Molecular characterization ofRhizoctonia solaniisolates the incitant of soybean root rot

Abstract

Rhizoctonia solani isolates used in this investigation were identified as anastomosis-4 (AG-40), collected from different localities from Assiut governorate in Egypt. Pathogenicity test of seven isolates of R. solani was evaluated on soybean Giza 111 cultivar under greenhouse conditions. All tested isolates were able to infect soybean plants causing root rot with different degrees of severities, isolate No. 1, 2 and 3 showed significantly highest root rot severity, while isolate No. 5 gave the lowest percentage of root rot rating. The sodium dodecyl sulphate polyacrylamide gel electrophoresis patterns were used to compare three isolates of R. solani. There are no variations among R. solani isolates except a few exceptions according to their protein patterns. DNA markers obtained from all isolates showed genetic similarity among different isolates obtained from different geographical regions barring few exceptions. Correlation between DNA patterns of R. solani isolates and their virulence was detected, but no correlation with anastomosis groups (AG).

Endophytic fungi associated with soybean plants and their antagonistic activity against Rhizoctonia solani

“…The plates were incubated for 7 days at 28 °C. When the growth of the pathogen was covered, the plate in control treatment in the linear growth of the tested pathogen was recorded in the treatments fungi (Zein El-Abdean et al 2013). The percentage of mycelial growth inhibition was calculated using the following formula: percentage of mycelial growth inhibition = [T-F/T] × 100, where T is the mycelia growth in the control and F is the mycelia growth in the tested isolate.…”

Next-generation sequencing of root fungal communities in continuous cropping soybean

“…under continuous cropping in Northeast China (Zhang et al, 2011;Liu et al, 2012). This disease is extremely harmful and difficult to prevent and control (Hartman et al, 2011;El-Abdean et al, 2013). In the early stages of plant development, it affects seedling growth and can even cause their death.…”

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“…It is generally difficult to predict the pathogenic behavior of a race and its isolates because it depends on the variations in the environmental conditions, the aggressiveness of the pathogenic race or anastomotic group as well as the host plant growth conditions (Ristaino & Johnston, 1999). Therefore, it is necessary to evaluate the aggressiveness of P. capsici (Gil-Ortega et al, 1995;Black & Berke, 1998;Oelke et al, 2003;Glosier et al, 2008;Sy et al 2008) and R. solani (Muhyi & Bosland, 1995;El-Abdean et al, 2013) by considering the different geographical origins of the isolates from each species. Thus, the pathogenicity and incidence of different Phytophthora capsici L. and Rhizoctonia solani isolates were evaluated in 'costeño' pepper (Capsicum annuum L.) seedlings in Oaxaca, Mexico.…”

“…En general, es difícil predecir el comportamiento patogénico de una raza y sus asilamientos, este depende de la consecuencia de variación de las condiciones ambientales, agresividad de la raza patogénica o grupo anastomótico y condiciones de crecimiento de la planta huésped (Ristaino & Johnston, 1999). Por tanto, es necesario evaluar la agresividad de P. capsici (Gil-Ortega et al, 1995;Black & Berke, 1998;Oelke et al, 2003;Glosier et al, 2008;Sy et al, 2008) y R. solani (Muhyi & Bosland, 1995;El-Abdean et al, 2013) considerándose diferentes orígenes geográficos de los aislamientos de cada especie. En este sentido, se evaluó la patogenicidad e incidencia de diferentes aislamientos de Phytophthora capsici L. y Rhizoctonia solani en plántulas de chile costeño (Capsicum annuum L.) en Oaxaca, México.…”