Molecular characterization and tissue-specific expression of the acetyl-CoA carboxylase α gene from Grass carp, Ctenopharyngodon idella

Cheng, Hanliang; Ji, Nan-jing; Peng, Yong-xing; Shen, Xin; Xu, Jianhong; Dong, Zhiguo; Chen-chen, WU

doi:10.1016/j.gene.2011.07.027

Cited by 30 publications

(20 citation statements)

References 24 publications

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“…Thus, the highest expression level of accα in the liver and accβ in muscle observed in this study is consistent with that in mammals. Similarly, Cheng et al found that accα gene from grass carp was widely expressed in brain, mesenteric adipose, spleen, and white muscle, but highest in the liver. However, Zheng et al found that the highest mRNA levels of accα and accβ transcripts were detected in mesenteric adipose and heart of P. fulvidraco , respectively.…”

Section: Resultsmentioning

confidence: 89%

“…The current study extends our understanding of lipid metabolism in different tissues at the transcriptional level, which will facilitate further studies on the regulation of lipid metabolism at the molecular level for the fish species. Its production of malonyl-CoA is the committed step in the biosynthesis of long-chain fatty acids Lipogenesis [46] accb Its malonyl-CoA product inhibits the shuttle that transports long-chain acyl-CoAs from the cytosol to the mitochondria for oxidation Lipolysis [46] lpl Hydrolyze triglycerides in chylomicrons and very low-density lipoproteins for oxidation or storage Fatty acid transport [14] atgl Catalyze the initial step in triglyceride hydrolysis Lipolysis [49,50] At the end of acclimatization, fish were starved for 24 h before sampling. After being anesthetized with 3-aminobenzoic acid ethyl ester methanesulfonate (MS-222, 10 mg/L), nine fish (three fish for cDNA cloning and six fish for tissue mRNA levels analysis) were randomly selected and dissected on ice.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta

Chen

Luo

Huang

et al. 2014

Euro J Lipid Sci & Tech

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The regulation of lipid metabolism is complex, and is currently an extensive area of research. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned from the liver of Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, accα, accβ, lpl, atgl, hsla, hslb, cpt 1a, srebp‐1, pparα, and pparγ. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at varying levels. Practical applications: Excessive fat accumulation and disordered lipid metabolism have become serious problems in the sustainable and healthy development of aquaculture. The present study facilitates studies on the regulation of lipid metabolism at the molecular level in fish. The tissue expression profiles of genes increase our understanding of their physiological roles. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned and characterized from Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, accα, accβ, lpl, atgl, hsla, hslb, cpt 1a, srebp‐1, pparα, and pparγ. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at the varying levels.

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Section: Resultsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta

Chen

Luo

Huang

et al. 2014

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

show abstract

“…The ACC dimer hydration energy calculated by Franca et al [19] is −23.36 · 10 3 kJ/mol, and its isoelectric point is 6.23 [25]. As the hydration energy is small and electrostatic repulsion can be neglected, we assume that the AFM tip surface is uniformly covered by ACC dimers represented by rigid polyhedrons.…”

Section: Number Of Functionalized Enzymes On the Afm Tip Surfacementioning

confidence: 99%

Modeling the coverage of an AFM tip by enzymes and its application in nanobiosensors

Amarante

Oliveira

Bueno

et al. 2014

Journal of Molecular Graphics and Modelling

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“…Some diseases associated with lipid metabolism, such as fatty liver syndrome, have been found to increase year after year in cultured grass carp (Cheng, Ji, Peng, Shen, Xu, Dong & Wu 2011). Some diseases associated with lipid metabolism, such as fatty liver syndrome, have been found to increase year after year in cultured grass carp (Cheng, Ji, Peng, Shen, Xu, Dong & Wu 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of waterborne copper on lipid metabolism in hepatopancreas and muscle of grass carpCtenopharyngodon idella

Mai

Luo³

et al. 2016

Aquac Res

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This study aims to determine the effect of waterborne copper (Cu) exposure on lipid metabolism in the hepatopancreas and muscle of grass carp Ctenopharyngodon idella. Grass carp was exposed to four levels of waterborne Cu (control, 0.058, 0.150 and 0.199 mg L À1 ) for 14 days. The lipid content, Cu accumulation, and the activities and expression levels of several enzymes involved in lipid metabolism were determined in hepatopancreas and muscle. Copper (Cu) accumulation was found in both tested tissues and the content increased with the increase in waterborne Cu concentrations. Waterborne Cu exposure reduced the lipid content and growth performance. In hepatopancreas and muscle, the activities and the mRNA expression of lipogenic enzymes (G6PD, 6PGD, ICDH, ME and FAS) decreased with the increase of waterborne Cu levels. Furthermore, the mRNA expression levels of lipolytic enzymes (HSL and ATGL) and transcription factors (SREBP-1 and PPARa) were downregulated by Cu exposure. The decrease of lipogenesis prevailed over that of lipolysis, which might lead the reduction in lipid contents. Taken together, this study demonstrates that waterborne Cu exposure can perturb the normal processes of lipid metabolism, which serves to improve our understanding of the mechanisms underlying lipid metabolism and deposition during Cu exposure in fish.

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Molecular characterization and tissue-specific expression of the acetyl-CoA carboxylase α gene from Grass carp, Ctenopharyngodon idella

Cited by 30 publications

References 24 publications

Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta

Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta

Modeling the coverage of an AFM tip by enzymes and its application in nanobiosensors

Effect of waterborne copper on lipid metabolism in hepatopancreas and muscle of grass carpCtenopharyngodon idella

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