Molecular characterization of two novel null waxy alleles in Mexican bread wheat landraces

Guzmán, Carlos; Ortega, Raul; Yamamori, Makoto; Peña, Roberto J.; Álvarez, José Ramón Guzmán

doi:10.1016/j.jcs.2014.11.003

Cited by 24 publications

(17 citation statements)

References 28 publications

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“…Yamamori [11] [7,9]. However, no cultivars lacking both Wx-A1 and Wx-D1 proteins, both Wx-B1 and Wx-D1 proteins, or all three Wx proteins had bee detected [11,51].…”

Section: Discussionmentioning

confidence: 99%

“…Amylopectin synthesis is more complex, involving linked reactions catalyzed by enzymes SS (SSI, SSII, and SSIII), SBE and DBE. SBE plays an important role in amylopectin synthesis, which can introduce α-1,6-glucosidic linkages into α-polyglucans [8,9]. In wild-type bread wheat, there are three GBSS I isoforms detected, one for each genome, encoded by orthologous Wx genes (Wx-A1, Wx-B1, and Wx-D1) on chromosomes 7AS, 4AL (translocated from the original 7BS) and 7DS, respectively [10,11].…”

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confidence: 99%

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Identification and molecular characterization of mutant line deficiency in three waxy proteins of common wheat (Triticum aestivum L.)

Liu¹,

Hu³

et al. 2019

Preprint

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Background: Starch is the main component of wheat (Triticum aestivum L.) grain and a key factor in determining wheat processing quality. The Wx gene encodes the granule bound starch synthase I (GBSS I) and is the sole gene responsible for amylose synthesis. Results: A waxy mutant (Wx-null) was isolated by screening M3 seeds derived from 1.0% EMS mutagenized materials with I2-KI staining of endosperm starch. SDS-PAGE electrophoresis confirmed that the Wx-null line lacked all three waxy proteins. DNA sequencing revealed three SNPs and a 3-bp InDel in the first exon, and a 16-bp InDel at the junction region of the first Wx-A1 intron from the Wx-null line. Six SNPs were identified in Wx-B1 gene of Wx-null line compared to the wild-type Gao 8901, including four missense mutations. One nonsense mutation was found at position 857 in the fourth exon, which resulted in a premature stop codon. Expression levels of Wx genes were dramatically reduced in the Wx-null line. Nonsense-mediated mRNA decay (NMD) may be triggered to degrade the non-functional Wx mRNA. There were no detectable differences in granule size and morphology between Wx-null and wild-type, but the Wx-null line contained a larger proportion of B-type starch granules. The amylose content of the Wx-null line (0.22%) was remarkably lower compared to the wild-type Gao 8901 (20.82%). Total starch is also lower in the Wx-null line. Conclusions: All three waxy proteins were non-functional in the Wx-null line. NMD may be the cause for reduced expression levels of Wx genes in the Wx-null line. The Wx-null line exhibited more B-type starch granules, dramatically lower amylose content, and decreased total starch. The Wx-null line may provide a potential waxy material with high agronomic performance in wheat breeding programs.

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“…Yamamori [11] [7,9]. However, no cultivars lacking both Wx-A1 and Wx-D1 proteins, both Wx-B1 and Wx-D1 proteins, or all three Wx proteins had bee detected [11,51].…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Identification and molecular characterization of mutant line deficiency in three waxy proteins of common wheat (Triticum aestivum L.)

