Molecular Characterization of the <i>Fusarium graminearum</i> Species Complex in Japan

Suga, Haruhisa; Karugia, G. W.; Ward, Todd J.; Gale, Liane Rosewich; Tomimura, Kenta; Nakajima, Takashi; Miyasaka, Atsushi; Koizumi, Satoshi; Kageyama, Koji; Hyakumachi, Mitsuro

doi:10.1094/phyto-98-2-0159

Cited by 171 publications

(164 citation statements)

References 20 publications

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“…In Asia, temperature is frequently used to explain F. graminearum as the predominant species of FGSC in northern areas, while F. asiaticum dominates southern areas (Qu et al, 2008;Suga et al, 2008). However, our previous study did not corroborate this finding.…”

Section: Discussioncontrasting

confidence: 66%

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Zhang

Brankovics

Luo

et al. 2016

WMJ

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In recent years increasing demands and the relatively low-care cultivation of the crop have resulted in an enormous expansion of the acreage of maize in China. However, particularly in China, Fusarium ear rot forms an important constraint to maize production. In this study, we showed that members of both the Fusarium fujikuroi species complex (FFSC) and the Fusarium graminearum species complex are the causal agents of Fusarium ear rot in the main maize producing areas in China. Fumonisin producing Fusarium verticillioides was the most prevalent species, followed by fumonisin producing Fusarium proliferatum and 15-acetyldeoxynivalenol producing F. graminearum. Both Fusarium temperatum and Fusarium boothii were identified for the first time in the colder regions in China, extending their known habitats to colder environments. Mating type analysis of the different heterothallic FFSC species, showed that both types co-occur in each sampling site suggestive of the possibility of sexual recombination. Virulence tests with F. boothii (from maize) and F. graminearum from maize or wheat showed adaptation to the host. In addition, F. graminearum seems to outcompete F. boothii in wheat-maize rotations. Based on our findings and previous studies, we conclude that wheat/maize rotation selects for F. graminearum, while a wheat/rice rotation selects for F. asiaticum. In contrast, F. boothii is selected when maize is cultivated without rotation. A higher occurrence of F. temperatum is observed on maize in colder climatological regions in China, while Fusarium meridionale seems restricted to mountain areas. Each of these species has their characteristic mycotoxin profile and deoxynivalenol and fumonisin are the potential threats to maize production in Northern China.

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Section: Discussioncontrasting

confidence: 66%

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Zhang

Brankovics

Luo

et al. 2016

WMJ

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“…F. graminearum is also dominant in the United States and Canada (Starkey et al 2007;Gale et al 2007;Ward et al 2008;Schmale et al 2011), and is found on all continents with the exception of Antarctica (O'Donnell et al 2004;Aoki et al 2012;Backhouse 2014). While the species within the FGSC show a significant biogeographic structure (Aoki et al 2012), substantial species diversity has been observed in South America (Scoz et al 2009;Sampietro et al 2010;Umpiérrez-Failache et al 2013), Asia (Yli-Mattila et al 2009Suga et al 2008;Zhang et al 2012), Africa (Boutigny et al 2011;O'Donnell et al 2008) and New Zealand (Monds et al 2005). Long-distance dispersal and anthropogenic activities have the potential to alter regional pathogen composition as suggested by the recent discovery of significant populations of F. asiaticum, an Asian endemic that appears to be adapted to rice agroecosystems (Lee et al 2009), on wheat in major rice production areas of Louisiana and Uruguay .…”

