Molecular cloning and characterization ofp38gene in the Chinese Mitten Crab,Eriocheir sinensis

Abstract

p38 is an important component of the MAPK signaling pathways as a Ser/Thr protein kinase. We cloned the full length p38 (Es-p38) cDNA sequence in Eriocheir Sinensis which contained a 1098nucleotide ORF that encoded a protein of 365 amino acids. Es-p38 protein has a p38 MAPK functional domain (STKc_p38), it is a typical serine/threonine kinase and processes all p38 protein's conservative T-G-Y motif. Tissue expression of the Es-p38 gene and expression during testis development stages were determined by quantitative real-time PCR analysis. The results show that Es-p38 gene is expressed in various tissues, but it expresses higher in the muscle, hepatopancreas, intestine, ovary and testis. The expression level of Es-p38 gene at different stages of testis development shows no significant difference between the spermatocyte phase (July-August) and spermatid stage (August-October), but it rises significantly in the mid-late period of sperm stage (December-January). This changing trend has a positive relationship with the E. Sinensis gonad development and reproduction stage, which preliminarily shows that Es-p38 may function in the E. Sinensis testis development and the process of spermatogenesis.

Molecular Cloning and Expression Determination of p38 MAPK from the Liver and Kidney of Silver Carp

“… found that p38 is highly expressed in the muscle of Branchiostoma belcheri . Similarly, in Eriocheir sinensis and Litopenaeus vannamei , muscle is also the tissue with highest expression rate of p38 . Our results showed that Hm‐p38a expresses in all examined tissues, but muscle shows the highest relative expression rate.…”

Identification and characterization of two novel peritrophic membrane (PM) genes in the Chinese mitten crab Eriocheir sinensis that exhibit activity against high-pH stress and Aeromonas hydrophila challenge

“…Diluted synthesized cDNA was used as a template. This analysis was conducted on the CFX96™ Real‐Time System (Bio‐Rad), using two pair gene‐specific primers (Per1‐Q‐F, Per1‐Q‐R, Per2‐Q‐F, Per2‐Q‐R; Table ), with β‐actin (GenBank accession number: KY356885.1) as an internal control gene (Zhao, Guo, et al, ; Zhao, Wang, et al, ). Studies have shown that β‐actin is one of the most reliable reference genes and had been used widely in E. sinensis after its stability being verified (Fang et al, ; Huang et al, ; Li et al, ; Liu, Wang, et al, ; Mu et al, ; Xu et al, ).…”

“…Table 1), with β-actin (GenBank accession number: KY356885.1) as an internal control gene (Zhao, Guo, et al, 2016;Zhao, Wang, et al, 2016). Studies have shown that β-actin is one of the most reliable reference genes and had been used widely in E. sinensis after its stability being verified (Fang et al, 2016;Huang et al, 2015;Li et al, 2008;Mu et al, 2009;Xu et al, 2016).…”

P38 participates in spermatogenesis and acrosome reaction prior to fertilization in Chinese mitten crab Eriocheir sinensis

“…In a separate study, we were able to successfully clone the full-length p38 cDNA sequence of the Chinese mitten crab Eriocheir sinensis (GenBank accession no. KF582665) and confirmed it to be a homologue of p38α (MAPK14) by sequence alignment (Zhao et al, 2014).…”