Molecular characterization and drug susceptibility of non-O1/O139 V. cholerae strains of seafood, environmental and clinical origin, Italy

Ottaviani, Donatella; Medici, Laura; Talevi, Giulia; Napoleoni, Maira; Serratore, Patrizia; Zavatta, Emanuele; Bignami, Giorgia; Masini, Laura; Chierichetti, Serena; Fisichella, Stefano; Leoni, Francesca

doi:10.1016/j.fm.2017.11.011

Cited by 23 publications

(25 citation statements)

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“…These variance reports might be associated with the region or environmental activities in their individual isolation sites or the flexible and changing nature of V. cholerae in diverse environment [ 64 ]. In the reports of Guevara et al [ 65 ], resistance was not observed amongst the Vibrio strains but the trend changed over time as subsequent simultaneous report between 1992 and 2017 shows that [ 59 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 ] V. cholerae is emerging in resistant profile and multiple antibiotic resistance which have also extended from penicillanases or β-lactamases of various class to carbapenemases as affirmed during their study. This is further affirmed in this study as resistance was reported for extended spectrum β-lactamase and other novel carbapenemase resistance.…”

Section: Discussionmentioning

confidence: 76%

Antibiotic Susceptibility Testing (AST) Reports: A Basis for Environmental/Epidemiological Surveillance and Infection Control Amongst Environmental Vibrio cholerae

Igere

Okoh

Nwodo

2020

IJERPH

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Distribution, investigation, surveillance and control (DISC) of cholera outbreaks in endemic/non-endemic regions has been a concerted approach towards the management of the causal pathogen. Relevant organization, government, health systems and the public have implemented several steps towards controlling the menace, yet pathogen continues to occur with diverse phenotypes/genotypes of high clinical and epidemiological relevance. The study determines antibiotic susceptibility/resistance pattern of Vibrio cholerae isolates retrieved from six domestic water sources between March and August 2018. Serological and molecular typing methods (polymerase chain reaction or PCR) were used to confirm the isolates identity. Antibiotic susceptibility testing was conducted using six commonly employed antibiotics of V. cholerae according to the recommendation of Clinical Laboratory Standard and European Committee for Antimicrobial Susceptibility Testing with other relevant antibiotics of investigative epidemiology and infection control, employing both disc diffusion test and PCR gene detection. Samples presumptive counts ranged between 1.10 to 7.91 log10 CFU/mL. Amongst the 759 presumptive isolates retrieved, sixty-one were confirmed as V. cholerae which were further serogrouped as Non-O1/Non-O139 V. cholerae. Various V. cholerae resistant phenotypes/genoytypes were detected vis: carbapenemase (CR-Vc; 31.1%/5.3%). New Delhi Metallobetalactamase (NDM-1-Vc; 23.0%/42.5%), extended spectrum betalactamase (ESBL-Vc; 42.6%/blaTEM:86,7%), chloramphenicol resistance (62.3%/Flor: 46.2%}, tetracycline resistance (70.5%/46.7%), AmpC resistance (21.0 (34.4%/56.7%)) and various other resistant genotypes/phenotypes. It was observed that more than 50% of the confirmed V. cholerae isolates possess resistance to two or more antibiotic classes/groups with multiple antibiotic resistance index (MARI) ranging from 0.031 to 0.5. This observation provides necessary information and updates for surveillance, planning and implementation of control strategies for cholera. It would also encourage decision making, formulation of policy by the government and cholera control authorities.

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Section: Discussionmentioning

confidence: 76%

Antibiotic Susceptibility Testing (AST) Reports: A Basis for Environmental/Epidemiological Surveillance and Infection Control Amongst Environmental Vibrio cholerae

Igere

Okoh

Nwodo

2020

IJERPH

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“…29 In this study, all the 370 V. cholerae isolates were absent for the toxic ctxAB and tcpA genes, consistent with our prior studies, 18,30,31 and some other literature. 22,[40][41][42][43] The ace, zot genes within the CTX element of pathogenic V. cholerae were also absent from all the isolates tested in this study. A similar observation was also reported based on non-O1/ O139 V. cholerae strains of environmental origins.…”

Section: Discussionmentioning

confidence: 99%

“…A similar observation was also reported based on non-O1/ O139 V. cholerae strains of environmental origins. 11,22,40,43 Recently, Olivares et al reported that non-O1/O139 V. cholerae, isolated from an 81-year-old woman with diarrheal disease, carried the rtxA gene. 44 Within the rtxCABD operon, the rtxA gene encodes an RTX toxin, whereas the rtxBD and rtxC genes encode two secretion proteins and a toxin activator, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…19 Resistant V. cholerae isolates that originated from clinical and environmental sources have been reported. 18,[20][21][22][23][24] For example, during an outbreak of cholera in Guinea-Bissau in 1996-1997, casefatality rates increased from 1% to 5.3% after the emergence of multidrug-resistant (MDR) V. cholerae. 20 Chomvarin et al reported that V. cholerae O1 strains (n = 35) isolated from clinical samples and natural surface water sources in Thailand were resistant to trimethoprim (TM)/sulfamethoxazole and/or tetracycline (TET)/or ampicillin (AMP).…”

