Molecular cloning, mRNA expression and functional characterization of a catalase from Chinese black sleeper (Bostrychus sinensis)

Shen, Bin; Wei, Ke; Ding, Yuehan; Zhang, Jianshe

doi:10.1016/j.fsi.2020.04.056

Cited by 10 publications

(10 citation statements)

References 72 publications

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“…Moreover, the AdCAT protein sequence contains several conserved sequences and characteristic motifs, such as a proximal active site signature domain, a peroxisome targeting signal, and a heme-ligand signature motif, representing the typical characteristics of CAT family proteins. These findings were in line with several previous reports on CATs [19,24,38], strongly suggesting that AdCAT has similar functions to CATs from other species. The phylogenetic analysis demonstrated that AdCAT formed a clade with CATs from other teleosts, and was closely related to CAT from sterlet sturgeon.…”

Section: Discussionsupporting

confidence: 93%

“…This was consistent with earlier research on Pengze crucian carp [19]. To date, many studies have revealed that the liver is a vital organ for the expression of antioxidant genes in teleosts [24,[38][39][40]. The high expression of the antioxidant genes might be related to the fact that the liver is the key organ in which oxidative reactions and antioxidant defenses occur [39].…”

Section: Discussionsupporting

confidence: 91%

“…Pengze [19], marbled eel, Anguilla marmorata [20], and large yellow croaker, Pseudosciaena crocea [21]. CAT gene sequences have been reported for turbot, Scophthalmus maximus [22], rock bream, Oplegnathus fasciatus [23], and Chinese black sleeper, Bostrychus sinensis [24]. So far, the Cu/Zn SOD and CAT from Yangtze sturgeon have not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Cu/Zn Superoxide Dismutase and Catalase of Yangtze Sturgeon, Acipenser dabryanus: Molecular Cloning, Tissue Distribution and Response to Fasting and Refeeding

Shi

Xiong

Wen

et al. 2022

Fishes

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Superoxide dismutase and catalase are two major antioxidant enzymes in the fish antioxidant defense system, which can remove excess reactive oxygen species and protect fish from stress-induced oxidative damage. The present study aimed to clone the sequences of Yangtze sturgeon, Acipenser dabryanus, Cu/Zn superoxide dismutase (AdCu/Zn-SOD) and catalase (AdCAT), and to explore changes of gene expression in the liver and intestine during fasting and refeeding. A total of 120 fish were exposed to four fasting and refeeding protocols (fasting for 0, 3, 7, or 14 d and then refeeding for 14 d). The coding sequences of AdCu/Zn-SOD and AdCAT encoded 155 and 526 amino acid proteins, respectively, both of which were expressed mainly in the liver. During fasting, when compared to the control group, liver AdCu/Zn-SOD expression was significantly higher in the 3- and 14-d groups, whereas its intestinal expression increased significantly only in the 7-d group. Liver AdCAT expression increased significantly in the 3-, 7-, and 14-d groups. During refeeding, liver AdCu/Zn-SOD expression increased significantly in the 3-, 7-, and 14-d groups compared with those in the control group. Similarly, intestinal AdCu/Zn-SOD expression increased significantly in the 3- and 7-d groups. Moreover, intestinal AdCAT expression was significantly higher in the 3-d group than in the control group, but decreased significantly in the 14-d group. Our findings indicated that AdCu/Zn-SOD and AdCAT play important roles in protecting fish against starvation-induced oxidative stress. Yangtze sturgeon exhibited the potential to adapt to a starvation and refeeding regime.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Cu/Zn Superoxide Dismutase and Catalase of Yangtze Sturgeon, Acipenser dabryanus: Molecular Cloning, Tissue Distribution and Response to Fasting and Refeeding

Shi

Xiong

Wen

et al. 2022

Fishes

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“…In the present study, PIC induced Hdh-CAT mRNA expression levels in the gill and DG tissues of Pacific abalone. PIC can also induce mRNA expression of CAT in the liver (DG) of Bostrychus sinensis [73]. Upregulated mRNA expression of CAT in viral challenged gill of H. discus discus has been previously reported [28].…”