Liu¹,

Hu³

et al. 2019

Preprint

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show abstract

“…The starch in wheat is present in large and small granules and comprises more than 70% of refined wheat flour. Wheat starch is composed of 22-35% amylose and 65-75% amylopectin (32). The amylose content of wheat starch is controlled by the action of waxy genes, which express granule-bound starch synthase or the waxy (Wx) proteins.…”

Section: Main Wheat Quality Traitsmentioning

confidence: 99%

CIMMYT Series on Carbohydrates, Wheat, Grains, and Health: Wheat-Based Foods: Their Global and Regional Importance in the Food Supply, Nutrition, and Health^,

et al. 2017

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“…Screening wheat genotypes for allelic variants of Waxy [1][2][3] and HMW glutenin subunits (HMW-GS) [4][5][6] is an integral part for marker-assisted selection of varieties with high value as indicators for flour-baking and technological properties of the grain. It is known that combinations of nonfunctional null alleles of Waxy genes in the genomes of wheat have a direct influence on the development of amylopectin-type starch with a reduced or complete absence of amylose, which, in turn, considerably affects the technological properties of starch and wheat flour [7][8][9].Taking into account the positive influence of HMW glutenin subunits Dx5, Dy10 (5+10), and Ax2* on the increase of flour-baking qualities, as well as the negative influence of subunits Dx2, Dy12 (2+12), and Axnull causing a reduction in baking properties of wheat, the combination of Ax2*/5+10 subunits is one of the most economically-valuable and desirable for the selection associated with high quality properties of the grain [4,5,10].…”

Section: Introductionmentioning

confidence: 99%

Screening of the genotypes of bread wheat (Triticum aestivum L.) by the allelic variants of Waxy genes and HMW glutenin subunits

et al. 2018

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Screening genotypes of wheat by allelic variants of Waxy and HMW-GS genes is a constitutional unit of marker-assisted selection of varieties with high values of desirable properties for flour-baking and technological attributes of the grain. The aim of the study was to produce a molecular screening of samples of spring soft wheat of local selection lines for the detection of genotypes of Triticum aestivum L. with valuable alleles of Waxy and HMW-GS genes. Seventy samples of wheat were subjected to DNA testing for the identification of genotypes with alleles of Waxy and HMW-GS genes. Molecular screening of wheat samples with the selected systems of molecular marking of the allelic variants of the analyzed genes made it possible to detect three partially Waxy lines with a combination of two null-alleles (Wx-A1b and Wx-B1b) in the study collection, as well as 33 plants with an economically-valuable combination of Ax2*/5+10 subunits of HMW-GS, the genotypes of which are considered as starting material for further selection work on the creation of spring soft wheat cultivars with high quality indicators of grain. The approaches applied in the study to identify Waxy and HMW-GS alleles are effective methods of evaluating their allelic polymorphism.

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Molecular characterization of two novel null waxy alleles in Mexican bread wheat landraces

Cited by 24 publications

References 28 publications

Identification and molecular characterization of mutant line deficiency in three waxy proteins of common wheat (Triticum aestivum L.)

Identification and molecular characterization of mutant line deficiency in three waxy proteins of common wheat (Triticum aestivum L.)

CIMMYT Series on Carbohydrates, Wheat, Grains, and Health: Wheat-Based Foods: Their Global and Regional Importance in the Food Supply, Nutrition, and Health^,

Screening of the genotypes of bread wheat (Triticum aestivum L.) by the allelic variants of Waxy genes and HMW glutenin subunits

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Molecular characterization of two novel null waxy alleles in Mexican bread wheat landraces

Cited by 24 publications

References 28 publications

Identification and molecular characterization of mutant line deficiency in three waxy proteins of common wheat (Triticum aestivum L.)

Identification and molecular characterization of mutant line deficiency in three waxy proteins of common wheat (Triticum aestivum L.)

CIMMYT Series on Carbohydrates, Wheat, Grains, and Health: Wheat-Based Foods: Their Global and Regional Importance in the Food Supply, Nutrition, and Health,

Screening of the genotypes of bread wheat (Triticum aestivum L.) by the allelic variants of Waxy genes and HMW glutenin subunits

Contact Info

Product

Resources

About

CIMMYT Series on Carbohydrates, Wheat, Grains, and Health: Wheat-Based Foods: Their Global and Regional Importance in the Food Supply, Nutrition, and Health^,