Molecular detection of integron and antimicrobial resistance genes in multidrug resistant Salmonella isolated from poultry, calves and human in Beni-Suef governorate, Egypt

Aziz, Sahar Abdel; Abdel-Latef, Gihan K.; Shany, Salama A. S.; Rouby, Sherin

doi:10.1016/j.bjbas.2018.06.005

Cited by 18 publications

(12 citation statements)

References 53 publications

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“…Meanwhile, all human isolates included bla TEM , int 1 and int 3 genes (100%). These results agreed with those obtained by Abdel Aziz et al [ 42 ], who detected bla TEM and int 1 in all the tested Salmonella serovars recovered from broilers and humans. Additonally, the obtained results supported the fact that TEM-1 is the most popular plasmid mediated β-Lactamase in resistant Gram-negative bacteria [ 43 ].…”

Section: Discussionsupporting

confidence: 93%

“…Integrons are genetic elements that can recognize and capture movable gene cassettes carrying the AMR genes leading to MDR dissemination and limiting the chemotherapeutic options available for infectious diseases [ 42 ]. Regarding integrons genes, our results supported that int 1 (class 1 integrons) was the most frequent in Salmonellae [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

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Genetic Correlation of Virulent Salmonella Serovars (Extended Spectrum β-Lactamases) Isolated from Broiler Chickens and Human: A Public Health Concern

et al. 2022

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This study aimed to detect the virulent Salmonella serovars (including ESBLs producing) isolated from broiler chickens and humans. Three hundred broilers and sixty human fecal samples were bacteriologically examined. Thirty (10%) and fourteen (23.4%) Salmonella isolates were recovered from broiler and human samples, respectively. The most predominant serovar was S. enteritidis and S. typhimurium. All Salmonella isolates were confirmed by conventional PCR-based invA and ompA genes. Multidrug resistant (MDR) isolates were screened for the detection of adrA and csgD biofilm-associated genes, which were found in all isolated serovars except one S. typhimurium and 2 S. infantis of chicken isolates that were devoid of the adrA gene. Moreover, MDR isolates were screened for detection of seven resistance genes including ESBLs and other classes of resistance genes. Chicken isolates harbored blaTEM, int1, blaCTX and qnrS genes as 100, 27.8, 11.1 and 11.1%, respectively, while all human isolates harbored blaTEM, int1 and int3 genes. The genetic correlations between virulent Salmonella serovars (including antimicrobial resistance) avian and human origins were compared. In conclusion, the high prevalence of virulent ESBL producing Salmonella serovars in broilers and humans with genetic correlations between them might be zoonotic and public health hazards.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Genetic Correlation of Virulent Salmonella Serovars (Extended Spectrum β-Lactamases) Isolated from Broiler Chickens and Human: A Public Health Concern

et al. 2022

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“…Besides the datasets from the publications included in this review and meta-analysis, other relevant information was available in new articles that were published in the years 2017 and 2018 while the manuscript was under preparation by the authors. During this period, 6 full articles and three published abstracts representing 11 datasets were retrieved using the search engines (67–75). The majority of these studies were reported from Africa (67, 68, 70–74) except for two studies from Europe (69) and South America (75).…”

Section: Discussionmentioning

confidence: 99%

“…During this period, 6 full articles and three published abstracts representing 11 datasets were retrieved using the search engines (67–75). The majority of these studies were reported from Africa (67, 68, 70–74) except for two studies from Europe (69) and South America (75). Among the total of 5,868 cattle examined, 9.2% (554/6018), which is nearly equal to the pooled prevalence estimate, were reported to be positive for Salmonella species with different serotypes.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Serotype Diversity of Salmonella in Apparently Healthy Cattle: Systematic Review and Meta-Analysis of Published Studies, 2000–2017