Section: Discussionmentioning

confidence: 93%

Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone

et al. 2023

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Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (Hdh-CAT) in stress physiology, innate immunity, testicular development, metamorphosis, and cryopreserved sperm of Pacific abalone. Hdh-CAT gene was cloned from the digestive gland (DG) of Pacific abalone. The 2894 bp sequence of Hdh-CAT had an open reading frame of 1506 bp encoding 501 deduced amino acids. Fluorescence in situ hybridization confirmed Hdh-CAT localization in the digestive tubules of the DG. Hdh-CAT was induced by different types of stress including thermal stress, H2O2 induction, and starvation. Immune challenges with Vibrio, lipopolysaccharides, and polyinosinic–polycytidylic acid sodium salt also upregulated Hdh-CAT mRNA expression and catalase activity. Hdh-CAT responded to cadmium induced-toxicity by increasing mRNA expression and catalase activity. Elevated seasonal temperature also altered Hdh-CAT mRNA expression. Hdh-CAT mRNA expression was relatively higher at the trochophore larvae stage of metamorphosis. Cryopreserved sperm showed significantly lower Hdh-CAT mRNA expression levels compared with fresh sperm. Hdh-CAT mRNA expression showed a relationship with the production of ROS. These results suggest that Hdh-CAT might play a role in stress physiology, innate immunity, testicular development, metamorphosis, and sperm cryo-tolerance of Pacific abalone.

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“…Therefore, it is essential to modify a protein to make it more suitable for acid environments. A previous study observed that rCAT from Chinese black sleeper (Bostrychus sinensis) participated in the oxidative homeostasis of the sleeper during pathogen intrusion [28]. Shaeer et al found that a recombinant manganese-catalase from Geobacillus thermopakistaniensis had high activity and heat resistance, making it a promising candidate for industrial applications [18].…”

Section: Degradation Of Afm 1 In Milk and Beermentioning

confidence: 99%

Degradation Mechanism of Aflatoxin M1 by Recombinant Catalase from Bacillus pumilusE-1-1-1: Food Applications in Milk and Beer

Liu,

Zhao,

Wang

et al. 2024

Foods

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A bacteria capable of degrading aflatoxin M1 (AFM1) was isolated from African elephant manure. It was identified as Bacillus pumilus by 16s rDNA sequencing and named B. pumilusE-1-1-1. Compared with physical and chemical methods, biological methods have attracted much attention due to their advantages, such as thorough detoxification, high specificity, and environmental friendliness. This work aimed to study the effects of a recombinant catalase (rCAT) from B. pumilusE-1-1-1 on the degradation of AFM1 in pattern solution. The degradation mechanism was further explored and applied to milk and beer. Kinetic Momentum and Virtual Machine Maximum values for rCAT toward AFM1 were 4.1 μg/mL and 2.5 μg/mL/min, respectively. The rCAT-mediated AFM1 degradation product was identified as C15H14O3. Molecular docking simulations suggested that hydrogen and pi bonds played major roles in the steadiness of AFM1–rCAT. In other work, compared with identical density of AFM1, survival rates of Hep-G2 cells incubated with catalase-produced AFM1 degradation products increased by about 3 times. In addition, degradation rates in lager beer and milk were 31.3% and 47.2%, respectively. Therefore, CAT may be a prospective substitute to decrease AFM1 contamination in pattern solution, milk, and beer, thereby minimizing its influence on human health.

show abstract

Molecular cloning, mRNA expression and functional characterization of a catalase from Chinese black sleeper (Bostrychus sinensis)

Cited by 10 publications

References 72 publications

Cu/Zn Superoxide Dismutase and Catalase of Yangtze Sturgeon, Acipenser dabryanus: Molecular Cloning, Tissue Distribution and Response to Fasting and Refeeding

Cu/Zn Superoxide Dismutase and Catalase of Yangtze Sturgeon, Acipenser dabryanus: Molecular Cloning, Tissue Distribution and Response to Fasting and Refeeding

Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone

Degradation Mechanism of Aflatoxin M1 by Recombinant Catalase from Bacillus pumilusE-1-1-1: Food Applications in Milk and Beer

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