Molecular cloning and characterization of the enzyme UDP-glucose: protein transglucosylase from potato

Bocca, Silvia N.; Kissen, Ralph; Rojas-Beltran, J.; Noël, Fabrice; Gebhardt, Christiane; Moreno, Sílvia; Jardin, Patrick du; Tandecarz, Juana S.

doi:10.1016/s0981-9428(99)00117-5

Cited by 32 publications

(62 citation statements)

References 31 publications

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“…Furthermore, RGP1 does not interact with ADPGlc, a major substrate in starch synthesis in plants (Dry et al, 1992), and its biochemical properties are inconsistent with starch synthesis, as shown for the potato putative ortholog (Bocca et al, 1999). A role in cell wall biosynthesis is more likely, considering the specific association of RGP1 with the trans-Golgi compartment (Dhugga et al, 1997;Delgado et al, 1998;Bocca et al, 1999;Zhao and Liu, 2002). Zhao and Liu (2002) demonstrated that the cotton RGP gene is highly expressed during fiber development but also at the primary cell wall elongation and cell wall thickening stages, suggestive of a function in noncellulosic polysaccharide biosynthesis of the cell wall.…”

Section: Introductionmentioning

confidence: 95%

“…The protein was localized to the trans-Golgi compartment but also found in soluble extracts, leading the authors to propose that RGP1 is made on cytosolic polysomes and associates postsynthetically with Golgi membranes as a peripheral protein. Cloning of the RGP1-encoding gene from pea (Pisum sativum) and generation of specific antibodies (Dhugga et al, 1991(Dhugga et al, , 1997 led to the identification of putatively orthologous RGPs in various other plant species, such as Arabidopsis thaliana (Delgado et al, 1998), cotton (Gossypium hirsutum; Zhao and Liu, 2002), maize (Zea mays; Singh et al, 1995;Rothschild et al, 1996), potato (Solanum tuberosum; Bocca et al, 1999), wheat (Triticum aestivum), rice (Langeveld et al, 2002), and tomato (Solanum lycopersicum; Selth et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Early studies implicated RGPs of different species such as maize (Singh et al, 1995;Rothschild et al, 1996), potato (Bocca et al, 1999), and wheat (Langeveld et al, 2002) in starch synthesis, most likely due to their occurrence in starch-synthesizing tissues such as endosperm or potato tubers. However, this hypothesis was proven implausible, as RGPs do not contain a transit peptide for targeting into plastids.…”

Section: Introductionmentioning

confidence: 99%

“…However, this hypothesis was proven implausible, as RGPs do not contain a transit peptide for targeting into plastids. Furthermore, RGP1 does not interact with ADPGlc, a major substrate in starch synthesis in plants (Dry et al, 1992), and its biochemical properties are inconsistent with starch synthesis, as shown for the potato putative ortholog (Bocca et al, 1999). A role in cell wall biosynthesis is more likely, considering the specific association of RGP1 with the trans-Golgi compartment (Dhugga et al, 1997;Delgado et al, 1998;Bocca et al, 1999;Zhao and Liu, 2002).…”

Section: Introductionmentioning

confidence: 99%

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The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development inArabidopsis

et al. 2011

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L-Ara, an important constituent of plant cell walls, is found predominantly in the furanose rather than in the thermodynamically more stable pyranose form. Nucleotide sugar mutases have been demonstrated to interconvert UDP-Larabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf) in rice (Oryza sativa). These enzymes belong to a small gene family encoding the previously named Reversibly Glycosylated Proteins (RGPs). RGPs are plant-specific cytosolic proteins that tend to associate with the endomembrane system. In Arabidopsis thaliana, the RGP protein family consists of five closely related members. We characterized all five RGPs regarding their expression pattern and subcellular localizations in transgenic Arabidopsis plants. Enzymatic activity assays of recombinant proteins expressed in Escherichia coli identified three of the Arabidopsis RGP protein family members as UDP-L-Ara mutases that catalyze the formation of UDP-Araf from UDP-Arap. Coimmunoprecipitation and subsequent liquid chromatography-electrospray ionization-tandem mass spectrometry analysis revealed a distinct interaction network between RGPs in different Arabidopsis organs. Examination of cell wall polysaccharide preparations from RGP1 and RGP2 knockout mutants showed a significant reduction in total L-Ara content (12-31%) compared with wild-type plants. Concomitant downregulation of RGP1 and RGP2 expression results in plants almost completely deficient in cell wall-derived L-Ara and exhibiting severe developmental defects.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development inArabidopsis