Section: Introductionmentioning

confidence: 99%

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Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Pan

Liang

et al. 2020

Microbial Drug Resistance

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Vibrio cholerae is a waterborne bacterium and can cause epidemic cholera disease worldwide. Continuous monitoring of V. cholerae contamination in aquatic products is imperative for assuring food safety. In this study, we determined virulence, antimicrobial susceptibility, heavy metal tolerance, and genomic fingerprints of 370 V. cholerae isolates recovered from 12 species of commonly consumed aquatic products collected from July to September of 2018 in Shanghai, China. Among the species, Leiocassis longirostris, Ictalurus punetaus, Ophiocephalus argus Cantor, and Pelteobagrus fulvidraco were for the first time detected for V. cholerae. Toxin genes ctxAB, tcpA, ace, and zot were absent from all the V. cholerae isolates. However, high occurrence of virulence-associated genes was detected, such as hapA (82.7%), hlyA (81.4%), rtxCABD (81.4%, 24.3%, 80.3%, and 80.8%, respectively), and tlh (80.5%). Approximately 62.2% of the 370 V. cholerae isolates exhibited resistance to streptomycin, followed by ampicillin (60.3%), rifampicin (53.8%), trimethoprim (38.4%), and sulfamethoxazole-trimethoprim (37.0%). Moreover, *57.6% of the isolates showed multidrug resistant phenotypes with 57 resistance profiles, which was significantly different among the 12 species (multiple antimicrobial resistance index, p < 0.001). Meanwhile, high incidence of tolerance to heavy metals Hg 2+ (69.5%), Ni 2+ (32.4%), and Cd 2+ (30.8%) was observed among the isolates. The enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)-based fingerprinting profiles classified the 370 V. cholerae isolates into 239 different ERIC-genotypes, which demonstrated diverse genomic variation among the isolates. Overall, the results in this study meet the increasing need of food safety risk assessment of aquatic products.

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“…In Italy Halobacteriovorax and pathogenic vibrios, including V. parahaemolyticus and non-O1/O139 Vibrio cholerae , have been isolated in seawater and mussels coming from the Adriatic Sea ( Masini et al, 2007 ; Ottaviani et al, 2018a ). Moreover, in the last years, illness due to V. parahaemolyticus and non-O1/O139 V. cholerae , with mussels or seawater of Adriatic Sea as the source of infection, has been reported ( Ottaviani et al, 2010 , 2013 , 2018b ). In this work we studied the abundance and molecular diversity of V. parahaemolyticus -specific Halobacteriovorax strains isolated from seawater of a mussels growing area at the Conero Riviera of the Adriatic Sea.…”

Section: Introductionmentioning

confidence: 99%

Vibrio parahaemolyticus-specific Halobacteriovorax From Seawater of a Mussel Harvesting Area in the Adriatic Sea: Abundance, Diversity, Efficiency and Relationship With the Prey Natural Level

et al. 2020

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This research aimed to study the abundance and molecular diversity of Vibrio parahaemolyticus-specific Halobacteriovorax strains isolated from seawater of the Adriatic Sea and the relationship between predator and prey abundances. Moreover, predator efficiency of the Halobacteriovorax isolates toward V. parahaemolyticus and Vibrio cholerae non-O1/O139 strains was tested. V. parahaemolyticus NCTC 10885 was used as primary host for the isolation of Halobacteriovorax from seawater by the plaque assay. Molecular identification was performed by PCR detection of a fragment of the 16S rRNA gene of the Halobacteriovoraceae family members. Moreover, 700 bp PCR products were sequenced and compared between them and to clones described for other sampling sites. Vibrio counts were performed on TCBS agar from 100 ml of filtered water samples and presumptive colonies were confirmed by standard methods. Predatory efficiency of Halobacteriovorax isolates was tested by monitoring abilities of 3-day enrichments to form clear lytic halos on a lawn of Vibrio preys, by the plaque assay. Out of 12 seawater samples monthly collected from June 2017 to May 2018, 10 were positive for V. parahaemolyticus specific Halobacteriovorax with counts ranging from 4 to 1.4 × 10 3 PFU per 7.5 ml. No significant relationship was found between Halobacteriovorax and Vibrio abundances. The 16SrRNA sequences of our Halobacteriovorax strains, one for each positive sample, were divided into three lineages. Within the lineages, some sequences had 100% similarity. Sequence similarity between lineages was always <94.5% suggesting that they may therefore well belong to three different species. All Halobacteriovorax isolates had the ability to prey all tested

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Molecular characterization and drug susceptibility of non-O1/O139 V. cholerae strains of seafood, environmental and clinical origin, Italy

Cited by 23 publications

References 30 publications

Antibiotic Susceptibility Testing (AST) Reports: A Basis for Environmental/Epidemiological Surveillance and Infection Control Amongst Environmental Vibrio cholerae

Antibiotic Susceptibility Testing (AST) Reports: A Basis for Environmental/Epidemiological Surveillance and Infection Control Amongst Environmental Vibrio cholerae

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Vibrio parahaemolyticus-specific Halobacteriovorax From Seawater of a Mussel Harvesting Area in the Adriatic Sea: Abundance, Diversity, Efficiency and Relationship With the Prey Natural Level

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