Molecular characterization, expression and functional analysis of peptidoglycan recognition protein-SC2 from rock bream, Oplegnathus fasciatus

Choi, Kwang-Min; Joo, Min-Soo; Cho, Dong-Hee; Bae, Jin-Sol; Jeong, Ji-Min; Woo, Won-Sik; Han, Hyun‐Ja; Lee, Deok Chan; Cho, Mi Young; Jung, Sung Hee; Kim, Do-Hyung; Park, Chan-Il

doi:10.1016/j.fsi.2018.04.006

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…The multiple alignment of the PmSCAMP5 amino acid sequence and the SCAMP5 amino acid sequence of other species registered in peptide sequence database of NCBI was analysed using ClustalW analysis. The phylogenetic analysis of PmSCAMP5 was performed using the neighbour-joining (NJ) method of MEGA 4 program and bootstrap sampling was repeated 2,000 times [1].…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Data on molecular characterization and gene expression analysis of secretory carrier-associated membrane protein 5 (SCAMP5) from the red sea bream (Pagrus major)

2019

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Secretory carrier membrane proteins (SCAMPs) are widely distributed integral membrane proteins implicated in membrane trafficking. Secretory carrier membrane protein 5 (SCAMP5) is expected to be involved in regulation of the immune response because it is expressed in a variety of immune tissues and promotes the secretion of cytokines in monocytes and macrophages. In this study, we performed an analysis of the molecular characteristics and phylogenetic of the SCAMP5 gene identified in Pagrus major (PmSCAMP5). In addition, we analysed PmSCAMP5 gene expression levels in the tissues of red sea bream infected with various pathogens [ Edwardsiella piscicida ( E. piscicida ), Streptococcus iniae ( S. iniae ) and Red sea bream iridovirus (RSIV)], and we analysed PmSCAMP5 gene expression levels in the tissues of healthy red sea bream. This study was carried out to provide basic data on the non-specific immune system of the red sea bream.

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…4). The results are reported as the mean ± standard deviation (SD) and were assessed using one-way analysis of variance (ANOVA) followed by Duncan's test (* P value < 0.05 and ** P value < 0.01) in SPSS software 19.0 (IBM, USA) [1].…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Data on molecular characterization and gene expression analysis of secretory carrier-associated membrane protein 5 (SCAMP5) from the red sea bream (Pagrus major)

2019

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“…Eight kinds of bacteria used in the bacteria binding assay were also selected for this test as described previously (Choi et al, 2018). Microorganisms in the logarithmic phase were resuspended with TBS to 3*10 6 CFU/mL.…”

Section: Microbial Agglutination Assay Of Hopgrp-l2mentioning

confidence: 99%

“…PGRP2 from Ctenopharyngodon idella was characterized as having the ability to speci cally bind PGN and possessing an amidase activity (J. H. Li, Chang, Xue, & Nie, 2013). PGRP2 from Oncorhynchus mykiss was demonstrated to modulate the body's in ammatory response by reducing NF-κB activity during bacterial infection (Choi et al, 2018). In Lateolabrax maculatus, Ssb-PGRP-L2 expressed bactericidal effects on Vibrio harveyi, Staphylococcus aureus, and Edwardsiella tarda (X.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and functional analysis of Peptidoglycan recognition protein-L2 in Hexagrammos otakii

Bai,

Liu

2024

Preprint

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Peptidoglycan recognition proteins (PGRPs) belong to the pattern recognition receptors (PRRs), which are essential for sensing and defending against pathogenic microorganisms during innate immunity pathways. Although an increasing body of research indicates that the PGRP protein in fish has various biological functions such as antimicrobial activity, amidase activity, and the ability to regulate multiple signaling pathways, the molecular mechanisms by which PGRP contributes to the innate immune processes in fish remain relatively limited. In the present study, we have recombinantly expressed a long-type PGRP from fat greenling (Hexagrammos otakii) (HoPGRP-L2) and analyzed its molecular mechanism in the pathogen identification process. The open reading frame (ORF) of HoPGRP-L2 is 1449 bp in length that encodes for a peptide with 482 amino acids. As a PRR, HoPGRP-L2 has a typical PGRP domain that enables HoPGRP-L2 to recognize and conjugate to bacterial peptidoglycan (PGN) on the cell wall. We demonstrated that HoPGRP-L2 could bind to pathogenic microorganisms and promote the agglutination of them. Furthermore, HoPGRP-L2 was confirmed to possess zinc ion-dependent amidase activity and exhibited an effect on the growth inhibition of chosen bacteria. HoPGRP-L2 also prolongs the survival time in carp injected with Aeromonas hydrophila. Taken together, our results indicate that PGRP acts as a PRR involved in recognizing and eliminating pathogens during the innate immune response in Hexagrammos otakii.

