Molecular cloning of cDNAs encoding insecticidal neurotoxic peptides from the spider Phoneutria nigriventer

Penaforte, C.L.; Prado, Vânia F.; Romano-Silva, Marco Aurélio; Guimarães, Pedro Edson Moreira; Marco, Luiz; Gomez, Marcus V.; Kalapothakis, Evanguedes

doi:10.1016/s0041-0101(99)00237-8

Cited by 35 publications

(18 citation statements)

References 19 publications

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“…Symptoms induced by the recombinant GlyTX4 toxin in S. frugiperda were identical to those described for the native TX4(6-1) venom peptide isolated from P. nigriventer (13,35) consisting of a lack of coordination, disorientation and uncontrolled movement. The results obtained with GlyTX4 expressed in E. coli and the studies with ω-ACTX-Hv1a toxin (Hvt) expressed in transgenic plants (26) showed that the mode of action of the heterologously expressed spider peptides are similar.…”

Section: J E F Figueiredo Et Al Heterologous Expression Of An Inssupporting

confidence: 55%

“…A cDNA clone [TX4(6-1)] encoding a precursor toxin with insecticidal activity was previously isolated from P. nigriventer venom gland cDNA library (35). The TX4 clone was used as template to amplify the coding region for the active peptide by value was highly significant (18,739) and p=0.998.…”

Section: Methodsmentioning

confidence: 99%

“…Numerous low molecular weight polypeptides with neurotoxic activity were identified in extracts from the venom glands of the South American armed spider Phoneutria nigriventer (13,16,35). The chromatographic fraction TX4(6-1) of P. nigriventer venom is specific against insects with no toxic effect to avian and mammalian species (35).…”

Section: Introductionmentioning

confidence: 99%

“…The chromatographic fraction TX4(6-1) of P. nigriventer venom is specific against insects with no toxic effect to avian and mammalian species (35). Tx4(6-1) toxin was highly active against house flies, producing neurotoxic effects at the low dose of 0.02ng/mg and against cockroaches, at doses ranging from 0.5 to 2.5ng/mg (35). Bioassay with Tx4(6-1) polypeptide against important maize crop insect-pests could lead to new technological insights for insectresistant plants in agriculture.…”

Section: Introductionmentioning

confidence: 99%

“…Many cDNA clones encoding P. nigriventer neuropeptides were isolated by screening a venom gland cDNA library (35). Computer analysis and experimental data revealed that a cDNA clone [Tx4(6-1)] encodes an inactive polypeptide with 82 amino acids with 16aa residues at N-terminus corresponding to the endoplasmic reticulum (ER) signal peptide.…”

Section: Introductionmentioning

confidence: 99%

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Heterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer)

Figueiredo¹,

Kalapothakis²,

Gomez³

et al. 2008

J. Venom. Anim. Toxins incl. Trop. Dis

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Insect-pests are global problems that cause severe damage to crop plants, and their control is commonly based on chemical insecticides. However, negative effects of pesticides on the environment and human health emphasize the necessity to develop alternative methods for insect-pest control. In the present study, a gene coding for the insecticidal peptide TX4(6-1) of the Brazilian armed spider (Phoneutria nigriventer) was cloned in fusion with maltose binding protein (MBP) and expressed in Escherichia coli. The affinity purified protein MBP-GlyTX4 was cleaved with the Xa factor and used for a bioassay against Spodoptera frugiperda and rabbit immunization. Five micrograms GlyTX4 protein injected into the hemocoel of larvae and abdominal cavity of adults produced trembling and uncoordinated movements immediately after injection and all adult insects died after 12h. After two days, larvae became paralyzed and the epidermal color changed to dark brown. Furthermore, the development stage was prolonged for two weeks. Alternatively, slices of maize leaves were imbibed with 15 micrograms of the recombinant protein cleaved with the Xa factor and used as diet for larvae. In this experiment, all larvae died in about 30 minutes. Polyclonal antibodies anti-MBP-GlyTX4 were effective for recognizing MBP and GlyTX4 in whole cell extract from E. coli expressing the recombinant protein.

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Section: J E F Figueiredo Et Al Heterologous Expression Of An Inssupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Heterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer)

Figueiredo¹,

Kalapothakis²,

Gomez³

et al. 2008

J. Venom. Anim. Toxins incl. Trop. Dis

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Arthropod venoms: A vast arsenal of insecticidal neuropeptides

Schwartz¹,

Mourão²,

Moreira³

et al. 2012

Biopolymers

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Arthropods are the most diverse animal group on the planet, and occupy almost all ecological niches. Venomous arthropods are a rich source of bioactive compounds evolved for prey capture and defense against predators and/or microorganisms. These highly potent chemical arsenals represent an available source for new insecticidal compounds as they act selectively on their molecular targets. These toxins affect the invertebrate nervous system and, until the moment, several insecticidal compounds belonging to the class of peptides or polyamine-like compounds have been purified and characterized from the venom of arachnids and hymenopterans. This review focuses on invertebrate-specific peptide neurotoxins that have been isolated from the venom ofspiders, scorpions, centipedes, ants, and wasps, discussing their potential in pest control and as invaluable tools in neuropharmacology.

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Reducing Virus Associated Crop Loss Through Resistance to Insect Vectors

Mutschler

Wintermantel

Natural Resistance Mechanisms of Plants to Viruses

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Molecular cloning of cDNAs encoding insecticidal neurotoxic peptides from the spider Phoneutria nigriventer

Cited by 35 publications

References 19 publications

Heterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer)

Heterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer)

Arthropod venoms: A vast arsenal of insecticidal neuropeptides

Reducing Virus Associated Crop Loss Through Resistance to Insect Vectors

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