Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran

Yousefi, Arman; Rahbari, S; Shayan, Parviz; Dehkordi, Zainab Sadeghi; Bahonar, Alirezaا

doi:10.1016/j.apjtb.2017.01.017

Cited by 55 publications

(50 citation statements)

References 11 publications

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“…Moreover, sheep housing and management is comparatively poor in rural areas, hence sheep is more susceptible to tick infestation due to rough wool. Other studies reported [20,28], however, higher prevalence in goat than sheep. This may be due to difference in the geographical location and farming condition of the study areas.…”

Section: Resultsmentioning

confidence: 79%

First detection on prevalence of Anaplasma marginale in sheep and goat in Karak District, Pakistan

Hussain¹,

Junaid²,

Gul³

et al. 2017

APJTD

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Section: Resultsmentioning

confidence: 79%

First detection on prevalence of Anaplasma marginale in sheep and goat in Karak District, Pakistan

Hussain¹,

Junaid²,

Gul³

et al. 2017

APJTD

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“…However, direct molecular detection of A . marginale in small ruminants has been only reported in sheep from Iran [ 7 ]. R .…”

Section: Discussionmentioning

confidence: 99%

“…A . marginale infection has been previously molecularly described in sheep from Iran [ 7 ]. However, to the best of our knowledge, A .…”

Section: Introductionmentioning

confidence: 99%

First report of Anaplasma marginale infection in goats, Brazil

et al. 2018

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Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A. marginale and factors associated with the infection in goats from northeastern Brazil. A total of 403 goat blood samples were included in the study. An epidemiological questionnaire was applied to each farm owner addressing age, gender, presence of ticks and multispecies grazing. All samples were screened for A. marginale- and A. ovis-infection using primers targeting the Anaplasma spp. msp4 gene. The identity of A. marginale in the blood was confirmed by PCR amplification of msp5 followed by sequencing. Anaplasma spp. were differentiated by sequencing of the repeat region of the msp1α gene. For the statistical analysis the Chi-square or the Fisher’s exact test was used to verify association of the individual factors (age, gender, presence of ticks, and multispecies grazing) with Anaplasma spp. infection. We report the first molecular detection of A. marginale in goats from northeastern Brazil, based on msp1α, msp4 and msp5 gene sequencing analysis. Sequencing of the detected A. marginale msp1α gene revealed the F repeat. Amblyomma parvum and R. microplus were found feeding on animals.

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“…Anaplasma marginale, the causative agent of bovine anaplasmosis, has been detected in goats from the northeastern region of Brazil (Da Silva et al, 2018) and sheep from Iran (Yousefi et al, 2017). However, the epidemiological and clinical effects of A. marginale infection in small ruminants remain to be fully established.…”

Section: Introductionmentioning

confidence: 99%

Survey of vector-borne and nematode parasites involved in the etiology of anemic syndrome in sheep from Southern Brazil

Mongruel

Spanhol

Valente

et al. 2020

Rev. Bras. Parasitol. Vet.

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Although anemia has been historically linked to Haemonchus contortus infection, other infectious agents, such as hemotropic mycoplasmas and tick-borne disease pathogens, may also lead to anemic crisis in sheep. This study has aimed to investigate infections related to anemia in a sheep herd from Bandeirantes City, Paraná State, southern Brazil. Seven out of forty-two (16.6%; 95% CI: 8.32–30.6%) sheep were positive for hemoplasmas by a PCR targeting the 16S rRNA gene and all tested negative for A. marginale/A. ovis and Babesia/Theileria spp. by PCR based on msp4 and 18S rRNA genes, respectively. Two (4.7%; 95% CI: 1.32–15.79%) animals were infested with Rhipicephalus microplus ticks. Fecal egg counting was performed in 38 sheep and 24 (63.15%; 95% CI: 47.2–76.6%) presented > 500 eggs per gram. Phylogenetic analysis of partial sequences of the detected hemotropic Mycoplasma sp. 16S and 23S rRNA genes confirmed that the animals were infected with Mycoplasma ovis. Polymorphism analysis of partial 16S rRNA sequences showed three different genotypes of M. ovis infecting sheep assessed in the present study. Mycoplasma ovis and gastrointestinal nematodes occurs in sheep from the northern region of Paraná State.

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Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran

Cited by 55 publications

References 11 publications

First detection on prevalence of Anaplasma marginale in sheep and goat in Karak District, Pakistan

First detection on prevalence of Anaplasma marginale in sheep and goat in Karak District, Pakistan

First report of Anaplasma marginale infection in goats, Brazil

Survey of vector-borne and nematode parasites involved in the etiology of anemic syndrome in sheep from Southern Brazil

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