Molecular characterization of Colletotrichum species causing soybean anthracnose in Argentina

Abstract

-Twenty-six isolates obtained from soybean crops (Glycine max) with typical anthracnose symptoms were identified as Colletotrichum truncatum (73 %) and C. destructivum (26 %). Their genetic relationships were studied using the AFLP method. A UPGMA phenogram divided the strains into two clusters corresponding with the two species. Genetic distances based on association coefficient were 0.71-0.89 among the 18 C. truncatum strains and 0.67-1 among the eight C. destructivum strains. Genetic variability within species, measured in terms of percentage of polymorphic loci, was high (<90%). Only two isolates showed 100% similarity, suggesting high intraspecific variability.

Phylogeny and variability ofColletotrichum truncatumassociated with soybean anthracnose in Brazil

“…Formerly associated exclusively to C. truncatum, soybean anthracnose has been gradually associated with other Colletotrichum species, such as C. coccodes, C. gloeosporioides, C. destructivum, C. chlorophyti and C. incanum (Riccioni et al 1998;Chen et al 2006;Hang 2013;Mahmodi et al 2013;Ramos et al 2013;Yang et al 2014). In Brazil, it is assumed that the disease is associated with C. truncatum; however, studies that investigated the disease aetiology and its possible genetic variation are scarce.…”

“…), Colletotrichum destructivum (Ramos et al . ), Colletotrichum chlorophyti (Hang ) and, more recently, Colletotrichum incanum (Yang et al . ).…”

“…In Brazil, anthracnose was first detected in 1961 in Rio Grande do Sul (EMBRAPA 2008). Other Colletotrichum species have also been reported as causal agents of diseases in soybean in other countries, such as Colletotrichum coccodes (Riccioni et al 1998), Colletotrichum gloeosporioides (Chen et al 2006;Mahmodi et al 2013), Colletotrichum destructivum (Ramos et al 2013), Colletotrichum chlorophyti (Hang 2013) and, more recently, Colletotrichum incanum (Yang et al 2014).…”

Enfermedades de fin de ciclo y mancha ojo de rana en el cultivo de soja: desarrollo de un sistema de puntuación y determinación del umbral de control

“…En un trabajo del año 2013, se realizó la caracterización molecular de las especies de Colletotrichum causantes de antracnosis de la soja en Argentina (Ramos et al, 2013). De acuerdo con estos autores, Colletotrichum truncatum fue identificado en el 73 % de los aislados y Glomerella glycines en el 26 %.…”

“…Hill, 2010;Marinelli et al, 2011;, su ocurrencia en los tejidos foliares demuestra que los mismos pueden también estar infectando los foliolos de soja. En relación a los peritecios de C. destructivum, (G. glycines) su presencia es frecuente en los campos de Argentina (Carmona et al, 2006;Ramos et al, 2013).…”