Molecular Cloning and Characterization of a Novel Jasmonate Inducible Pathogenesis-Related Class 10 Protein Gene, JIOsPR10, from Rice (Oryza sativa L.) Seedling Leaves

Jwa, Nam‐Soo; Agrawal, Ganesh Kumar; Rakwal, Randeep; Park, Chan Ho; Agrawal, Vishwanath Prasad

doi:10.1006/bbrc.2001.5507

Cited by 141 publications

(88 citation statements)

References 43 publications

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“…Consistent with previous reports (Ahn et al, 2005), treatment with 0.1 mM JA yielded no significant protection against C. miyabeanus (Fig. 1A), even though this concentration is high enough to induce JA-responsive JIOsPR10 transcription (Jwa et al, 2001). Higher concentrations of JA also failed to trigger induced resistance (data not shown), suggesting that JA is not a major signal for the activation of defenses against C. miyabeanus.…”

Section: Exogenous Aba Treatment Induces Resistance Against C Miyabesupporting

confidence: 92%

“…5B). Similar results were obtained when monitoring the expression of the JA-inducible defense gene JIOsPR10 (Jwa et al, 2001) in leaves of CO39 plants upon infection with Cm988. As expected, JIOsPR10 mRNAs accumulated to high levels in inoculated control plants, resulting in an approximately 9-fold induction relative to mock-treated plants by 48 hpi (Fig.…”

Section: Influence Of Exogenous Aba Treatment On Pathogenesis-relatedsupporting

confidence: 79%

“…5B; data not shown). Moreover, in accordance with the failure of exogenous JA to induce brown spot resistance, transcriptional analysis of the JA-inducible JIOsPR10 gene (Jwa et al, 2001) did not reveal any primed activity of the JA defense pathway in ABAtreated plants (Figs. 1 and 5B).…”

Section: Discussionsupporting

confidence: 58%

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Abscisic Acid-Induced Resistance against the Brown Spot Pathogen Cochliobolus miyabeanus in Rice Involves MAP Kinase-Mediated Repression of Ethylene Signaling

et al. 2010

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The plant hormone abscisic acid (ABA) is involved in an array of plant processes, including the regulation of gene expression during adaptive responses to various environmental cues. Apart from its well-established role in abiotic stress adaptation, emerging evidence indicates that ABA is also prominently involved in the regulation and integration of pathogen defense responses. Here, we demonstrate that exogenously administered ABA enhances basal resistance of rice (Oryza sativa) against the brown spot-causing ascomycete Cochliobolus miyabeanus. Microscopic analysis of early infection events in control and ABAtreated plants revealed that this ABA-inducible resistance (ABA-IR) is based on restriction of fungal progression in the mesophyll. We also show that ABA-IR does not rely on boosted expression of salicylic acid-, jasmonic acid -, or callosedependent resistance mechanisms but, instead, requires a functional Ga-protein. In addition, several lines of evidence are presented suggesting that ABA steers its positive effect on brown spot resistance through antagonistic cross talk with the ethylene (ET) response pathway. Exogenous ethephon application enhances susceptibility, whereas genetic disruption of ET signaling renders plants less vulnerable to C. miyabeanus attack, thereby inducing a level of resistance similar to that observed on ABA-treated wild-type plants. Moreover, ABA treatment alleviates C. miyabeanus-induced activation of the ET reporter gene EBP89, while derepression of pathogen-triggered EBP89 transcription via RNA interference-mediated knockdown of OsMPK5, an ABA-primed mitogen-activated protein kinase gene, compromises ABA-IR. Collectively, these data favor a model whereby exogenous ABA enhances resistance against C. miyabeanus at least in part by suppressing pathogen-induced ET action in an OsMPK5-dependent manner.

