Molecular characterization of Mycobacterium tuberculosis isolates from Łódź, Poland: Analysis by IS6110 restriction fragment length polymorphism and double-repetitive-element PCR

Dela, Agnieszka; Sajduda, Anna; Pawłowska, I.; Dziadek, Jarosław

doi:10.1016/j.jinf.2005.07.010

Cited by 8 publications

(6 citation statements)

References 22 publications

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“…M. tuberculosis is an infectious agent that presents both drawbacks. Among the molecular techniques used to characterize Mycobacterium strains, rRNA region polymorphism [21], the analysis of the number of IS6110 sequences [11,15,22] present in mycobacterium genome by either RFLP [22] or PCR [7,10], and DRE-PCR [7,23] are useful and practical tolls to differentiate isolates.…”

Section: Discussionmentioning

confidence: 99%

Molecular genotyping and epidemiology of Mycobacterium tuberculosis isolates obtained from inmates of correctional institutions of Campinas, Southeast Brazil

Moreira-Oliveira¹,

Oliveira²,

Pace³

et al. 2008

Braz J Infect Dis

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The objective of this study was to investigate the possible transmission of tuberculosis among 39 inmates with positive Mycobacterium tuberculosis smears in four correctional institutions located in Campinas City, SP, Brazil over a 19-month period. Fifty-one M. tuberculosis isolates from these inmates were characterized according to the number of IS6110 insertion elements present in their genomic DNA. The number of insertion elements in M. tuberculosis isolates varied from two to twelve. The dendrogram of similarity resulted in the grouping the isolates in six main clusters. These results, associated to epidemiological data, suggested the transmission of tuberculosis among inmates of the same and different institutions inmates. Univariate analysis of epidemiological data (total delay for beginning of treatment, previous treatment, and HIV status) and clustering occurrence showed that only "previous treatment" (OR = 7.65, p = 0.032) was associated with the possible transmission of tuberculosis in the studied prisons.

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Section: Discussionmentioning

confidence: 99%

Molecular genotyping and epidemiology of Mycobacterium tuberculosis isolates obtained from inmates of correctional institutions of Campinas, Southeast Brazil

Moreira-Oliveira¹,

Oliveira²,

Pace³

et al. 2008

Braz J Infect Dis

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“…PGRS-based typing, with the PGRS cloned into a recombinant plasmid, pTBN12, as a probe, was used to differentiate M. tuberculosis strains with no or low copy numbers of IS6110 (280). Further studies that employed the PGRS-RFLP method confirmed its relatively high discriminatory power (395)(396)(397). Interestingly, PGRSs were also identified in various NTM species, and consequently, a similar PGRS-RFLP assay was also used to differentiate strains of M. kansasii (398) and M. ulcerans (399).…”

Section: Methods Based On Repetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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“…The B0/W148 variant was not found in East Asian countries marked by endemicity and a high prevalence of the Beijing genotype or in a country with its own particular subset of Beijing strains (South Africa) (see Table S1 in the supplemental material). No or rare isolates of the Beijing family were recovered from permanent residents of Eastern European countries such as Poland, Bulgaria, and Romania (75,76,119,120), and it is reasonable to assume a negligible, if any, presence of the Beijing B0/ W148 variant in this part of Europe. None of the above countries received a significant influx of immigrants from the former Soviet Union, and this situation is not unexpected.…”

Section: Geographic Diversity and Place Of Origin Of B0/w148mentioning

confidence: 99%

Insights into the Origin, Emergence, and Current Spread of a Successful Russian Clone of Mycobacterium tuberculosis

Mokrousov

2013

Clin Microbiol Rev

103

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SUMMARY Mycobacterium tuberculosis variant Beijing B0/W148 is regarded as a successful clone of M. tuberculosis that is widespread in the former Soviet Union and respective immigrant communities. Understanding the pathobiology and phylogeography of this notorious strain may help to clarify its origin and evolutionary history and the driving forces behind its emergence and current dissemination. I present the first review and analysis of all available data on the subject. In spite of the common perception of the omnipresence of B0/W148 across post-Soviet countries, its geographic distribution shows a peculiar clinal gradient. Its frequency peaks in Siberian Russia and, to a lesser extent, in the European part of the former Soviet Union. In contrast, the frequency of B0/W148 is sharply decreased in the Asian part of the former Soviet Union, and it is absent in autochthonous populations elsewhere in the world. Placing the molecular, clinical, and epidemiological features in a broad historical, demographic, and ecological context, I put forward two interdependent hypotheses. First, B0/W148 likely originated in Siberia, and its primary dispersal was driven by a massive population outflow from Siberia to European Russia in the 1960s to 1980s. Second, a historically recent, phylogenetically demonstrated successful dissemination of the Beijing B0/W148 strain was triggered by the advent and wide use of modern antituberculosis (anti-TB) drugs and was due to the remarkable capacity of this strain to acquire drug resistance. In contrast, there is some indication, but not yet systematic proof, of an enhanced virulence of this strain.

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Molecular characterization of Mycobacterium tuberculosis isolates from Łódź, Poland: Analysis by IS6110 restriction fragment length polymorphism and double-repetitive-element PCR

Cited by 8 publications

References 22 publications

Molecular genotyping and epidemiology of Mycobacterium tuberculosis isolates obtained from inmates of correctional institutions of Campinas, Southeast Brazil

Molecular genotyping and epidemiology of Mycobacterium tuberculosis isolates obtained from inmates of correctional institutions of Campinas, Southeast Brazil

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Insights into the Origin, Emergence, and Current Spread of a Successful Russian Clone of Mycobacterium tuberculosis

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