Molecular characterization of Cryptosporidium spp. in native breeds of cattle in Kaduna State, Nigeria

Maikai, B.V.; Umoh, J.U.; Kwaga, J. K. P.; Lawal, Idris Alao; Maikai, VA; Cama, Vitaliano; Xiao, Lihua

doi:10.1016/j.vetpar.2010.12.048

Cited by 64 publications

(51 citation statements)

References 29 publications

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“…Of 12 C. parvum positive fecal samples, 8 were from diarrheic calves and 4 from calves with loose feces (Table 1). In disagreement with some previous studies [35,39,41,42] , our results proved an association of fecal consistency with the infection. Studies reporting relationship between fecal consistency and cryptosporidiosis are available [7,12,36] .…”

Section: Discussioncontrasting

confidence: 99%

Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı ve Cryptosporidium Türlerinin Moleküler Karekterizasyonu

Güven¹,

Avcıoğlu²,

Balkaya³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryThis study was conducted to determine the prevalence of cryptosporidiosis and to identify Cryptosporidium species found in preweaned calves, in Erzurum, Turkey. Fecal samples were collected from 307 calves up to one month old from 5 dairy farms. Genomic DNA was obtained by DNA extraction (QIAamp DNA Stool kit). The prevalence of cryptosporidiosis was determined based on identification through a nested PCR protocol to amplify fragments of the Cryptosporidium SSU rRNA gene. 3.9% of calves were positive for Cryptosporidium. Calves that were subjected to traditional herd management, were female, aged 2 weeks, and had watery feces were affected by the disease at a greater incidence than those were subjected to planned herd surveillance program, were males, were older than 3 weeks, and had firm feces. DNA sequence analysis of the SSU rRNA gene on all of the PCR positive samples ascertained that C. parvum was the only species present. Further studies should be performed comprehensive fecal analysis for other causative agents for association Cryptosporidium species in calf diarrhea and mortality resulting in economic loss in the region. Keywords: Calf, Cryptosporidium, Nested-PCR, SSU rRNA, Erzurum Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı veCryptosporidium Türlerinin Moleküler Karekterizasyonu ÖzetBu çalışma, Erzurum yöresindeki sütten kesim öncesi dönemdeki buzağılarda cryptosporidiosisin prevalansının ve Cryptosporidium türlerinin moleküler karekterizasyonunun ortaya konması amacıyla yapılmıştır. Bu amaçla beş süt işletmesinden, bir aydan küçük 307 buzağının dışkı örnekleri toplanmış ve DNA ekstraksiyonu (QIAamp DNA Stool kit) yapılarak genomik DNA elde edilmiştir. Nested PCR protokolü ile Cryptosporidium SSU rRNA gen bölgesinin kısmi amplifikasyonu yapılmış ve cryptosporidiosis prevalansı %3.9 olarak belirlenmiştir. Geleneksel yöntemlerle yetiştirilen, dişi, 2 haftalık yaşta ve sulu dışkıya sahip buzağıların modern işletmelerde yetiştirilen, erkek, 3 haftadan büyük ve katı kıvamlı dışkıya sahip olanlara göre hastalıktan daha çok etkilendiği saptanmıştır. PCR pozitif örneklerin SSU rRNA gen bölgesi hedef alınarak yapılan DNA dizi analizleri sonuçlarına göre C. parvum'un hayvanlarda bulunan tek tür olduğu anlaşılmıştır. Sonuç olarak, yörede ekonomik kayıplara neden olan buzağı ishalleri ve ölümlerinde Cryptosporidium türleri ile diğer hastalık etkenlerinin etkileşimlerinin ortaya konması amacıyla daha kapsamlı çalışmaların yapılmasının gerekliliği sonucuna varılmıştır.

show abstract

Section: Discussioncontrasting

confidence: 99%

Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı ve Cryptosporidium Türlerinin Moleküler Karekterizasyonu

Güven¹,

Avcıoğlu²,

Balkaya³

et al. 2013

Kafkas Univ Vet Fak Derg

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“…However, this species was not detected in the present study. The absence of C. parvum has been shown in native breeds of cattle in developing countries (Maikai et al, 2011;Feng et al, 2012;Nguyen et al, 2012). However, in industrialized nations, beef calves raised under traditional animal husbandry do not usually have C. parvum (Feltus et al, 2008;Fayer et al, 2010;Murakoshi et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Cryptosporidium spp. in pre-weaned calves in Shaanxi Province, north-western China

Fang

Wang

et al. 2015

Journal of Medical Microbiology

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“…Indeed, high temperature and increased solar radiation has been demonstrated to enhance Cryptosporidium oocyst inactivation in the environment (Fujino et al, 2002). Intriguingly, two recent studies from West Africa (Nigeria) have demonstrated the occurrence of Cryptosporidium infection in Nigerian cattle herds, but only C. bovis, C. ryanae and C. andersoni were found, and C. parvum was not detected (Ayinmode et al, 2010;Maikai et al, 2011). As almost all environmental survival studies have been conducted on oocysts of C. parvum, it is possible that oocysts from other species of Cryptosporidium are better able to tolerate dry, warm conditions.…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that pure B. p. taurus cattle may be more susceptible to Cryptosporidium infection. Indeed, it has previously been suggested that some B. p. indicus breeds may be more resistant, although the data are scanty (Maikai et al, 2011). However, other studies have reported Cryptosporidium infection from both B. p. indicus and crossbreed cattle (Swai and Schoonmann, 2010).…”

Section: Discussionmentioning

confidence: 99%

Unexpected results from large-scale cryptosporidiosis screening study in calves in Tanzania

Chang’a

Robertson

Mtambo

et al. 2011

Annals of Tropical Medicine & Parasitology

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A study was undertaken to investigate Cryptosporidium infection in crossbreed dairy calves in two districts in Tanzania. A total of 943 fecal samples from 601 dairy calves were included in the study, with calves from both smallholder dairy farms and from large-scale and medium-scale dairy farms. The modified Ziehl-Neelsen (mZN) technique was used to examine 710 samples, and 13 of these were considered to be positive for Cryptosporidium. These 13 samples considered positive by mZN, along with the remaining 233 samples, were analysed by immunofluorescent antibody test (IFAT). Of these 246 samples examined by IFAT, 15 samples, 10 of which were considered positive by mZN, were also examined by the auramine phenol technique, and 5 samples, all of which were considered positive by mZN, were analysed by PCR. The results from the IFAT, auramine phenol and PCR analyses demonstrated that none of the samples contained Cryptosporidium oocysts, indicating that, cryptosporidiosis is currently not a problem in dairy calves in these regions of Tanzania. These unexpected results are discussed with respect to other reports on cryptosporidiosis in calves that suggest that this parasite is a serious calf disease globally, and particularly in relation to studies from Tanzania. We suggest that results from studies of cattle in Tanzania, in which mZN has been used as the sole analytical method, should be treated with caution.

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Molecular characterization of Cryptosporidium spp. in native breeds of cattle in Kaduna State, Nigeria

Cited by 64 publications

References 29 publications

Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı ve Cryptosporidium Türlerinin Moleküler Karekterizasyonu

Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı ve Cryptosporidium Türlerinin Moleküler Karekterizasyonu

Molecular characterization of Cryptosporidium spp. in pre-weaned calves in Shaanxi Province, north-western China

Unexpected results from large-scale cryptosporidiosis screening study in calves in Tanzania

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