Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea

Kim, Jin Eui; Lee, Young ju

doi:10.1186/s13620-017-0095-8

Cited by 34 publications

(32 citation statements)

References 37 publications

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“…The serotype Paratyphi B was grouped into cluster 6, but some strains were also not grouped in a cluster; these results are in agreement with those reported by Kim and Lee [ 55 ] who described that ERIC-PCR can be used to sub-typify within the serotype. In addition, S .…”

Section: Discussionsupporting

confidence: 91%

“…[ 54 ] who found a discrimination index of 0.70 but lower than those reported by Fendri et al . [ 53 ] and Kim and Lee [ 55 ] who found discriminatory indexes of 0.85 and 0.97, respectively. However, in the present study, the ERIC-PCR method was able to discriminate the strains according to their source of isolation, and a greater discriminatory power does not always represent a grouping with biological significance [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Finally, in the present study, no relationship was found between the clusters and the antibiotic resistance. Kim and Lee [ 55 ] and Oliveira et al . [ 7 ] also reported similar results in which the clusters did not exhibit the same pattern of antibiotic resistance.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

2020

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Background and Aim: Salmonella spp. are one of the most important food-borne pathogens in the world, emerging as a major public health concern. Moreover, multidrug-resistant (MDR) strains have been isolated from salmonellosis outbreaks, which compromise its treatment success. This study was conducted to characterize the phenotypic and genotypic antibiotic resistance profile of Salmonella strains isolated from broilers and humans from the regions of Tolima and Santander (Colombia). Materials and Methods: Salmonella spp. strains (n=49) were confirmed through molecular detection by amplification of the invA gene. Phenotypic antibiotic resistance was determined by the automated method and the agar diffusion method, and the presence of resistance genes was evaluated by PCR. Genotypic characterization was conducted using the enterobacterial repetitive intergenic consensus (ERIC)-PCR method, from which a dendrogram was generated and the possible phylogenetic relationships were established. Results: Salmonella isolates were classified as MDR strains exhibiting resistance to four antibiotic classes, penicillins, aminoglycosides, sulfonamides, and cephalosporins, and the human strains were resistant to gentamicin. At the genotypic level, the isolates contained the genes blaCMY2, blaCTX-M, blaPSE-1, blaTEM, aadA1, srtB, dfrA1, sul2, and floR. The genotyping results obtained by ERIC-PCR allowed the grouping of strains according to the source of isolation. Conclusion: The Salmonella spp. strains exhibited resistance to multiple antibiotics, as well as multiple genes associated with them, and the ERIC-PCR method was a technique that was helpful in generating clusters with biological significance.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

2020

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“…ERIC-PCR fingerprinting has also been used to confirm epidemiological relationships between various Salmonella isolates . In Korea, researchers claimed that ERIC-PCR combined with virulence profiling offered a rapid approach to characterize antimicrobial-resistant Salmonella (Jin & Lee, 2017). We did not find a significant association between ERIC-PCR clusters, source, and other characteristics.…”

Section: Discussioncontrasting

confidence: 80%

Antimicrobial resistance and genetic diversity of Salmonella enterica from eggs

Xie

et al. 2019

Food Science & Nutrition

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Salmonella enterica is a common foodborne pathogen responsible for major global health problems such as paratyphoid fever and gastroenteritis. Here, we report the prevalence, antibiotic resistance phenotypes, serotypes, and molecular subtyping of Salmonella isolated from eggs in Guangdong, China. Out of 1,000 egg samples, 54 (5.40%) were positive. S. Enteritidis made up the largest proportion of samples with 11 serotypes. Antimicrobial susceptibility test indicated that most strains were resistant to β‐lactam, aminoglycoside, and tetracycline antibiotics (27.00%–40.00%). There were 37 STs based on MLST typing. MLST and ERIC‐PCR classified 54 isolates into three and five clusters, respectively, which revealed the genetic relatedness and diversity. In conclusion, frequent monitoring of eggs for Salmonella, antibiotic resistance profiles and genetic diversity is essential for improving food safety.

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“…Các gen độc lực của Salmonella có mặt ở nhiễm sắc thể, plasmid và tiền thực khuẩn thể của vi khuẩn, Salmonella gây nhiễm trùng nhiễm độc thực phẩm (SPIs) đóng vai trò quan trọng trong việc bám dính, xâm nhiễm, sống sót nội bào, nhiễm trùng hệ thống, kháng kháng sinh, tạo độc tố và hấp thu Mg và Fe (Kim, Ju Lee, 2017). Các gen mục tiêu invA, orgA, prgH, spaN (invJ), tolC, sipB, pagC, msgA, spiA, sopB, lpfC, pefA, spvB và sifA mã hóa cho các protein liên quan đến tính xâm nhiễm, như xâm nhiễm tế bào và sự bám dính hoặc tạo ra nhung mao (Skyberg et al, 2006).…”

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The survey of expression levels of virulent genes in Salmonella strains isolated in retail meats in Hanoi

Thu

Viet

Minh

et al. 2018

V J Bio

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Salmonella is one of the major causes of food poisoning worldwide, especially in developing countries. The virulence of Salmonella is a complex process involving between their virulence factors and the host defenses. The purpose of this study was to detect virulent genes and to assess the level of expression of these genes in five Salmonella strains (including Enteritidis, Albany, Typhimurium, Hadar, and Derby), which were isolated from meat samples at the retail markets in Hanoi. As a result, Salmonella Enteritidis and Salmonella Typhimurium were 100% positive with seven virulent genes, e.g., spiA, spvB, sitC, sifA, sipB, pagC, and invA genes. Salmonella Hadar and Salmonella Derby positive with six genes, except spvB, whereas Salmonella Albany was positive with only the pagC gene. The comparision of the expression levels of these seven virulent genes and the 16S rRNA control gene showed a significantly difference among Salmonella strains (P<0.05). The result of gene expression of seven virulent genes indicate that Salmonella Hadar has the highest gene expression, followed by Salmonella Enteritidis, Salmonella Derby, Salmonella Typhimurium and finally Salmonella Albany. The results of molecular biology analysis will provide additional data on the expression of virulent genes in Salmonella strains isolated from retail meats in Hanoi.

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Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea

Cited by 34 publications

References 37 publications

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

Antimicrobial resistance and genetic diversity of Salmonella enterica from eggs

The survey of expression levels of virulent genes in Salmonella strains isolated in retail meats in Hanoi

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