et al. 2011

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“…The RGPs of a number of species have been studied: Arabidopsis (Arabidopsis thaliana; Delgado et al, 1998), maize (Zea mays; Rothschild and Tandecarz, 1994;Sagi et al, 2005), potato (Solanum tuberosum; Bocca et al, 1999), wheat (Triticum aestivum) and rice (Orzya sativa; Langeveld et al, 2002), cotton (Gossypium hirsutum; Zhao et al, 2001), and tomato (Lycopersicon esculentum; Selth et al, 2006). The RGPs of wheat, rice, and potato have been implicated in the initiation of starch biosynthesis (Bocca et al, 1997;Langeveld et al, 2002), although there is no direct evidence that confirms this hypothesis.…”

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confidence: 99%

Arabidopsis Reversibly Glycosylated Polypeptides 1 and 2 Are Essential for Pollen Development

et al. 2006

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Reversibly glycosylated polypeptides (RGPs) have been implicated in polysaccharide biosynthesis. To date, to our knowledge, no direct evidence exists for the involvement of RGPs in a particular biochemical process. The Arabidopsis (Arabidopsis thaliana) genome contains five RGP genes out of which RGP1 and RGP2 share the highest sequence identity. We characterized the native expression pattern of Arabidopsis RGP1 and RGP2 and used reverse genetics to investigate their respective functions. Although both genes are ubiquitously expressed, the highest levels are observed in actively growing tissues and in mature pollen, in particular. RGPs showed cytoplasmic and transient association with Golgi. In addition, both proteins colocalized in the same compartments and coimmunoprecipitated from plant cell extracts. Single-gene disruptions did not show any obvious morphological defects under greenhouse conditions, whereas the double-insertion mutant could not be recovered. We present evidence that the double mutant is lethal and demonstrate the critical role of RGPs, particularly in pollen development. Detailed analysis demonstrated that mutant pollen development is associated with abnormally enlarged vacuoles and a poorly defined inner cell wall layer, which consequently results in disintegration of the pollen structure during pollen mitosis I. Taken together, our results indicate that RGP1 and RGP2 are required during microspore development and pollen mitosis, either affecting cell division and/or vacuolar integrity.

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Proteomic response to iron deficiency in tomato root

Li¹,

Wu²,

Hao³

et al. 2008

Proteomics

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To know the root adjustment in response to iron deficiency, differentially displayed proteins in tomato roots of wild type and its iron uptake inefficient mutant T3238fer were analyzed by 2-DE and MALDI-TOF MS-based proteomic method under iron sufficiency and deficiency. Ninety-seven proteins were identified, 63 of them were classified in various metabolic pathways. About 40 proteins involved in starch degradation, TCA and ascorbate cycles were upregulated under iron deficiency and grouped in a network together with glycolysis, whereas proteins for fructose metabolism were decreased. Proteins involved in methionine synthesis, cell wall synthesis, mitochondria ATP synthesis, vacuole ATPase, HSP70/90, etc. also revealed enhanced expression under iron deficiency, while proteins about redox homeostasis, transcription factors, kinases, etc. showed diversified changes. The responses are closely associated with energy metabolism, organic acid formation, root morphological change, redox and sulfur homeostasis, and signal transduction, which enhance iron uptake, reutilization and other adaptive changes. Most of the proteins affected by iron deficiency and fer mutation showed similar effect on individual proteins or pathways, but the independent function of FER to iron deficiency were statistically indicated.

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Molecular cloning and characterization of the enzyme UDP-glucose: protein transglucosylase from potato

Cited by 32 publications

References 31 publications

The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development inArabidopsis

The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development inArabidopsis

Arabidopsis Reversibly Glycosylated Polypeptides 1 and 2 Are Essential for Pollen Development

Proteomic response to iron deficiency in tomato root

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