Molecular cloning of partial cDNA copies of two distinct mouse IFN-β mRNAs

Abstract

Two cDNA libraries were constructed, using respectively the 12S and the 16S sucrose gradient fractions of polysomal poly (A)+ RNA from mouse C243 cells induced with Newcastle disease virus. Screening of a part of both libraries by mRNA selection hybridization assays revealed the presence of two plasmids hybridizing to an mRNA, whose translation product was characterized as mouse IFN-beta. Blot analysis of RNA indicated that mRNA hybridizing to the DNA from both plasmids could be detected in induced but not in uninduced C243 cells. The two cDNA inserts did not cross hybridize and had distinct restriction maps. Sequencing revealed that both inserts represented the end of the coding region and the entire 3' non coding region of two district mRNAs. Although different, the putative 39 AA and 65 AA carboxy termini of both Mu IFN-beta s display some homology to human IFN-beta 1. Thus there are at least two different murine IFN-beta genes.

Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) Is Differentially Induced in Neurons and Astrocytes after Seizures: Evidence for Developmental, Immediate Early Gene, and Lesion Response

“…This probe recognized only one band where size corresponds to the TIMP-1 mRNA on Northern blots with brain RNA (Nedivi et al, 1993). Because a 150 bp sequence of the TIMP-1 3Ј coding region is highly homologous to a sequence contained in murine ␤-interferon (Skup et al, 1982), control experiments were performed with a 35 S-dATP-labeled oligonucleotide selected in a region of the rat TIMP-1 (5Ј 79 -123 3Ј) nonhomologous to ␤-interferon. The in situ hybridization results obtained with such an oligoprobe were identical to those obtained with the TIMP-1 riboprobe in both control and KA-treated animals.…”

Granulocyte-macrophage colony-stimulating factor and tetradecanoyl phorbol acetate induce a distinct, restricted subset of primary-response TIS genes in both proliferating and terminally differentiated myeloid cells.

“…We have recently completed the sequence of the TIS1 cDNA (data not shown). This cDNA has also been cloned as the NGFinducible sequence NGF1B (20) cloned as a Newcastle disease virus-inducible message (23). A rat homolog, PC-4, has also been cloned from an NGFinduced PC12 library (26).…”

A growth-responsive gene (16C8) in normal mouse fibroblasts homologous to a human collagenase inhibitor with erythroid-potentiating activity: evidence for inducible and constitutive transcripts

“…The deduced amino acid sequence of the Mr 22,500 translation product reveals an N-terminal hydrophobic region consistent with the possibility that the 16C8 product is a secreted, glycosylated protein. Comparison with published sequences disclosed that the 3' end of the cDNA clone was virtually identical to the 3' end of a putative murine beta-interferon cDNA clone (20). Gewert, Coloumbe, Castelino, Skup and Williams (manuscript submitted) have sequenced a large part of a genomic clone corresponding to this cDNA and have concluded that it is not a beta-interferon but rather the murine equivalent of a human cDNA encoding erythroid-potentiating activity (EPA, 21) and tissue inhibitor of metalloproteinases (TIMP, 22); they, like us, have observed substantial homologies at the amino acid and nucleotide sequence levels and, further, have demonstrated that the encoded murine protein can inhibit collagenase.…”

“…In this paper, we report the nucleotide sequence 6f a nominally full length cDNA clone of a characteristic "late" gene, 16C8. The sequence of the 3' terminal region is identical to the sequence of part of a cDNA encoding a putative 6-interferon (20). We also present some studies on the structure of the mRNA and discuss the nature of the encoded protein, which appears to be the murine equivalent of a protein secreted by human cells that apparently has both erythroidpotentiating activity (EPA, 21) and collagenase-inhibitor activity (TIMP, tissue inhibitor of metalloproteinase, 22).…”