Molecular cloning and characterization of GbMECT and GbMECP gene promoters from Ginkgo biloba

Cheng, Shuiyuan; Li, L L; Yuan, Honghui; Xu, Feng; Cheng, Hua

doi:10.4238/2015.november.24.20

Cited by 10 publications

(4 citation statements)

References 56 publications

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“…Previous studies have proved that by up-regulating expression levels of 4 key genes (GbDXS, GbDXR, GbGGPPS, GbLPS and GbMECPs) in TTL synthesis pathway, CCC could promote biosynthesis of TTL in G. biloba (Xu et al, 2011;Cheng et al, 2015), and increase contents of ginkgolide and bilobalide (Xu et al, 2011). CCC treatment could significantly increase transcription levels of ERF-25 and ERF-27, implying that they could participate in the regulation of CCC on Ginkgo synthesis in Ginkgo.…”

Section: Uv-b Drought and CCC Treatments Regulate The Transcription Of Ap2-erfsmentioning

confidence: 99%

“…ERF13 and ERF27 mainly regulated bDXS, GbDXR and MECPs. UV treatment could significantly increase transcription levels of MECPs gene and total lactone content (Pingsheng and Shuchai, 2002;Cheng et al, 2015). Moreover, drought could significantly increase the accumulation of TTL in Ginkgo (Zhu et al, 2011).…”

Section: Uv-b Drought and CCC Treatments Regulate The Transcription Of Ap2-erfsmentioning

confidence: 99%

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Transcriptome-based Discovery of AP2/ERF Transcription Factors Related to Terpene Trilactones Synthesis in Ginkgo biloba

Yuan

Zha

et al. 2019

Not Bot Horti Agrobo

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Ginkgo biloba is a unique tree in China with medicinally and phylogenetically important characteristics. Terpene trilactones (TTL) is a key active pharmaceutical ingredient in Ginkgo, so the content of TTL in Ginkgo has become one of the important indices for evaluating quality of the medicinal materials. By transcriptome sequencing on samples treated by chlormequat, ultraviolet (UV) and drought, totally 59820 contigs and 37564 unigenes were obtained. Furthermore, 18234 unigenes were annotated through COG, KEGG and GO analysis. There were 78 AP2/ERF transcription factors, 23 factors of up-regulation and 66 factors of down-regulation that were related with synthetic pathway of TTL in Ginkgo. Phylogenetic tree clustering analysis indicated that there were 42 AP2s could be clustered into ERF, DREB and RVA subfamilies. EMSA analysis demonstrated that GbERF13, GbERF25 and GbERF27 could bind with regulatory elements, such as E-box, in the upstream of GbMECPs promoter. Expression analysis showed that the expression level of GbERF25 was the highest in root, and GbERF25 and GbERF27 were expressed in relatively high transcription levels in leaf and other tissues. The results of qRT-PCR indicated that CCC treatment could significantly improve expression levels of ERF25 and ERF27, and UV and drought could induce transcription levels of ERF13 and ERF25, respectively. The results implied that ERF25 and ERF27 might involve in the induction and regulation of CCC treatment on synthesis of bilobalide in G. biloba. ERF13 might participate in the regulation of bilobalide synthesis induced by UV, and EFR25 might involve in the regulation of the synthesis induced by drought. During annual cycle of expression, the transcription levels of ERF13, ERF25 and ERF27 had significantly positive correlation with diterpene level with correlation coefficient 0.975. It implied that these transcription factors mainly acted on the MEP pathway that regulated synthesis of bilobalide. The aim of the research was to indicate the mechanism of environment or cultivation measure regulating target gene of TTL metabolic pathway by AP2/ERF, and establish metabolic network of AP2/ERF regulating TTL synthesis. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Section: Uv-b Drought and CCC Treatments Regulate The Transcription Of Ap2-erfsmentioning

confidence: 99%

Section: Uv-b Drought and CCC Treatments Regulate The Transcription Of Ap2-erfsmentioning

confidence: 99%

Transcriptome-based Discovery of AP2/ERF Transcription Factors Related to Terpene Trilactones Synthesis in Ginkgo biloba

Yuan

Zha

et al. 2019

Not Bot Horti Agrobo

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“…OsWRKY74 modulates tolerance to phosphate starvation in rice (Dai et al, 2016). The WRKY promoter regions of several ginkgo terpene trilactone biosynthetic gene, such as GbLPS (Kim et al, 2012), GbDXS (Xu et al, 2013), GbGGPPS (Xu et al, 2013), GbHMGR (Liao et al, 2015a), GbMVD (Liao et al, 2015b), GbIDS (Kang et al, 2013), GbMECP and GbMECT (Cheng et al, 2015). However, knowledge on the function of ginkgo WRKY genes is limited because on the limited works that was previously reported (Liao et al, 2015c;Liao et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of WRKY Family Genes and Analysis of Their Expression in Response to Abiotic Stress in Ginkgo biloba L.

