Molecular cloning and characterization of the sheep α-TTP gene and its expression in response to different vitamin E status

Liu, Kun; Luo, Haiwei; Yue, Du-bing; Ge, Su-yun; Yuan, Fei; Yan, Le-yan; Jia, Huina

doi:10.1016/j.gene.2011.12.006

Cited by 11 publications

(8 citation statements)

References 37 publications

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“…Lambs were assigned on the basis of body weight (BW) to one of five dietary treatments being a control diet without added vit E (CG) and four additional diets (VG) containing 20, 100, 200, and 2000 IU d -1 lamb -1 of vit E (α-tocopheryl acetate), respectively. These dose levels were 0, 1, 5, 10, and 100 times that of the NRC (1985) feeding standard, which was chosen according to our previous studies Yan et al, 2010;Ge et al, 2011;Liu et al, 2012).…”

Section: Experiments Design and Dietary Treatmentsmentioning

confidence: 99%

Effect of vitamin E supplementation on growth performance, carcass characteristics and intramuscular fatty acid composition of Longissimus dorsi muscle in Tan sheep

Zhao

Luo

Zhang

et al. 2013

Chilean J. Agric. Res.

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The beneficial effects on meat quality of adding vitamin E to diets have been extensively studied in cattle and lamb. However, how supplemental vit E affects the performance and meat quality of ʽTanʼ sheep has not been reported. Thirtyfive male lambs of ʽTanʼ sheep (20-30 d after weaning) with similar body weight were randomly divided into five groups of seven each to receive a basal diet plus five levels of vit E supplementation: 0 (control group, CG), 20, 100, 200, and 2000 IU d -1 lamb -1 . The results showed: 1) no differences were observed in animal growth performance between CG and vit E treated groups (VG) (P > 0.05); 2) vit E supplementation levels over 200 IU d -1 lamb -1 dramatically suppressed subcutaneous fat deposition and similarly that above 100 IU d -1 lamb -1 considerably reduced drip loss and pH 24 h of Longissimus dorsi (LD) muscle in VG lambs compared to those of CG (P < 0.01), but with no significant effect on other carcass characteristics (P > 0.05); 3) although vit E supplementation generally did not affect the fatty acid composition of intramuscular lipids in LD muscle, the percentage of n-6 polyunsaturated fatty acids (PUFA) and the ratio of n-6/n-3 PUFA were significantly enhanced (P < 0.05) and an increasing tendency (P < 0.1) for both total PUFA proportion and the ratio of PUFA/saturated fatty acids (SFA) was also observed in ʽTanʼ sheep lambs treated with vit E. In conclusion, vit E supplementation of male ʽTanʼ sheep lambs did not influence growth performance, significantly reduced subcutaneous fat deposition, drip loss, pH 24 h and tended to improve fatty acid profile of LD muscle.

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Section: Experiments Design and Dietary Treatmentsmentioning

confidence: 99%

Effect of vitamin E supplementation on growth performance, carcass characteristics and intramuscular fatty acid composition of Longissimus dorsi muscle in Tan sheep

Zhao

Luo

Zhang

et al. 2013

Chilean J. Agric. Res.

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“…We succeeded in amplifying and sequencing a full-length α-TTP cDNA from bovine liver (GenBank accession: LC223005). The cDNA contains 2740 nucleotides and the open reading frame contains 846 bp encoding 282 amino acids with 88%,83%,83% and 98% identity with the human [6], rat [9], mouse [14] and sheep [15] orthologs, respectively (Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

“…Bovine α-TTP has an additional 5 amino acids (GEEVT) at the C terminus, which rat, human, mouse α-TTPs do not have. Sheep α-TTP has similar 5 amino acid C-terminal sequence (GEEVI) [15]. A protein BLAST search revealed that many Cetartiodactyla animals, such as water buffalo, bison, goat, deer, antelope, yak, camel, dolphin, whale, and killer whale, have similar Cterminal sequences, while other mammals (primates, rodents, bat, leopard, mole and Perissodactyla animals, such as horse, donkey and rhinoceroses) do not have them.…”

Section: Resultsmentioning

confidence: 99%

Molecular Cloning and Expression Analysis of Bovine Alpha-tocopherol Transfer Protein (α-TTP)

Taguchi¹,

Komatsu-Tanaka²,

Hirose³

et al. 2017

ARRB

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Aims: To understand the mechanisms by which cattle circulate and accumulate vitamin E, we cloned the cDNA for bovine α-tocopherol transfer protein (α-TTP) and examined its expression in different tissues. Methodology: A full length α-TTP cDNA was amplified from bovine liver by RT-PCR. Poly (A) + RNA obtained from bovine tissues was subjected to RT-PCR analysis to examine α-TTP mRNA expression. Western blot analysis was performed using polyclonal antibody raised against an oligopeptide derived from bovine α-TTP to examine α-TTP protein expression in various bovine tissues. The localization of α-TTP in bovine lung tissue was examined by immunostaining with antibovine α-TTP polyclonal antibody.

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“…These rings are moderately polar, giving them an affinity for the cellular membrane surface while the phytyl tail is hydrophobic and normally associated with membrane lipids [18]. These structural features of RRR -α-tocopherol are efficiently acted on by the human hepatic α-TTP which is responsible for maintaining plasma α-tocopherol concentrations [19]. …”

Section: Resultsmentioning

confidence: 99%

Biomass and RRR-α-tocopherol production in Stichococcus bacillaris strain siva2011 in a balloon bioreactor

2014

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BackgroundGreen microalgae represent a renewable natural source of vitamin E. Its most bioactive form is the naturally occurring RRR-α-tocopherol which is biosynthesized in photosynthetic organisms as a single stereoisomer. It is noteworthy that the natural and synthetic α-tocopherols are different biomolecular entities. This article focuses on RRR-α-tocopherol production in Stichococcus bacillaris strain siva2011 biomass in a bioreactor culture with methyl jasmonate (MeJa) elicitor. Additionally, a nonlinear mathematical model was used to quantitatively scale-up and predict the biomass production in a 20 L balloon bioreactor with dual variables such as time and volume.ResultsApproximately 0.6 mg/g dry weight (DW) of RRR-α-tocopherol was enhanced in S. bacillaris strain siva2011 biomass with the MeJa 50 μL/L for 24 hrs elicitations when compared to the control. The R2 value from the nonlinear model was enhanced up to 95% when compared to the linear model which significantly improved the accuracy for estimating S. bacillaris strain siva2011 biomass production in a balloon bioreactor.ConclusionsS. bacillaris strain siva2011 is a new green microalga which biosynthesizes significant amounts of RRR-α-tocopherol. Systematically validated dual variable empirical data should provide key insights to multivariable or fourth order modeling for algal biomass scale-up. This bioprocess engineering should provide valuable information for industrial production of RRR-α-tocopherol from green cells.

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Molecular cloning and characterization of the sheep α-TTP gene and its expression in response to different vitamin E status

Cited by 11 publications

References 37 publications

Effect of vitamin E supplementation on growth performance, carcass characteristics and intramuscular fatty acid composition of Longissimus dorsi muscle in Tan sheep

Effect of vitamin E supplementation on growth performance, carcass characteristics and intramuscular fatty acid composition of Longissimus dorsi muscle in Tan sheep

Molecular Cloning and Expression Analysis of Bovine Alpha-tocopherol Transfer Protein (α-TTP)

Biomass and RRR-α-tocopherol production in Stichococcus bacillaris strain siva2011 in a balloon bioreactor

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