Section: Discussionmentioning

confidence: 99%

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

et al. 2015

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As has been observed in several European countries, the frequency of Fusarium head blight (FHB) caused by members of the Fusarium graminearum species complex (FGSC) has increased in Norwegian cereals in recent years, resulting in elevated levels of deoxynivalenol in cereal grains. The objective of this study was to determine if this increase was associated with changes in FGSC composition within Norway. FGSC isolates collected from wheat, oats and barley in Norway during two periods, mainly 1993-1998 and 2004-2007, were characterized to determine species and trichothecene genotype composition and to assess levels of genetic variation and population structure. In vitro growth rates at different temperatures and aggressiveness in spring wheat were further characterized for a sub-selection of isolates. All Norwegian isolates were identified as F. graminearum. The 3-acetyl-deoxynivalenol (3-ADON) trichothecene type was dominant. However, isolates with the 15-ADON chemotype were detected in Norway for the first time and may represent a recent introduction of this trichothecene type. Bayesian-model based clustering and analyses of genetic differentiation indicated the persistence over the last 20 years of two sympatric and partially admixed populations of F. graminearum in Norway. Significant differences in average in vitro growth rates and aggressiveness were observed between these two populations. Our results demonstrate that the recent increase in prevalence of the FGSC in Norwegian cereals do not correspond to any dramatic changes in FGSC species or trichothecene chemotype composition. However, significant changes in population frequencies were observed among Norwegian F. graminearum.

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“…[10][11][12][13] Recently, genetic species identification of the F. graminearum species complex and determination of its trichothecene chemotypes by PCR-RFLP and multiplex PCR were reported. 14) In Japan, it was demonstrated that 2 species predominate among the F. graminearum species complex (Fusarium asiaticum and Fusarium graminearum s. str.).…”

Section: Introductionmentioning

confidence: 99%

“…15) Their mycotoxin-producing profiles have been examined, and the characteristics of their geographical distributions have also been reported. 14,[16][17][18][19][20][21] Metconazole ((1RS, 5RS; 1RS, 5SR)-5-(4-chlorobenzyl)-2,2-dimethyl-1-(1H-1,2,4-triazol-1-ylmethyl) cyclopentanol), a demethylation inhibitor of the ergosterol biosynthesis pathway (DMI), was synthesized and its biological activity was found by the Kureha Corporation in 1986. 22) It was first launched as a cereal fungicide in France in 1994, and had been globally registered and was being used as a fungicide for Sensitivity of Japanese Fusarium graminearum species complex isolates to metconazole cereals, oil seed rape, turf, tree nuts, fruits and many other kinds of crops in more than 30 countries by 2009.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of Japanese Fusarium graminearum species complex isolates to metconazole

et al. 2010

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The sensitivity of the Fusarium graminearum species complex, the causal fungus of Fusarium head blight, to metconazole was measured. The minimum inhibitory concentrations (MIC) of 101 isolates ranged from 0.20 to 6.25 mg/l with a single peak at 1.56 mg/l. The effective concentration for 50% growth inhibition (EC 50 ) was Ͻ0.1 mg/l in about 80% of isolates, and no isolate was significantly less sensitive to metconazole among this group. Among the F. graminearum species complex, F. asiaticum and F. graminearum s. str. were identified by PCR-RFLP. The trichothecene chemotypes were determined as 3ADON, 15ADON, or NIV by multiplex PCR. Although species-specific geographical distributions and mycotoxin production characteristics were found, the MIC values to metconazole of both species were distributed within a similar range, and no significant difference in sensitivity was observed between the species or trichothecene chemotypes. Next, CYP51 genes from isolates with different sensitivities to metconazole were amplified by PCR, and their sequences were compared. As a result, it was suggested that the differences in sensitivity to metconazole between the isolates were not due to the substitution of amino acids in CYP51, the target enzyme of DMI. Then, macrospores from isolates with various sensitivities to metconazole were sprayed onto wheat ears, and the efficacy of metconazole was examined. Metconazole showed high control activity against every isolate.

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Molecular Characterization of the Fusarium graminearum Species Complex in Japan

Cited by 171 publications

References 20 publications

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Crops are a main driver for species diversity and the toxigenic potential ofFusarium isolates in maize ears in China

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

Sensitivity of Japanese Fusarium graminearum species complex isolates to metconazole

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