Cheng

Liu

Liao

et al. 2019

Not Bot Horti Agrobo

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Ginkgo biloba is widely planted, and the extracts of leaves contain flavonoids, terpene esters and other medicinal active ingredients. WRKY proteins are a large transcription factor family in plants, which play an important role in the regulation of plant secondary metabolism and development, as well as the response to biotic and abiotic stress. In our study, we identified 40 genes with conserved WRKY motifs in the G. biloba genome and classified into groups I (groups I-N and -C), II (groups IIa, b, c, d, and e), and III, which include 12, 26, and 2 GbWRKY genes, respectively. Meanwhile, the expression patterns of 10 GbWRKY (GbWRKY2, GbWRKY3, GbWRKY5, GbWRKY7, GbWRKY11, GbWRKY15, GbWRKY23, GbWRKY29, GbWRKY31, GbWRKY32) under different tissue and abiotic stress conditions were analyzed. Under stress treatment, the expression patterns of 10 WRKY genes were changed. 10 ginkgo WRKY transcription factors were induced by ETH and SA, but there are two different induced response modes. The expression of 10 WRKY genes was inhibited under low temperature, high temperature and MeJA hormone induction. Most WRKY genes were up-regulated under the induction of high salt and ABA. GbWRKYs were differentially expressed in various tissues after abiotic stress and plant hormone treatments, thereby indicating their possible roles in biological processes and abiotic stress tolerance and adaptation. Our results provided insight into the genome-wide identification of GbWRKYs, as well as their differential responses to stresses and hormones. These data can also be utilized to identify potential molecular targets to confer tolerance to various stresses in G. biloba. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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“…Further, some studies suggested that POD activity of litchi fruit stored at room temperature was higher than the wet storage. POD have multi functional gene in Ginkgo biloba as it has the potential function on defense aspects, such as it participates in the removing of heavy metal pollution and dealing with the damage (Cheng et al,2010).…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression pattern analysis of MmPOD12 gene in mulberry under abiotic stresses

Li¹,

Chen²,

Kotoka³

et al. 2017

JEBAS

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A full-length cDNA denominated as MmPOD12 for peroxidase in mulberry(Morus alba), an enzyme involved in the respiration, photosynthesis, and the oxidation of auxin, was cloned from 'Yu71-1' a variety of mulberry using a rapid amplification of terminal (RACE) approach. The full cDNA of MmPOD12 has1482 base pairs (bp) in length with an open reading frame (ORF) 1050 bp encoding a protein of 349 amino acids residues with a predicted molecular weight of 53.92 kDa and an isoelectric point of 9.35. Sequence analysis revealed that MmPOD12 shares homology with the Morus notabili (M. notabilis C.K.Schn) and has closely related to green plum, strawberry and pear. The expression patterns of MmPOD12 treated with drought, salt and hormones stresses were examined using real-time quantitative PCR (RT-qPCR). These experiments caused significant up-regulation of the expression of MmPOD12 under drought and salt stress. The highest expression level of MmPOD12 appeared at 2d for salt stress, and 7d for drought stress, while a significant fluctuation of MmPOD12 expression was detected after ABA and SA stresses. These findings provide a basis for future functional analyses of MmPOD12 gene in Mulberry.

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Molecular cloning and characterization of GbMECT and GbMECP gene promoters from Ginkgo biloba

Cited by 10 publications

References 56 publications

Transcriptome-based Discovery of AP2/ERF Transcription Factors Related to Terpene Trilactones Synthesis in Ginkgo biloba

Transcriptome-based Discovery of AP2/ERF Transcription Factors Related to Terpene Trilactones Synthesis in Ginkgo biloba

Genome-Wide Identification of WRKY Family Genes and Analysis of Their Expression in Response to Abiotic Stress in Ginkgo biloba L.

Cloning and expression pattern analysis of MmPOD12 gene in mulberry under abiotic stresses

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