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Section: Exogenous Aba Treatment Induces Resistance Against C Miyabesupporting

confidence: 92%

Section: Influence Of Exogenous Aba Treatment On Pathogenesis-relatedsupporting

confidence: 79%

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Abscisic Acid-Induced Resistance against the Brown Spot Pathogen Cochliobolus miyabeanus in Rice Involves MAP Kinase-Mediated Repression of Ethylene Signaling

et al. 2010

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“…The induced expression of PR10 genes in response to attacks by fungus pathogens has been widely investigated. For example, the blast pathogen (Magnaporthe grisea) specifically elicited the accumulation of JIOsPR10 mRNA in rice leaves (Jwa et al 2001). In Lithospermum erythrorhizon, pathogen infection with bacteria (Erwinia stewartii and Pseudomonas syringae) and fungi (Aspergillus nidulans and Penicillium funiculosum) strongly induced LePR1 transcripts in inoculated leaves.…”

Section: Discussionmentioning

confidence: 99%

Isolation and expression analysis of a novel pathogenesis-related protein 10 gene from Chinese wild Vitis pseudoreticulata induced by Uncinula necator

et al. 2010

Biologia

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A novel PR10 gene (designated as VpPR10) was isolated from Chinese wild Vitis pseudoreticulata W. T. Wang Baihe-35-1. VpPR10 encoded a 159-amino-acid polypeptide with a predicted molecular mass of 17.46 kDa. Sequence alignment showed that VpPR10 had high similarity with the PR10 proteins of other plant species. Genomic DNA gel blot analysis indicated that VpPR10 belonged to a multi-gene family in Chinese wild Vitis. Quantitative real-time PCR analysis exhibited that the transcript level of VpPR10 altered at different times after inoculation with Uncinula necator under natural field conditions. VpPR10 expression began to decrease after 24 h and reached the lowest level after 72 h. Then it began to increase and obtained the normal level after 96 h. After expression in Escherichia coli and purification, recombinant VpPR10 protein was used to immune rabbit to obtain polyclonal antibodies. Immunoblot analysis showed that the expression pattern of VpPR10 protein was similar with that of VpPR10 mRNA.

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“…Accumulations of JIOPR10 [73] and OsPR10 [74] transcripts were observed on application of JA and SA in rice leaves. The folding canon of PR-10 proteins is found in the ABA receptor family known as PYR/PYL/RCAR (pyrabactin resistance/PYR-like/regulatory component of ABA response) [21].…”

Section: Signaling Nodes: Pr-10 In Response To Signaling Pathwaysmentioning

confidence: 97%

The Pathogenesis Related Class 10 proteins in Plant Defense against Biotic and Abiotic Stresses

Jain¹

2015

APAR

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One of the most represented group of Pathogenesis Related (PR) genes are those of the PR-10 class. PR-10 proteins are members of multi genic family, and they often occur in clusters at specific loci following gene duplication and amplification events. To date, large number of PR-10 genes have been cloned and characterized in different species in response to abiotic and biotic stress. This review is focused on recent studies that have described the role, distribution and structure of PR-10 genes in plant genomes. Recent findings have provided insights into the functional roles of PR-10 proteins as ribonuclease, as cytokinin-specific binding proteins, a mammalian lipid transport and plant abscisic acid (ABA) receptor proteins, or as enzyme, (S)-norco claurine synthase. PR-10 proteins are differentially expressed in the presence of different signaling molecules, biotic stresses such as fungal, viral and bacterial pathogens and a number of abiotic stresses. The possibility to use this knowledge for genetic improvement of plant resistance to pathogens through classical breeding approach or transgenic technology is discussed.

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Molecular Cloning and Characterization of a Novel Jasmonate Inducible Pathogenesis-Related Class 10 Protein Gene, JIOsPR10, from Rice (Oryza sativa L.) Seedling Leaves

Cited by 141 publications

References 43 publications

Abscisic Acid-Induced Resistance against the Brown Spot Pathogen Cochliobolus miyabeanus in Rice Involves MAP Kinase-Mediated Repression of Ethylene Signaling

Abscisic Acid-Induced Resistance against the Brown Spot Pathogen Cochliobolus miyabeanus in Rice Involves MAP Kinase-Mediated Repression of Ethylene Signaling

Isolation and expression analysis of a novel pathogenesis-related protein 10 gene from Chinese wild Vitis pseudoreticulata induced by Uncinula necator

The Pathogenesis Related Class 10 proteins in Plant Defense against Biotic and Abiotic Stresses

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