Molecular detection and phylogenetic analysis of Ehrlichia canis in a Philippine dog

Maekawa, Naoya; Konnai, Satoru; Balbin, Michelle M.; Mingala, Claro N.; Gicana, Karlo Romano B.; Bernando, Francis A.E.M.; Shiro, Motoo; Ohashi, Kazuhiko

doi:10.1016/j.ttbdis.2017.09.012

Cited by 5 publications

(7 citation statements)

References 14 publications

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“…Aguiar [9]. Maekawa et al [7] confirmed significant differences between E. canis strains in the Philippine region and the US and Brazil strains. Furthermore, Nambooppha et al [14] found two gene clusters in the local strain of E. canis in the local dog population in Thailand, the USA strain, and the Taiwan strain.…”

Section: Discussionmentioning

confidence: 89%

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Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

et al. 2020

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Background and Aim: As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials and Methods: A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene. Results: The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic analysis of E. canis gp36 protein, these E. canis strains in different geographical regions of the world can be divided into Genogroup I and Genogroup II. Among them, the Xinjiang epidemic strain XJ-6 and 533, 36, 1055, Kasur1, and Jake strains were clustered into subgroup 1.1 of Genogroup I, while the XJ-2, XJ-21, and XJ-35 strains and the TWN1, TWN4, CM180, and CM196 strains were closely related and belonged to subgroup 2.2 of Genogroup II, displaying high genetic diversity. Conclusion: This is the first study focusing on the molecular epidemiology of E. canis infection in pet dogs, which revealed that E. canis infection had been occurred in Xinjiang, China. More importantly, this study confirmed that the substantial variability in immunoreactive protein gp36 from E. canis strains circulating in pet dogs.

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Section: Discussionmentioning

confidence: 89%

“…Since E. canis was first detected in Algeria in 1935, it has been found in dogs and ticks in North America, Europe, Asia, and Africa. At present, CME is spreading all over the world [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

et al. 2020

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“…Todos os achados hematológicos encontrados, ainda que presentes na EMC, não apontam exclusivamente para esse tipo de doença, por serem inespecíficos e inconstantes, também podem ser indicativos de outras doenças, bem como de alterações fisiológicas transitórias relacionadas à fase de vida do sujeito 27 .…”

Section: Resultsunclassified

Detection and Laboratory Findings Due to Ehrlichia Canis in Dogs From the South Area of São Paulo – Sp, Brazil

Queiroz

Aguiar

Silva

et al. 2022

Braz. Jour. Global Health

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OBJECTIVE: To evaluate hematological changes in dogs diagnosed with Ehrlichia canis. METHODS: 54 dogs treated at the Veterinary Hospital of the Santo Amaro University were analyzed. During the consultation, the animals' real temperature was measured; and venous blood samples were collected for hemogram; and performed serological and molecular tests, through Immunofluorescence and PCR in real time, respectively. RESULTS: Among the 54 animals suspected of having E. canis, 32 (59.3%) were diagnosed as positive. Of these, 23 (42.6%) were positive only in the serological test, while 9 (16.7%) had positive results in the serological and molecular tests. Thrombocytopenia was the most important alteration found in this study and was the only parameter found in the blood count that showed a statistical difference. CONCLUSION: It was concluded that thrombocytopenia was the main finding in infected animals and that the combination of molecular and serological diagnoses may increase the chances of detection of infection or exposure to the agent.

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“…turanicus s.s. is a three-host tick and is characterized by constant host changes during development (42); this suggests that there is a possibility of pathogen transmission from domestic animals to pet dogs via ticks. Canine monocytic ehrlichiosis (CME), a pathogen caused by E. canis, has been found in dogs and ticks in North America, Europe, Asia, and Africa since it was first identified in Algeria in 1935 and is now spreading around the world (15,(43)(44)(45). One recent study in XUAR reported that the prevalence of E. canis in pet dogs was 12.12% (45), while another study did not detect Ehrlichia spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Zoonotic and Veterinary Pathogenic Bacteria in Pet Dogs and Their Parasitizing Ticks in Junggar Basin, North-Western China

et al. 2022

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Despite the recognized epidemiological importance of ticks as vectors for pathogens that cause numerous zoonotic and veterinary diseases, data regarding the pathogens of pet dogs and their parasitic ticks in the Junggar Basin are scarce. In this study, a total of 178 blood samples and 436 parasitic ticks were collected from pet dogs in Junggar Basin, Xinjiang Uygur Autonomous Region (XUAR), north-western China. All ticks were identified as Rhipicephalus turanicus sensu stricto (s.s.) according to morphological and molecular characteristics. Rh. turanicus s.s. ticks were collected from pet dogs in China for the first time. Seven tick-borne pathogens, such as Ehrlichia chaffeensis, Anaplasma phagocytophilum, Rickettsia massiliae, Candidatus R. barbariae, Brucella spp., Rickettsia sibirica, and Anaplasma ovis, were detected from ticks, whereas the first five bacteria were detected from blood samples of dogs. Brucella spp. was the most predominant pathogen in both blood samples and ticks of pet dogs, with the detection rates of 16.29 and 16.74%, respectively. Moreover, 17 ticks and 1 blood sample were co-infected with two pathogens, and 1 tick was co-infected with three pathogens. This study provided molecular evidence for the occurrence of Anaplasma spp., Ehrlichia spp., Rickettsia spp., and Brucella spp. circulating in pet dogs and their parasitic ticks in Junggar Basin, north-western China. These findings extend our knowledge of the tick-borne pathogens in pet dogs and their parasitic ticks in Central Asia; therefore, further research on these pathogens and their role in human and animal diseases is required.

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Molecular detection and phylogenetic analysis of Ehrlichia canis in a Philippine dog

Cited by 5 publications

References 14 publications

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

Detection and Laboratory Findings Due to Ehrlichia Canis in Dogs From the South Area of São Paulo – Sp, Brazil

Molecular Detection of Zoonotic and Veterinary Pathogenic Bacteria in Pet Dogs and Their Parasitizing Ticks in Junggar Basin, North-Western China

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