Molecular cytogenetic identification of a wheat–rye 1R addition line with multiple spikelets and resistance to powdery mildew

Yang, Wujuan; Wang, Changyou; Chen, Chunhuan; Wang, Yajuan; Zhang, Hong; Liu, Xinlun; Ji, Wanquan

doi:10.1139/gen-2015-0129

Cited by 20 publications

(11 citation statements)

References 49 publications

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“…These kinds of perturbations of gene networks can lead to positive results. For example, the wheat-rye 1BL•1RS translocation line conferred superior genes, including those for biotic and abiotic stress tolerance, high yield potential, and wide adaptability to different environments, which contribute to worldwide production [37–41]. The wheat-rye 4R and 6R chromosome translocation lines showed strong resistance to powdery mildew [42, 43].…”

Section: Discussionmentioning

confidence: 99%

The genome-wide transcriptional consequences of the nullisomic-tetrasomic stocks for homoeologous group 7 in bread wheat

Zhang¹,

Geng

Qin

et al. 2019

BMC Genomics

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BackgroundHexaploid bread wheat (Triticum aestivum L) arose by two polyploidisation events from three diploid species with homoeologous genomes. Nullisomic-tetrasomic (nulli-tetra or NT) lines are aneuploid wheat plants lacking two and adding two of six homoeologous chromosomes. These plants can grow normally, but with significantly morphological variations because the adding two chromosomes or the remaining four chromosomes compensate for those absent. Despite these interesting phenomena, detailed molecular mechanisms underlying dosage deletion and compensation in these useful genetic materials have not been determined.ResultsBy sequencing the transcriptomes of leaves in two-week-old seedlings, we showed that the profiles of differentially expressed genes between NT stocks for homoeologous group 7 and the parent hexaploid Chinese Spring (CS) occurred throughout the whole genome with a subgenome and chromosome preference. The deletion effect of nulli-chromosomes was compensated partly by the tetra-chromosomes via the dose level of expressed genes, according to the types of homoeologous genes. The functions of differentially regulated genes primarily focused on carbon metabolic process, photosynthesis process, hormone metabolism, and responding to stimulus, and etc., which might be related to the defective phenotypes that included reductions in plant height, flag leaf length, spikelet number, and kernels per spike.ConclusionsThe perturbation of the expression levels of transcriptional genes among the NT stocks for homoeologous group 7 demonstrated the gene dosage effect of the subgenome at the genome-wide level. The gene dosage deletion and compensation can be used as a model to elucidate the functions of the subgenomes in modern polyploid plants.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5421-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

The genome-wide transcriptional consequences of the nullisomic-tetrasomic stocks for homoeologous group 7 in bread wheat

Zhang¹,

Geng

Qin

et al. 2019

BMC Genomics

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“…Spikes per acre, numbers of seeds per spike, and thousand seed weight are three factors affecting wheat yield (Yang et al . 2016).…”

Section: Resultsmentioning

confidence: 99%

A wheat-<i>Aegilops umbellulata</i> addition line improves wheat agronomic traits and processing quality

Jia

Yang

et al. 2019

Breed. Sci.

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Wheat processing quality is mainly correlated with high-molecular-weight glutenin subunits (HMW-GS) of grain endosperm. In bread wheat, the number of HMW-GS alleles are limited. However, wheat relative species possess numerous HMW-GS genes. In our previous study, a pair of novel HMW-GS 1Ux3.5+1Uy1.9 was characterized in Aegilops umbellulata. In this work, a novel wheat-Ae. umbellulata addition line, GN05, carrying a pair of 1U chromosome was developed and identified via cytogenetic analysis. Protein composition analysis indicated that GN05 carried HMW-GS of Ae. umbellulata. Accumulation of glutenin macropolymer (GMP) showed that GN05 had a much higher GMP content than the recurrent parent Chinese Spring. Rheological characteristics were analyzed by mixing test and the dough quality of GN05 was significantly improved compared to Chinese Spring. The results presented here may provide a valuable resource for the improvement of bread wheat quality.

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“…Black diamond shapes indicate no template control in wheat breeding. A great number of desirable wheatrye progenies from various rye accession have been developed by distant hybridization and chromosome engineering, such as wheat-rye 6R disomic addition line WR49 carrying powdery mildew resistance derived from rye cultivar German White [37], wheat-rye 1R disomic addition line N9436B carrying powdery mildew resistance derived from rye cultivar Austrian [38], and wheatrye T1RS•1BL translocation lines from Chinese rye cultivar Baili with resistances to stripe rust and powdery mildew and desirable agronomic traits [39]. Compared with addition lines and substitution lines, translocation lines, especially small segment translocation lines carrying desirable traits, are preferred attributing to less linkage drag.…”

Section: Discussionmentioning

confidence: 99%

Scale development and utilization of universal PCR-based and high-throughput KASP markers specific for chromosome arms of rye (Secale cereale L.)

et al. 2020

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Background: Rye (Secale cereale L., 2n = 2x = 14, RR), a relative of common wheat, is a large gene resource pool for wheat improvement. Accurate and convenient identification of the rye chromatin in wheat background will facilitate the transfer and utilization of elite genes derived from rye in wheat breeding. Results: In the present study, five rye cultivars including Imperial, German White, Jingzhouheimai, Baili and Guyuan were sequenced by specific-locus amplified fragment sequencing (SLAF-seq) to develop large-scale rye-specific markers. Based on SLAF-seq and bioinformatics analyses, a total of 404 universal PCR-based and a whole set of Kompetitive allele-specific PCR (KASP) markers specific for the 14 individual rye chromosome arms were developed and validated. Additionally, two KASP markers specific for 1RS and 2RL were successfully applied in the detection of 1RS translocations in a natural population and 2RL chromosome arms in wheat-rye derived progenies that conferred adult resistance to powdery mildew. Conclusion: The 404 PCR-based markers and 14 KASP markers specific for the 14 individual rye chromosome arms developed in this study can enrich the marker densities for gene mapping and accelerate the utilization of rye-derived genes in wheat improvement. Especially, the KASP markers achieved high-throughput and accurate detection of rye chromatin in wheat background, thus can be efficiently used in marker-assisted selection (MAS). Besides, the strategy of rye-specific PCR-based markers converting into KASP markers was high-efficient and low-cost, which will facilitate the tracing of alien genes, and can also be referred for other wheat relatives.

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Molecular cytogenetic identification of a wheat–rye 1R addition line with multiple spikelets and resistance to powdery mildew

Abstract: N9436B was cytological stable, had the trait of multiple spikelets and resistance to powdery mildew, and is expected to be useful in wheat improvement.

Cited by 20 publications

References 49 publications

The genome-wide transcriptional consequences of the nullisomic-tetrasomic stocks for homoeologous group 7 in bread wheat

The genome-wide transcriptional consequences of the nullisomic-tetrasomic stocks for homoeologous group 7 in bread wheat

A wheat-<i>Aegilops umbellulata</i> addition line improves wheat agronomic traits and processing quality

Scale development and utilization of universal PCR-based and high-throughput KASP markers specific for chromosome arms of rye (Secale cereale L.)

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