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“…Among fish species, PGRP molecules were found in zebra fish ( Danio rerio ) [16, 17], rockfish ( Sebastes schlegeli ) [18], rock bream ( Oplegnathus fasciatus ) [19], large yellow croaker ( Pseudosciaena crocea ) [20], channel catfish ( Ictalurus punctatus ) [21], green-spotted pufferfish ( Tetraodon nigroviridis ) [16], grass carp ( Ctenopharyngodon idella ) [11, 22, 23], rainbow trout ( Oncorhynchus mykiss ) [24, 25], turbot( Scophthalmus maximus ) [26], tongue sole ( Cynoglossus semilaevis ) [27], and red drum ( Sciaenops ocellatus ) [28]. The sequence of PGRP5 and PGRP6 gene are also cloned from common carp ( Cyprinus carpio ).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression analysis of two peptidoglycan recognition proteins (CcPGRP5, CcPGRP6) in larvae ontogeny of common carp Cyprinus carpio L. and upon immune stimulation by bacteria

Zhang

Shan

et al. 2019

BMC Vet Res

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BackgroundAlthough teleost fish developed acquired immunity firstly in evolution, innate immunity is still very important for them. Innate immunity depends on pattern recognition receptors (PRRs) to distinguish “self” and “non-self”, Peptidoglycan (PGN) recognition protein (PGRP) is one of the receptors and it can bind to multiple components of bacterial envelope.ResultsWe report the cloning and expression analysis of two PGRPs (Ccpgrp5 and Ccpgrp6) from common carp (Cyprinus carpio L). The Ccpgrp5 gene encodes a protein of 199 amino acid (aa) with PGRP domain, Ami_2 domain and four Zn2+ binding sites required for amidase activity, but without signal peptide and transmembrane domain. The Ccpgrp6 gene encodes a protein of 446 aa with PGRP domain, Ami_2 domain, signal peptide, five Zn2+ binding sites required for amidase activity and two sites for N-glycosylation. The phylogenetic analysis revealed that the CcPGRP5 and CcPGRP6 are closely related to Ctenopharyngodon idella and Danio rerio. Ccpgrp5 and Ccpgrp6 were expressed in all tissues examined including liver, spleen, muscle, oral epithelium, head kidney, gill, skin, gonad, brain, foregut and hindgut and showed different distribution characteristics. During the embryonic and early larval developmental stages of common carp, Ccpgrp6 was detected to be highly expressed at 10 days post fertilization(dpf) and 36 dpf, while Ccpgrp5 were hardly detected using Real-time quantitative PCR. After being challenged with Aeromonas hydrophila, Ccpgrp5 in adult common carp was induced and up-regulated in all the tissues, especially in gill and spleen, but not in head kidney, while Ccpgrp6 was up-regulated in all the tissues, especially in liver, head kidney and gill. The varied expression profiling of Ccpgrp5 and Ccpgrp6 indicated they had different roles in the host immune response.ConclusionsThese results indicated the two PGRPs, especially Ccpgrp6, played an important role in the immune defense of common carp during larva development and against Aeromonas hydrophila, providing insight to further exploration of protecting fish against bacteria infectious disease.

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Molecular characterization, expression and functional analysis of peptidoglycan recognition protein-SC2 from rock bream, Oplegnathus fasciatus

Cited by 10 publications

References 33 publications

Data on molecular characterization and gene expression analysis of secretory carrier-associated membrane protein 5 (SCAMP5) from the red sea bream (Pagrus major)

Data on molecular characterization and gene expression analysis of secretory carrier-associated membrane protein 5 (SCAMP5) from the red sea bream (Pagrus major)

Molecular characterization and functional analysis of Peptidoglycan recognition protein-L2 in Hexagrammos otakii

Molecular characterization and expression analysis of two peptidoglycan recognition proteins (CcPGRP5, CcPGRP6) in larvae ontogeny of common carp Cyprinus carpio L. and upon immune stimulation by bacteria

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