Molecular cloning and sequence analysis of the cDNA encoding human apolipoprotein H (β2-Glycoprotein I)*

Day, Joseph R.; O׳Hara, Patrick J.; Grant, Francis James; Lofton–Day, Catherine; Berkaw, Mary N.; Werner, Philipp; Arnaúd, Philippe

doi:10.1007/bf02591656

Cited by 24 publications

(12 citation statements)

References 73 publications

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“…Although hepatocytes represent the main site of β 2 ‐GPI synthesis [9], the presence of β 2 ‐GPI mRNA has been demonstrated, by RT‐PCR, in fetal astrocytes, in cells from intestine and placenta [10,11]. More recently, we also detected β 2 ‐GPI mRNA in endothelial cells, lymphocytes, astrocytes and neurones [12].…”

Section: Introductionmentioning

confidence: 62%

Beta-2-glycoprotein I expression on monocytes is increased in anti-phospholipid antibody syndrome and correlates with tissue factor expression

Conti¹,

Sorice²,

Circella³

et al. 2003

Clinical and Experimental Immunology

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SUMMARYIt is well known that monocytes may play an active role in thrombogenesis, since they may express on their surface tissue factor, the major initiator of the clotting cascade. The results of this investigation demonstrate beta-2-glycoprotein I ( b 2 -GPI) mRNA expression by human peripheral blood monocytes, indicating that these cells synthesize b 2 -GPI. In addition, we show b 2 -GPI expression on cell surface of these cells by flow cytometric analysis, and the presence of this protein in cell lysate by Western blot. Interestingly, b 2 -GPI expression on monocytes is significantly increased in patients with antiphospholipid syndrome (APS) or systemic lupus erythematosus (SLE) as against healthy blood donors and correlates with tissue factor expression on monocytes. These findings support the view that monocytes are able to synthesize b 2 -GPI and suggest that patients with APS may have increased b 2 -GPI exposure on cell surface, which leads to persistently high monocyte tissue factor expression and consequently to a prothrombotic diathesis.

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Section: Introductionmentioning

confidence: 62%

Beta-2-glycoprotein I expression on monocytes is increased in anti-phospholipid antibody syndrome and correlates with tissue factor expression

Conti¹,

Sorice²,

Circella³

et al. 2003

Clinical and Experimental Immunology

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“…To shed light on this issue, we analyzed several normal rat tissues by different techniques. We began our studies on ApoH expression by performing Northern blot analysis on total RNAs, using a human apoH cDNA probe: this approach confirmed that the liver is the major site of biosynthesis for apoH (37).…”

Section: Discussionmentioning

confidence: 96%

“…The expression of apoH mRNA was initially detected by Northern blotting in human, rat and mouse liver and in two liver-derived cell lines, HepG2 and Hep3B (30,37,38). More recently apoH mRNA was found by RT-PCR on RNA extracted from other tissues (intestine, placenta, foetal astrocytes, lymphocytes) or cultured cells (transformed cell lines) such as choriocarcinoma, hepatoma (36), colon adenocarcinoma (35), neuroblastoma, umbilical vein endothelial cells (HUVEC, but not for Alvarado-de la Barrera, 39), astrocytoma, glioblastoma cells (40) and in monocytes (41).…”

Section: Introductionmentioning

confidence: 99%

RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues

Ragusa

Costa²,

Cefalù³

et al. 2006

Int J Mol Med

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Abstract. In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.

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“…ApoH, also known as β2-glycoprotein-I, is secreted from the liver [35] and exists in plasma as both free ApoH and bound to lipoprotein particles [36]. In the present study, ApoH levels were decreased in plasma from subjects with T2DM + high EIR compared with subjects with NGT.…”

Section: Figure 1 Plasma Protein Levels In Subjects With Ngt (White Bmentioning

confidence: 76%

Plasma proteome changes in subjects with Type 2 diabetes mellitus with a low or high early insulin response

et al. 2008

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Circulating proteins contribute to the pathogenesis of T2DM (Type 2 diabetes mellitus) in various ways. The aim of the present study was to investigate variations in plasma protein levels in subjects with T2DM and differences in beta-cell function, characterized by the EIR (early insulin response), and to compare these protein levels with those observed in individuals with NGT (normal glucose tolerance). Ten subjects with NGT+high EIR, ten with T2DM+high EIR, and ten with T2DM+low EIR were selected from the community-based ULSAM (Uppsala Longitudinal Study of Adult Men) cohort. Plasma protein profiling was performed using SELDI-TOF (surface-enhanced laser-desorption ionization-time-of-flight) MS. In total, nine plasma proteins differed between the three study groups (P<0.05, as determined by ANOVA). The levels of two forms of transthyretin, haemoglobin alpha-chain and haemoglobin beta-chain were decreased in plasma from subjects with T2DM compared with subjects with NGT, irrespective of the EIR of the subjects. Apolipoprotein H was decreased in plasma from individuals with T2DM+high EIR compared with subjects with NGT. Four additional unidentified plasma proteins also varied in different ways between the experimental groups. In conclusion, the proteins detected in the present study may be related to the development of beta-cell dysfunction.

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Molecular cloning and sequence analysis of the cDNA encoding human apolipoprotein H (β2-Glycoprotein I)*

Cited by 24 publications

References 73 publications

Beta-2-glycoprotein I expression on monocytes is increased in anti-phospholipid antibody syndrome and correlates with tissue factor expression

Beta-2-glycoprotein I expression on monocytes is increased in anti-phospholipid antibody syndrome and correlates with tissue factor expression

RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues

Plasma proteome changes in subjects with Type 2 diabetes mellitus with a low or high early insulin response

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