Molecular design of synthetic receptors with dynamic, imprinting, and allosteric functions

Shinkai, Seiji; Takeuchi, Masayuki

doi:10.1016/j.bios.2004.03.036

Cited by 57 publications

(17 citation statements)

References 55 publications

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“…Non‐covalent interactions (hydrogen bonds or ionic interactions) are easily disturbed in water. Thus, the covalent method has been the main approach for saccharides , . However, more chemicals and detailed optimisation of polymerisation and rebinding conditions are needed in this approach.…”

Section: Resultsmentioning

confidence: 99%

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Application of molecularly imprinted hydrogel for the preparation of lactose‐free milk

2012

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Section: Resultsmentioning

confidence: 99%

“…Thus, the covalent method has been the main approach for saccharides. 31,32 However, more chemicals and detailed optimisation of polymerisation and rebinding conditions are needed in this approach. In this study, lactose imprinted hydrogels were prepared by non-covalent imprinting.…”

Section: Preparation Of Molecularly Imprinted Polymers For Lactosementioning

confidence: 99%

Application of molecularly imprinted hydrogel for the preparation of lactose‐free milk

2012

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“…If h = 1 the individual binding events are non-cooperative. [26,28,31,[34][35][36] Since most of these are at least about 10 years old and almost all cover only certain aspects of this field the aim of this review is to give a more comprehensive overview of artificial homotropic and heterotropic allosteric receptors where the interaction of a substrate with a synthetic receptor is influenced by a conformational change upon binding of an effector. [22,23] A number of supramolecular systems based on the concept of allosteric effects have been established since the first examples mentioned above and the subject has been issue of some reviews [24][25][26][27][28][29][30][31][32][33][34][35][36] already although not all of them were really focused on allosteric effects.…”

Section: Introductionmentioning

confidence: 99%

Artificial Allosteric Receptors

Kremer¹,

Lützen²

2013

Chemistry A European J

132

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Cooperative effects in the binding of two or more substrates to different binding sites of a receptor that are a result of a conformational change caused by the binding of the first substrate--also referred to as the effector--are called allosteric effects. In biological systems, allosteric regulation is a widely used mechanism to control the function of proteins and enzymes in cellular metabolism. Inspired by this a lot of efforts have been made in supramolecular chemistry to implement this concept into artificial systems to control functions as molecular recognition, signal amplification, or even reactivity and catalysis. This review gives an up-to-date overview over the different approaches that have been reported ever since the first examples from the late 1970s/early 1980s. It covers both homo- and heterotropic examples and is divided according to the nature of the effector--cationic, anionic, or neutral--effectors and systems that use combinations of those.

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“…Fluorescent functional monomers have been investigated for the imprinting of a variety of small molecules, where fluorescence intensity alters upon a specific binding event or template-cavity interaction (5)(6)(7)(8)(9) . Fluorescent detection of small organic template molecules has been extensively employed in different forms (10,11) , and hydrogels have been imaged using confocal microscopy and employed in a variety of differing functional roles (12)(13)(14) .…”

Section: Introductionmentioning

confidence: 99%

Quantification and Confocal Imaging of Protein Specific Molecularly Imprinted Polymers

et al. 2006

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We have employed FITC-albumin as the protein template molecule in an aqueous phase molecular imprinted polymer (HydroMIP) strategy. For the first time, the use of a fluorescently labelled template is reported, with subsequent characterisation of the smart material to show that the HydroMIP possess a significant molecular memory in comparison to that of the nonimprinted control polymer (HydroNIP). The imaging of the FITC-albumin imprinted HydroMIP using confocal microscopy is described, with the in situ removal of imprinted protein displayed in terms of observed changes in the fluorescence of the imprinted polymer, both before and after template elution (using a 10% SDS/10% AcOH (w/v) solution). We also report the imaging of a bovine haemoglobin (BHb) imprinted HydroMIP using two-photon confocal microscopy, and describe the effects of template elution upon protein autofluorescence. The findings further contribute to the understanding of aqueous phase molecular imprinting protocols, and document the use of fluorescence as a useful tool in template labelling/detection and novel imaging strategies.

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Molecular design of synthetic receptors with dynamic, imprinting, and allosteric functions

Cited by 57 publications

References 55 publications

Application of molecularly imprinted hydrogel for the preparation of lactose‐free milk

Application of molecularly imprinted hydrogel for the preparation of lactose‐free milk

Artificial Allosteric Receptors

Quantification and Confocal Imaging of Protein Specific Molecularly Imprinted Polymers

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