Molecular characterization, tissue distribution and functional analysis of macrophage migration inhibitory factor protein (MIF) in Chinese giant salamanders Andrias davidianus

Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei

“…Isopropyl-β-D-1-thiogalactopyranoside (IPTG) was added to a final concentration of 1 mM to induce protein expression at 37°C for 4 h. The bacterial cells were harvested by centrifugation at 5000 r/min for 10 min at 4°C and then disrupted by sonication in an ice bath. The His-tagged recombinant LvSDC protein in bacterial lysate was purified according to a previously described method (Wang et al, 2013). Concentration of the His-tagged LvSDC protein was tested by the Bradford method using Bradford Assay kit (Takara, China).…”

Structural Relationships Between Interstitial Cells of Cajal and Smooth Muscle Cells/Nerve Fibers in the Gastric Muscularis Mucosae of Chinese Giant Salamander

“…The Chinese giant salamander, Andrias davidianus (Amphibia: Caudata: Cryptobranchidae), is an old lineage of amphibian and a unique species in China, and is also the largest extant amphibian in the world. The amphibian is considered to have an important value in scientific research and is a key taxon to understand and reconstruct functional-evolutionary processes behind the early terricolous vertebrates (Shubin et al, 1995;Wang et al, 2013). Accordingly, A. davidianus serves as a model system for studying evolutionary and comparative biology.…”

Heterogeneous vesicles in mucous epithelial cells of posterior esophagus of Chinese giant salamander (Andrias davidianus)

“… 3 The Chinese giant salamander is regarded as having an important value in scientific research and might be a key taxon to understand and reconstruct functional-evolutionary processes behind the early land of vertebrates. 5 , 6 Hence, A. davidianus might also serve as a model system for studying cytobiological aspects of vertebrate evolution. The esophageal organization is highly variable and the feeding behavior, type of food and lifestyles might be related to esophageal specialized structure.…”