Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting

Abstract

Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected.

Differentiation of citrus bacterial canker strains in Korea by host range, rep-PCR fingerprinting and 16S rDNA analysis

“…Our results of rep-PCR also indicated that A* and A w types were close to A types, but they were excluded from A types in this combined analysis. Trindade et al (2005) argued that dendrograms obtained with REP, ERIC Fig. 1 Dendrogram showing relationships among Xanthomonas strains causing CBC based on combined analysis of ERIC-, BOX-and REP-PCR results.…”

Genetic Diversity of Xanthomonas Citri Subsp. Citri, Causal Agent of Citrus Canker

“…Only the reproducible bands ranging from 0.2 to 3 kb were scored. Since combined analysis of rep-PCR primers showed a significant increase in differentiation capacity in other works (Trindade et al 2005;Lee et al 2007), combined data generated from two sets of primers were combined and then subjected to analysis. To estimate the genetic relationship among the strains, the similarity coefficient for pairs of isolates was calculated with Dice's coefficient index (Dice 1945).…”

Genetic diversity of the common bacterial blight pathogen of bean, Xanthomonas axonopodis pv. phaseoli, in Iran revealed by rep-PCR and PCR–RFLP analyses

“…d Sau96I: Lane M is 50 bp molecular size marker, Lane 1 is uncut product, Lane 2 is Xp21 (Xapf), Lanes 3-10 are Xp7, Xp17, Xp12, Xp11, Xp18, Xp2, Xp13 and Xp20, Lane 11 is negative control World J Microbiol Biotechnol (2011) 27:2371-23782375 polymorphism among Brazilian X. campestris pv. viticola strains and allowed their separation into five subgroups, although no correlation could be found with cultivar, geographic location or year of origin (Trindade et al 2005).…”