Molecular characterization of Sp serotype strains of infectious pancreatic necrosis virus exhibiting differences in virulence

Rb, Shivappa; Song, Haichen; Yao, Kangde; Aas-Eng, Anne; Evensen, Øystein; Vakharia, Vikram N.

doi:10.3354/dao061023

Cited by 53 publications

(118 citation statements)

References 27 publications

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“…Several studies (Bruslind & Reno 2000, Santi et al 2004, Shivappa et al 2004) have reported the presence of threonine and alanine at positions 217 and 221 of virulent strains, whereas low virulent strains have a proline and alanine at the same residues. As seen in Fig.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Molecular characterisation of Australasian isolates of aquatic birnaviruses

Davies¹,

McColl²,

Wang³

et al. 2010

Dis. Aquat. Org.

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An aquatic birnavirus, first isolated in Australia from farmed Atlantic salmon in Tasmania in 1998, has continued to be re-isolated on an infrequent but regular basis. Due to its low pathogenicity, there has been little urgency to undertake a comprehensive characterisation of this aquatic birnavirus. However, faced with possible incursions of any new aquatic birnaviruses, specific identification and differentiation of this virus from other, pathogenic, aquatic birnaviruses such as infectious pancreatic necrosis virus (IPNV) are becoming increasingly important. The present study determined the nucleic acid sequence of the aquatic birnavirus originally isolated in 1998, as well as a subsequent isolate from 2002. The sequences of the VP2 and VP5 genes were compared to that of other aquatic birnaviruses, including non-pathogenic aquatic birnavirus isolates from New Zealand and pathogenic infectious pancreatic necrosis virus isolates from North America and Europe. The deduced amino acid (aa) sequences indicate that the Australian and New Zealand isolates fall within Genogroup 5 together with IPNV strains Sp, DPL, Fr10 and N1. Thus, Genogroup 5 appears to contain aquatic birnavirus isolates from quite diverse host and geographical ranges. Using the sequence information derived from this study, a simple diagnostic test has been developed that differentiates the current Australian isolates from all other aquatic birnaviruses, including the closely related isolates from New Zealand. KEY WORDS: Aquatic birnavirus · Infectious pancreatic necrosis virus · Phylogenetic relationshipsResale or republication not permitted without written consent of the publisher OPEN PEN

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Section: Phylogenetic Analysismentioning

confidence: 99%

Molecular characterisation of Australasian isolates of aquatic birnaviruses

Davies¹,

McColl²,

Wang³

et al. 2010

Dis. Aquat. Org.

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“…Firstly, viral genomic RNA was isolated from purified virus as described previously [28]. The genomic RNA was reverse-transcribed using a random primer, and the resulting cDNAs were amplified by PCR with six specific primer pairs A1F/A1R, A2F/A2R, A3F/A3R, B1F/ B1R, B2F/B2R and B3F/B3R (Table 1) for obtaining the coding regions of segments A and B.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

“…3) [3,6,7,28,29,32]. The genomic segments A and B of POBV were analyzed as follows: 497 motifs) in addition to the two primary cleavage sites at the pVP2-VP4 and VP4-VP3 junctions (Fig.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

“…For example, infectious pancreatic necrosis virus (IPNV) is the type member of the aquatic birnaviruses and causes infectious pancreatic necrosis, an acute and serious disease in juvenile salmonid fish worldwide. Due to the ecological importance of aquatic birnaviruses, much effort has been put into studies of both biological features and the pathogenesis of aquatic birnavirus in recent years [20,[25][26][27][28]30].…”

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confidence: 99%

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Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China

Zhao

Fei

et al. 2008

Arch Virol

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A birnavirus strain, Paralichthys olivaceus birnavirus (POBV), was isolated and characterized from cultured flounder in China, and its complete genomic sequence was subsequently determined. The virus could induce cytopathic effects (CPE) in four of seven fish cell lines and was resistant to chloroform, 5-iodo-2 0 -deoxyuridine, acid and alkaline pH, and heat treatment. Purified virus particles had a typical icosahedral shape, with a diameter of approximately 55-60 nm. The genomic segments A and B of POBV were 3,091 and 2,780 bp in length and shared many of the features of the members of the family Birnaviridae. Segment A contained two partially overlapping ORFs encoding a polyprotein, pVP2-VP4-VP3, and a nonstructural protein, VP5, while segment B had only one ORF encoding for the VP1, a viral RNAdependent RNA polymerase (RdRp). This is the first report about a birnavirus strain from a new non-salmonid host in China and its complete genome sequence.

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“…Based on this sequence, TAB98 was found to cluster within Genogroup 5 along with New Zealand aquabirnaviruses that had been isolated from healthy Chinook salmon Oncorhynchus tshawtscha. Genogroup 5 consists of isolates from diverse geographical and host ranges such as pathogenic IPNV Sp116 isolated from Atlantic salmon in Norway (Shivappa et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Tasmanian aquabirnaviruses from 1998 to 2013

Mohr

Moody²,

Williams³

et al. 2015

Dis. Aquat. Org.

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Tasmanian aquabirnaviruses (TABVs) have been isolated intermittently since 1998 from healthy Atlantic salmon Salmo salar and rainbow trout Oncorhynchus mykiss farmed in Macquarie Harbour, Tasmania, Australia. However, beginning in 2011, TABVs have been isolated from rainbow trout in association with mortality events. To determine if recent molecular changes in TABV were contributing to increased mortalities, next generation sequencing was undertaken on 14 TABVs isolated from 1998 to 2013. Sequencing of both genome segments and analysis of the 5 viral proteins they encode revealed that minimal changes had occurred in the past 15 yr. Of the amino acid changes detected only 1, alanine to aspartic acid at position 139 of the minor structural VP3 protein, was unique to the recent disease events. The most dramatic changes observed were in the length of the non-structural VP5 protein varying from 43 to 133 amino acids. However, the amino acid substitution in VP3 and variable VP5 length were unlikely to have resulted in increased TABV pathogenicity. The genome of a novel Australian aquabirnavirus, Victorian trout aquabirnavirus (VTAB) was also sequenced and compared to TABV isolates.

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Molecular characterization of Sp serotype strains of infectious pancreatic necrosis virus exhibiting differences in virulence

Cited by 53 publications

References 27 publications

Molecular characterisation of Australasian isolates of aquatic birnaviruses

Molecular characterisation of Australasian isolates of aquatic birnaviruses

Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China

Molecular characterization of Tasmanian aquabirnaviruses from 1998 to 2013

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