouping of strains according to the source of isolation. Conclusion: The Salmonella spp. strains exhibited resistance to multiple antibiotics, as well as multiple genes associated with them, and the ERIC-PCR method was a technique that was helpful in generating clusters with biological significance.

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“…[ 22 ]. Carbapenems are the final choice of antibiotics used in the treatment of salmonellosis when the bacteria exhibit resistance to antibiotics such as ciprofloxacin and CRO [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

2020

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show abstract

“…Initial Salmonella contamination of animals from farms, slaughterhouses, and distribution may also have affected the different rates of Salmonella-contamination in wet markets. In previous Salmonella prevalence studies in abattoirs, varying Salmonella frequencies in slaughtered swine were observed in different slaughterhouses in Metro Manila [14,15]. We strongly suggest implementing strict hazard analysis and critical control point (HACCP) practices in slaughterhouses and transport.…”

Section: Plos Onementioning

confidence: 73%

“…And frequently isolated in apparently healthy cattle in Australasia (5.4%) and Africa (2.1%) [57]. Other Salmonella serovars such as S. Newport, S. Stanley, S. Derby, S. Weltevreden, and S. Enteritidis were previously reported in animal and retail meat [15,31,53,54], while S. Senftenberg and S. Hvittingfoss were reported in peanut butter products in the Philippines. These detected serovars can pose risks to retail meat vendors and consumers through unhygienic handling, cross-contamination with ready-to-eat food such as fresh produce, and consumption of poorly cooked meat.…”

Section: Plos Onementioning

confidence: 98%

“…studies in Metro Manila demonstrated that Salmonella serogroup O:3 was the most dominant serogroup detected in raw and processed meat (78.57%) from wet markets [13], and slaughtered swine (75%) from slaughterhouses [15]. However, Ng & Rivera [14] found that serogroup O:4 (73%) was the most frequently isolated serogroup in slaughtered swine in abattoirs followed by serogroup O:3 (24%).…”

Section: Plos Onementioning

confidence: 99%

“…Significantly higher number of Salmonella-contaminated samples were observed in raw meat (p = 0.00001) (85.77%, n = 235) compared to processed meat samples (40.36%, n = 180). High prevalence of Salmonella contamination in ground pork can be associated with high frequency (45%, n = 240) of Salmonella found in slaughtered swine from different Metro Manila abattoirs [14,15]. Data regarding Salmonella frequencies in chicken and cows from slaughterhouses and farms are currently lacking.…”

Section: Presence and Distribution Of Salmonella In Retail Meatmentioning

confidence: 99%

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