Molecular detection of CTX-M-15-type β-lactamases in Escherichia coli strains from Senegal

Dia, Mamadou Lamine; Ngom, Babacar; Diagne, Rokhaya; Ka, R.; Lo, Seynabou; Cissé, Moussa; Arlet, Guillaume; A, Sow

doi:10.1016/j.nmni.2015.11.009

Cited by 12 publications

(14 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The worldwide expansion of the community acquired ESBL gene, CTX-M [39] is thus confirmed in this study and also its association with TEM and SHV genes, previously reported in studies on E. coli from urinary specimens in Europe [40], Asia [41,42], America [43,44] [25]. The combination of ESBL genes is more common in this study 72/91, 79.12% than in Burkina Faso, with 6/96, 6.96% [24], in Senegal, 13/32, 40.63% [45] and in Morocco, 2/7, 28.57% [35].…”

Section: Discussionsupporting

confidence: 89%

First Detection of Resistance Genes Encoding Extended Spectrum Beta-Lactamase Producing Escherichia coli at Lome, Togo

Hr¹,

Deshmukh²,

Shivanna³

et al. 2016

Arch Clin Microbiol

View full text Add to dashboard Cite

Background: Extended Spectrum β-lactamases (ESBLs) are enzymes produced by some bacteria that inactivate oxyimino-cephalosporins, a class of β-lactam antibiotics family. They are mainly encoded by the β-lactamase gene families TEM, SHV and CTX-M. The Enterobacteriaceae expressing these genes, in particular Escherichia coli are widely expanded in the world with different prevalence rate from one region to another. The objective of this study, carried out from strains isolated at the National Institute of Hygiene (INH) in Togo, was to (i) detect and characterize the genes involving in the production of β-lactamase among E. coli strains and (ii) evaluate their prevalence.Methodology: From May 2013 to July 2015, 91 E. coli strains resistant to third generation cephalosporins (C3G) were collected from several specimens and analyzed for the detection of β-lactamase resistance genes, bla TEM , bla SHV and bla CTX-M by simplex and multiplex PCR.Results: The E. coli strains resistant at least to one third generation cephalosporin, were isolated from urine 58/91 (63.74%), vaginal samples 17/91 (17.68%), wound swabs 7/91 (7.69%), 4/91 semen samples (4.40%), urethral curettage CU 2/91 (2.20%), sputum 1/91 (1.1%), stool 1/91 (1.1%) and joint fluid 1/91 (1.1%). Resistance to third generation cephalosporins was associated with a resistance to quinolone (96.67%), trimethoprim/sulfamethoxazole (94.44%), gentamicin (75.82%) and chloramphenicol (59.09%). ESBL genes were present in all E. coli strains with the following proportions: TEM/CTX-M1 52/91 (57.14%), TEM/SHV/CTX-M1 19/91 (20.88%), CTX-M 18/91 (19.78%), TEM 1/91 (1.10%) and TEM/SHV 1/91 (1.10%). Conclusion:The presence of ESBL producing E. coli is a reality in Togo and their wide dissemination compromises the treatment of classical infections such as urinary tract infection, bacterial pneumonia, sepsis or meningitis. The acquisition of molecular data on bacterial resistance to antibiotics is necessary for better therapeutic management of these infections. This is a first determination of the βlactamase genes in Togo.

show abstract

Section: Discussionsupporting

confidence: 89%

First Detection of Resistance Genes Encoding Extended Spectrum Beta-Lactamase Producing Escherichia coli at Lome, Togo

Hr¹,

Deshmukh²,

Shivanna³

et al. 2016

Arch Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…However, molecular methods such as PCR are not readily available in the hospital setting and can only be seen in research facilities. A study by Dia et al (2015) identified 32 E. coli isolates to be ESBLs producers and blaCTX-M gene was reported to be the most frequent ESBL gene with 90.63% frequency. Similarly, Ugbo et al (2020) and Sharma et al (2010) reported 55 and 56% blaTEM frequency, respectively among E. coli of clinical origin; and this is in agreement with this present study where we reported 32.7 and 67.3% frequencies for TEM and CTX-M genes, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Microbial resistance by pathogenic Escherichia coli is a major worldwide concern. Antibacterial agents, especially beta-lactams are becoming less useful against Enterobacteriaceae (Dia et al, 2015). Urinary tract infections (UTIs), a common nosocomial and communityacquired bacterial infection, occur in all genders and age groups (Abdulaziz et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Isolation and molecular characteristics of extended spectrum beta-lactamase-producing uropathogenic Escherichia coli isolated from hospital attendees in Ebonyi State, Abakaliki

Ugbo¹,

Anyamene²,

Moses³

et al. 2020

Afr. J. Biotechnol.

View full text Add to dashboard Cite

“…Illustrative map of Europe showing countries with description of E-ESBL in cattle and beta-lactamases type.2014;Dia et al, 2016;Fam et al, 2011;Hasan et al, 2016;Hern andez et al, 2012;Liao et al, 2017;Poirel et al, 2013;Ruiz et al, 2011;Sidjabat et al, 2010).…”

mentioning

confidence: 99%

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

View full text Add to dashboard Cite

Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

show abstract

Molecular detection of CTX-M-15-type β-lactamases in Escherichia coli strains from Senegal

Cited by 12 publications

References 11 publications

First Detection of Resistance Genes Encoding Extended Spectrum Beta-Lactamase Producing Escherichia coli at Lome, Togo

First Detection of Resistance Genes Encoding Extended Spectrum Beta-Lactamase Producing Escherichia coli at Lome, Togo

Isolation and molecular characteristics of extended spectrum beta-lactamase-producing uropathogenic Escherichia coli isolated from hospital attendees in Ebonyi State, Abakaliki

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Contact Info

Product

Resources

About