Molecular Cloning, Characterization, and Differential Expression of a Farnesyl-Diphosphate Synthase Gene from the Basidiomycetous FungusGanoderma lucidum

Abstract

A farnesyl-diphosphate synthase gene, designated GlFPS, was isolated from a triterpene-producing basidiomycetous fungus, Ganoderma lucidum. The GlFPS cDNA was found to contain an open reading frame of 1,083 bp, encoding a protein of 360 amino acids with a calculated molecular mass of 41.27 kDa. The deduced amino acid sequence of the GlFPS cDNA exhibited a high homology with other fungal FPS genes, and contained four conserved domains. Phylogenetic analysis showed that GlFPS belonged to the basidiomycete FPS group. Competitive PCR revealed that GlFPS was constitutively expressed in the mycelium growth stage, whereas the transcripts of GlFPS accumulated to high levels rapidly during the process of mushroom primordia. Treatment of mycelia with exogenous methyl jasmonate also caused a large accumulation of GlFPS mRNA. Subsequently, promoter analysis indicated that the 5' upstream region of GlFPS possessed various potential regulatory elements associated with physiological and environmental factors. Functional complementation of GlFPS in an ERG20-disrupted yeast strain indicated that the cloned cDNA encoded a farnesyl-diphosphate synthase.

Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase inAntrodia cinnamomea

“…In 2014, the triterpenoid biosynthesis pathway in A. cinnamomea was validated through genomic and transcriptome analyses; this pathway is similar to the MVA pathway in Ganoderma lucidum [13]. Several enzymes, such as squalene synthase (SQS) [14], HMG-CoA reductase (HMGR) [15], mevalonate pyrophosphate decarboxylase (MVD) [16], and farnesyldiphosphate synthase (FPS) [17], are implicated in the MVA pathway. A previous study showed that the synthesis of triterpenoids in A. cinnamomea is complex.…”

Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum

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Improved ganoderic acids production in Ganoderma lucidum by wood decaying components

“…Previous studies also proved that GAs yield was greatly influenced by a number of environmental factors including the elicitor, parameters of fermentation process and culture medium21464748.…”