Molecular Cloning and Characterization of a Mouse Gene with Homology to the Duffy-Antigen Receptor for Chemokines

Tang, Tong Boon; Owen, James D.; Du, Jianguo; Walker, Cheryl L.; Richmond, Ann

doi:10.3109/10425179809072188

Cited by 11 publications

(5 citation statements)

References 39 publications

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“…We suspect that regulator DARC may also participate angiogenesis in breast cancer. Although some studies had shown that DARC is expressed in several kinds of tumors, including erythroleukemia Inhibition of breast cancer growth and metastasis by DARC J Wang et al , glioblastoma (Desbaillets et al, 1997) and hemangiosarcomas (Tang et al, 1998), the function of DARC in tumors remains unknown. In order to investigate the effects and the mechanisms of DARC in breast tumor, we established DARC overexpressing MDA-MB-435HM and MDA-MB-231 cell lines by stable transfection.…”

Section: Discussionmentioning

confidence: 99%

Enhanced expression of Duffy antigen receptor for chemokines by breast cancer cells attenuates growth and metastasis potential

et al. 2006

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In addition to the role in regulating leukocyte trafficking, chemokines recently have been shown to be involved in cancer growth and metastasis. Chemokine network in tumor neovascularity may be regulated by decoy receptors. Duffy antigen receptor for chemokines (DARC) is a specific decoy receptor binding with the angiogenic CC and CXC chemokines. To investigate the effects of DARC on the tumorigenesis and the metastasis potential of human breast cancer cells, human DARC cDNA was reintroduced into the MDA-MB-231 and MDA-MB-435HM cells which have a high capability of spontaneous pulmonary metastasis. We demonstrated that DARC overexpression induced inhibition of tumorigenesis and/ or metastasis through interfering with the tumor angiogenesis in vivo. This inhibition is associated with decreasing CCL2 protein levels, and MVD and MMP-9 expression in xenograft tumors. In human breast cancer samples, we also demonstrated that low expression of the DARC protein is significantly associated with estrogen receptor (ER) status, MVD, lymph node metastasis, distant metastasis and poor survival. Our results suggest for the first time that DARC is a negative regulator of growth in breast cancer, mainly by sequestration of angiogenic chemokines and subsequent inhibition of tumor neovascularity.

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Section: Discussionmentioning

confidence: 99%

Enhanced expression of Duffy antigen receptor for chemokines by breast cancer cells attenuates growth and metastasis potential

et al. 2006

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“…XCR1 is the receptor for XCL1 (lymphotactin) and CX3CR1 for CX3CL1 (fractalkine) (see ref [19] for review). A chemokine-binding protein, also known as the Duffy antigen receptor for chemokines, has been shown to bind promiscuously to CXC and CC chemokines [20]. In addition, other 7TM GPCR, encoded by herpes-and poxviruses, have been identified [21,22].…”

Section: Chemokines and Their Receptorsmentioning

confidence: 99%

Role of CXCL1 in tumorigenesis of melanoma

Dhawan

Richmond

2002

Journal of Leukocyte Biology

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258

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The CXC chemokine, CXCL1 (melanoma growth-stimulatory activity/growth-regulated protein α), plays a major role in inflammation, angiogenesis, tumorigenesis, and wound healing. Recently, chemokines have been extensively related to cellular transformation, tumor growth, homing, and metastasis. CXCL1 and its mouse homologue MIP-2 have been shown to be involved in the process of tumor formation. When chemokines such as CXCL1 and CXCL8 (IL-8) become disregulated so that they are chronically expressed, tissue damage, angiogenesis, and tumorigenesis can follow. This up-regulation of chemokines has been attributed to constitutive activation of NF-κB. The constitutive NF-κB activation is an emerging hallmark in various types of tumors including breast, colon, pancreatic, ovarian, as well as melanoma. Previous findings from our laboratory and other laboratories have demonstrated the role of endogenous activation of NF-κB in association with enhanced metastatic potential of malignant melanoma cells and suggest that targeting NF-κB may have potential therapeutic effects in clinical trials. An important step in this direction would be to delineate the important intracellular pathways and upstream kinases involved in up-regulation of NF-κB in melanoma cells. In this review, the signaling pathways involved in the disregulation of NF-κB and chemokine expression are discussed.

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“…It was reported that DARC was expressed in some tumors including erythroleukemia, glioblastoma, and hemangiosarcomas [8][9][10], and that the microvessel density (MVD) was commonly considered a consistent indicator of angiogenesis in human cancer [11]. In tissue sections, angiogenesis could be highlighted immunohistochemically using antibodies against vascular endothelium such as CD34, factor VIIIrelated antigen, or CD31 [12].…”

Section: Introductionmentioning

confidence: 99%

Duffy antigen receptor for chemokines in laryngeal squamous cell carcinoma as a negative regulator

Sun

Wang

Zhu

et al. 2010

Acta Oto-Laryngologica

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It was found that DARC was expressed in the LSCC samples, with a high expression in 32 of our cases (49.2%), and in lymph node-negative and -positive groups of 24 (72.7%) and 8 cases (25%), respectively (p < 0.01). MVD was (24.75 ± 5.45)/High power field (HP) and (32.58 ± 5.23)/HP in the cases with high and low expression of DARC, respectively (p < 0.01). More importantly, the DARC expression presented a strong negative correlation with nodal metastasis, with pTNM stage, and with recurrence, and the cases with a high DARC expression indicated a higher survival rate. In addition, the multivariate analysis showed that the DARC expression was an independent prognostic factor (p = 0.032; hazard ratio, 21.476; 95% confidence interval, 1.297-355.68).

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Molecular Cloning and Characterization of a Mouse Gene with Homology to the Duffy-Antigen Receptor for Chemokines

Cited by 11 publications

References 39 publications

Enhanced expression of Duffy antigen receptor for chemokines by breast cancer cells attenuates growth and metastasis potential

Enhanced expression of Duffy antigen receptor for chemokines by breast cancer cells attenuates growth and metastasis potential

Role of CXCL1 in tumorigenesis of melanoma

Duffy antigen receptor for chemokines in laryngeal squamous cell carcinoma as a negative regulator

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