Molecular Characterization of Sorghum Chlorotic Spot Virus, a Proposed Furovirus

Kendall, Timothy; Langenberg, Willem G.; Lommel, Steven A.

doi:10.1099/0022-1317-69-9-2335

Cited by 18 publications

(16 citation statements)

References 38 publications

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“…BSBMV RNAs were extracted from virions in purified preparations with chloroform and phenol (1:1) in 2× STE (34) with 1% sodium dodecyl sulfate (SDS), precipitated in three volumes of ethanol at -20°C and electrophoresed through a 1% agarose-formaldehyde denaturing gel in the presence of 0.5 µg/ml ethidium bromide (10,17,34). Nucleic acid sizes were estimated relative to a 0.24-9.5 kb RNA ladder (Gibco BRL, Gaithersburg, MD).…”

Section: Methodsmentioning

confidence: 99%

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Characteristics of Beet Soilborne Mosaic Virus, a Furo-like Virus Infecting Sugar Beet

et al. 1997

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Beet soilborne mosaic virus (BSBMV) is a rigid rod-shaped virus transmitted by Polymyxa betae. Particles were 19 nm wide and ranged from 50 to over 400 nm, but no consistent modal lengths could be determined. Nucleic acids extracted from virions were polyadenylated and typically separated into three or four discrete bands of variable size by agarose-formaldehyde gel electrophoresis. RNA 1 and 2, the largest of the RNAs, consistently averaged 6.7 and 4.6 kb, respectively. The sizes and number of smaller RNA species were variable. The molecular mass of the capsid protein of BSBMV was estimated to be 22.5 kDa. In Northern blots, probes specific to the 3′ end of individual beet necrotic yellow vein virus (BNYVV) RNAs 1–4 hybridized strongly with the corresponding BNYVV RNA species and weakly with BSBMV RNAs 1, 2, and 4. Probes specific to the 5′ end of BNYVV RNAs 1–4 hybridized with BNYVV but not with BSBMV. No cross-reaction between BNYVV and BSBMV was detected in Western blots. In greenhouse studies, root weights of BSBMV-infected plants were significantly lower than mock-inoculated controls but greater than root weights from plants infected with BNYVV. Results of serological, hybridization, and virulence experiments indicate that BSBMV is distinct from BNYVV. However, host range, capsid size, and the number, size, and polyadenylation of its RNAs indicate that BSBMV more closely resembles BNYVV than it does other members of the genus Furovirus.

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Section: Methodsmentioning

confidence: 99%

“…Members of this genus typically are composed of two to three particles of varying lengths and lack a 3ṕ oly-A tail (4, 16,17). The question of whether BNYVV should be included in the genus Furovirus because of its distinct qualities has been previously discussed (30).…”

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confidence: 99%

Characteristics of Beet Soilborne Mosaic Virus, a Furo-like Virus Infecting Sugar Beet

et al. 1997

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“…(SPMV), Wheat streak mosaic virus (WSMV) (Cabanas , 2013;Park , 2017b;Reddy , 1998;Qiu Scholthof, 2001;Seifers , 1999;Sill Agusiobo, 1955; (Bai , 2002;Kendall , 1988;Matsukura , 2015;Srinivas , 2010;Toler, 1985;Yang Mirkov, 1997), BVG RSV 2 (Park , 2017b;. …”

Section: Maize Chlorotic Mottle Virus (Mcmv) Panicum Mosaic Virus (Pmentioning

confidence: 99%

A Survey of Viral Diseases of Proso Millet (Panicum miliaceum L.) and Sorghum (Sorghum bicolor L.) in South Korea

민¹,

박²,

이³

et al. 2017

Research in Plant Disease

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Throughout year 2015 to 2016, 101 proso millet and 200 sorghum samples were collected from five provinces in South Korea. The samples were subjected to paired-end RNA sequencing and further analyzed by RT-PCR. The results indicated that Rice black-streaked dwarf virus (RBSDV) was detected from sorghum collected in Gyeongsang province. The other four viruses, including RBSDV, Rice stripe virus (RSV), Barley virus G (BVG), and Cereal yellow dwarf virus (CYDV), were detected from proso millet. Among four viruses, both RSV and RBSDV were identified high frequency from proso millet collected from Gyeongsang province. Otherwise, BVG was nearly equally identified from five provinces, suggesting that the virus was supposedly widespread nationwide. RBSDV was first identified from both proso millet and sorghum in South Korea. The other virus annotated CYDV identified proso millet was shown to have relatively low identities compared to CYDV previously reported, suggesting that the virus might be new member of Polerovirus.

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“…P. graminis is economically important because of its ability to transmit a number of different virus species, including soil borne cereal mosaic virus (SBCMV), soil borne wheat mosaic virus (SBWMV), wheat spindle streak mosaic virus (WSSMV), barley mild mosaic virus (BaMMV), barley yellow mosaic virus (BaYMV) (Kanyuka et al 2003;Kühne 2009;Tamada and Kondo 2013), peanut clump virus (PCV) (Thouvenel and Fauquet 1981), sorghum chlorotic spot virus (Kendall et al 1988) and rice stripe necrosis virus (Lozano and Morales 2009). Ward et al (1994Ward et al ( , 1998Ward et al ( and 2005 have described different P. graminis ribotypes based on sequence differences in the ribosomal rDNA region.…”

Section: Introductionmentioning

confidence: 99%

Occurrence ofPolymyxa graminisRibotypes in Germany and Their Association with Different Host Plants and Viruses

Ziegler

Fomitcheva

Zakri

et al. 2016

Cereal Research Communications

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The plasmodiophorid Polymyxa graminis transmits plant viruses to cereal crops such as wheat, rye, barley and triticale. Soil samples from different locations and cereal host plants were analyzed for the presence of P. graminis ribotypes I and II, and tested for the occurrence of soil-borne viruses. P. graminis sequences mainly from fields in Germany used for virus resistance trials, but also from a site each in Poland and Denmark were obtained and deposited in the European Nucleotide Archive. The interactions between the components of the pathogen complex -vector ribotype and virus -and the host are discussed.

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Molecular Characterization of Sorghum Chlorotic Spot Virus, a Proposed Furovirus

Cited by 18 publications

References 38 publications

Characteristics of Beet Soilborne Mosaic Virus, a Furo-like Virus Infecting Sugar Beet

Characteristics of Beet Soilborne Mosaic Virus, a Furo-like Virus Infecting Sugar Beet

A Survey of Viral Diseases of Proso Millet (Panicum miliaceum L.) and Sorghum (Sorghum bicolor L.) in South Korea

Occurrence ofPolymyxa graminisRibotypes in Germany and Their Association with Different Host Plants and Viruses

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