Molecular cloning of TaMATE2 homoeologues potentially related to aluminium tolerance in bread wheat (Triticum aestivum L.)

Abstract

Recently, members of the MATE family have been implicated in aluminium (Al) tolerance by facilitating citrate efflux in plants. The aim of the present work was to perform a molecular characterisation of the MATE2 gene in bread wheat. Here, we cloned a member of the MATE gene family in bread wheat and named it TaMATE2, which showed the typical secondary structure of MATE-type transporters maintaining all the 12 transmembrane domains. Amplification in Chinese Spring nulli-tetrasomic and ditelosomic lines revealed that TaMATE2 is located on the long arm of homoeologous group 1 chromosomes. The transcript expression of TaMATE2 homoeologues in two diverse bread wheat genotypes, Barbela 7/72/92 (Al-tolerant) and Anahuac (Al-sensitive), suggested that TaMATE2 is expressed in both root and shoot tissues of bread wheat, but mainly confined to root rather than shoot tissues. A time-course analysis of TaMATE2 homoeologue transcript expression revealed the Al responsive expression of TaMATE2 in root apices of the Al-tolerant genotype, Barbela 7/72/92. Considering the high similarity of TaMATE2 together with similar Al responsive expression pattern as of ScFRDL2 from rye and OsFRDL2 from rice, it is likely that TaMATE2 also encodes a citrate transporter. Furthermore, the TaMATE2-D homoeologue appears to be near the previously reported locus (wPt0077) on chromosome 1D for Al tolerance. In conclusion, molecular cloning of TaMATE2 homoeologues, particularly TaMATE2-D, provides a plausible candidate for Al tolerance in bread wheat that can be used for the development of more Al-tolerant cultivars in this staple crop.

Genome-Wide Identification of MATE Gene Family in Potato (Solanum tuberosum L.) and Expression Analysis in Heavy Metal Stress

“…Similar results were found for DTX/MATE protein in flax ( Ali et al, 2020 ). As phytohormones are very crucial for MATE proteins to extrude heavy metal and transmit certain disease resistance signals ( Serrano et al, 2013 ; Garcia-Oliveira et al, 2018 ; Tegli et al, 2020 ). We selected 13 cis-acting elements, based on their functional annotations, and found that 61.54% (8/13) of them were also related to hormone response ( Figure 6 ).…”

Genome‐wide identification and expression analysis of detoxification efflux carriers (DTX) genes family under abiotic stresses in flax

“…5 in mouse, 11 in human), plant genomes encode a large number of MATE genes, for instance, 56 in Arabidopsis thaliana (Li et al 2002), 53 in Oryza sativa (Tiwari et al 2014), 70 in Medicago truncatula (Zhao and Dixon 2009), 128 in Gossypium hirsutum (Lu et al 2019), 49 in Zea mays (Zhu et al 2016), 48 in potato (Li et al 2019), 67 in tomato (dos Santos et al 2017), 67 in Pigeon pea (Dong et al 2019), 71 in Populus trichocarpa (Li et al 2017) and 117 in soybean (Liu et al 2016). Besides, MATE gene family has also been identified in other plant species such as Sorghum bicolor (Sivaguru et al 2013), wheat (Garcia‐Oliveira et al 2018), Setaria viridis (Ribeiro et al 2017), persimmon (Yang et al 2016), cabbage (Wu et al 2014), Nicotiana tabacum (Morita et al 2009) and Lotus japonicus (Takanashi et al 2013), but no work has been reported in flax ( Linum usitatissimum ) so far.…”

Mapping QTLs of flag leaf morphological and physiological traits related to aluminum tolerance in wheat (Triticum aestivum L.)

“…The major QFLL-1D-a.NO16, QFLW-1D-a.NO15, QGYLD-1A.AL15, and the putative QCMS-1A.AL16 were determined by the CIM method, likewise the stable QTLs for FLL (on LG 1A), CMS (on LG 1B), and GYLD and Al (on LG 1D-a) were determined by the MCIM method and presented in our study. These QTLs were probably associated with the TaMATE2 gene located on the long arm of homoeologous group 1 chromosomes (1A, 1B, and 1D) in bread wheat, which encodes a citrate transporter and increased Al tolerance (Garcia-Oliveira et al 2018). The efflux of malate anions from the root apices, which typifies an important mechanism for Al tolerance, is encoded by TaALMT1, a gene located on chromosome 4DL (Navakode et al 2014).…”