Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014–2017)

Falcão, Marcus Vinícius Dias; Laroucau, Karine; Vorimore, Fabien; Deshayes, Thomas; Santana, Vania Lucia Assis; Silva, Karla P. C; Nascimento, Sérgio Alves do; Castro, Roberto Soares de; Araújo, Flábio R.; Mota, Rinaldo Aparecido

doi:10.1016/j.meegid.2022.105250

Cited by 8 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Few studies employed WGS-based SNP typing to B. mallei outbreak analysis. The agreement of SNP analysis with conventional VNTR analysis and HRM-PCR was proven for regional glanders outbreak events (40,63), attesting the accordance of both methods on a small scale. Laroucau et al (41) provided one of the first SNP-based analysis of the global B. mallei population.…”

Section: Whole Genome Sequencing-based Typingmentioning

confidence: 64%

“…Application of HRM-PCR for B. mallei typing was first introduced by Girault et al ( 62 ) who described a set of 15 SNPs specific for each of the three phylogenetic clusters identified by Laroucau et al ( 41 ). The HRM-PCR using this set of SNPs was validated with recently isolated B. mallei strains from India, Pakistan and Brazil ( 45 , 62 , 63 ) allowing further differentiation within the clusters. Recent B. mallei isolates from Dubai, UAE, Bahrain clustered in L2B1, strains from India and Pakistan clustered in L2B2sB2 whereas strains from Brazil clustered in L3B2.…”

Section: B Mallei Diversitymentioning

confidence: 99%

See 1 more Smart Citation

Sequence-based detection and typing procedures for Burkholderia mallei: Assessment and prospects

Brangsch

Singha²,

Laroucau³

et al. 2022

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

Although glanders has been eradicated in most of the developed world, the disease still persists in various countries such as Brazil, India, Pakistan, Bangladesh, Nepal, Iran, Bahrain, UAE and Turkey. It is one of the notifiable diseases listed by the World Organization for Animal Health. Occurrence of glanders imposes restriction on equestrian events and restricts equine movement, thus causing economic losses to equine industry. The genetic diversity and global distribution of the causing agent, Burkholderia (B.) mallei, have not been assessed in detail and are complicated by the high clonality of this organism. Among the identification and typing methods, PCR-based methods for distinguishing B. mallei from its close relative B. pseudomallei as well as genotyping using tandem repeat regions (MLVA) are established. The advent and continuous advancement of the sequencing techniques and the reconstruction of closed genomes enable the development of genome guided epidemiological tools. For achieving a higher genomic resolution, genotyping methods based on whole genome sequencing data can be employed, like genome-wide single nucleotide polymorphisms. One of the limitations in obtaining complete genomic sequences for further molecular characterization of B. mallei is its high GC content. In this review, we aim to provide an overview of the widely used detection and typing methods for B. mallei and illustrate gaps that still require development. The genomic features of Burkholderia, their high homology and clonality will be first described from a comparative genomics perspective. Then, the commonly used molecular detection (PCR systems) and typing systems (e.g., multilocus sequence typing, variable number of tandem repeat analysis) will be presented and put in perspective with recently developed genomic methods. Also, the increasing availability of B. mallei genomic sequences and evolution of the sequencing methods offers exciting prospects for further refinement of B. mallei typing, that could overcome the difficulties presently encountered with this particular bacterium.

show abstract

Section: Whole Genome Sequencing-based Typingmentioning

confidence: 64%

Section: B Mallei Diversitymentioning

confidence: 99%

Sequence-based detection and typing procedures for Burkholderia mallei: Assessment and prospects

Brangsch

Singha²,

Laroucau³

et al. 2022

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to Falcão et al (2022) [38], the L3B2 lineage in Brazil consists of several isolates of B. mallei, including 9902 RSC, BM_campo 1, BM_campo 3, and UFAL2, which were obtained from the Northeast region of Brazil. Laroucau et al (2018) also confirmed that the 16-2438_BM#8 strain, collected from the same region, is part of this lineage.…”

Section: Discussionmentioning

confidence: 99%

“…The L3B2 lineage in Brazil includes isolates that have caused either clinical infections (such as UFAL 2 and 16-2438_BM#8) or asymptomatic infections (such as BAC 86/19, 9902 RSC, BM_campo 1, and BM_campo 3) [38,39].…”

Section: Discussionmentioning

confidence: 99%

Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing

Suniga,

Mantovani,

dos Santos

et al. 2023

Pathogens

View full text Add to dashboard Cite

This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders.

show abstract

Current status of glanders in Brazil: recent advances and challenges

Mota

Pinheiro²

2022

Braz J Microbiol

View full text Add to dashboard Cite

Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014–2017)

Cited by 8 publications

References 15 publications

Sequence-based detection and typing procedures for Burkholderia mallei: Assessment and prospects

Sequence-based detection and typing procedures for Burkholderia mallei: Assessment and prospects

Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing

Current status of glanders in Brazil: recent advances and challenges

Contact Info

Product

Resources

About