et al. 2019

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Salmonellosis is a leading cause of foodborne illnesses in humans with cattle being one of the reservoirs for Salmonella . We estimated a pooled prevalence of Salmonella in apparently healthy cattle and examined serotype diversity through systematic review and meta-analysis of studies published between 2000 and 2017. Peer reviewed publications reporting the prevalence of Salmonella in cattle were searched through five electronic databases (PubMed, Google scholar, Agricola, Scopus, CAB direct) and through manual search. We obtained 71 publications with 75 datasets consisting a total of 52,766 animals examined and 5,010 Salmonella positive cattle from 29 countries in six continents (except from Antarctica). Pooled prevalence of Salmonella in cattle was 9% (95% confidence interval: 7–11%). Significantly high heterogeneity ( I 2 = 98.7%, P < 0.01) was observed among all studies as well as within continents. Prevalence varied from 2% (Europe) to 16% (North America). Overall, 143 different serotypes were reported with the most diverse serotypes being reported from Africa (76 different serotypes) followed by North America (49 serotypes). The 10 most frequently reported serotypes (Montevideo, Typhimurium, Kentucky, Meleagridis, Anatum, Cerro, Mbandaka, Muenster, Newport, and Senftenberg) accounted for 65% of the isolates for which specific serotype information was reported. Salmonella Montevideo and S . Dublin are the most frequently reported serotypes in North America and Europe, respectively, while S . Typhimurium was the most frequent in Africa, Asia and Australasia. Our results indicated variability both in the prevalence and serotype diversity of Salmonella in cattle across continents. Although all Salmonella serotypes are potentially pathogenic to humans, five (Montevideo, Typhimurium, Anatum, Mbandaka, and Newport) of the top 10 serotypes identified in this study are among the serotypes most commonly associated with clinical illnesses in humans.

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“…The inconsistency between some resistance genes and resistance phenotypes observed in this study indicates that the resistance was not borne by the genes tested. These may have been encoded by mobile genetic elements such as integrase/gene cassette, transposons and plasmids, or other resistance genes as earlier suggested by Gharieb et al (2015), Partridge et al (2018), Abdel Aziz et al (2018), Zhang et al 2018; McMillan et al 2019; Chen et al 2020) and these deserve further investigation. Antimicrobial resistance originated from antibiotics use in livestock production, disease prevention and growth‐promoting feed additives, as well as from indiscriminate use, ultimately leading to deposits of drug residues in the animal’s tissues and the environment (Sattar et al 2014).…”

Section: Discussionmentioning

confidence: 89%

Antimicrobial resistance and virulence genes inSalmonella entericaserovars isolated from droppings of layer chicken in two farms in Nigeria

Shittu

Uzairue

Ojo

et al. 2022

Journal of Applied Microbiology

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Aim This study aimed to investigate the isolation rate, antibiotic resistance and virulence genes of Salmonella enterica serovar from two commercial farms in Nigeria. Methods and Results Salmonella isolation was performed according to the United States Food and Drug Agency (USFDA) method. Serotyping, antimicrobial susceptibility testing, detection of resistance and virulence genes were done using the Kauffman–White Scheme, disc diffusion, minimum inhibitory concentration and real‐time polymerase chain reaction techniques. Salmonella serovars were isolated from only farm A at 22/50 (44.0%) while none were isolated from farm B. Salmonella Typhi, 9 (40.9%); Salmonella Typhimurium, 2 (9.1%), Salmonella Enteritidis, 2 (9.1%), Salmonella Pullorum, 1 (4.5%), Salmonella Kentucky, 4 (18.2%) were identified while 4 (18.2%) were untypable. Sixteen isolates (72.7%) showed multiple drug resistance and 17 different resistance profile types with AMP‐CHL‐TRM‐SXT as the most prevalent pattern. Resistance genes (blaTEM, 12/22 (54.5%) and virulence genes (InvA, sopB, mgtC and spi4D, 22/22 (100.0%), ssaQ, 16/22 (72.7%) and spvC, 13/22 (59.1%) were found, while blaSHV, blaCTX‐M, floR, tetA, tetB, tetG and LJSGI‐1 genes were absent. Conclusion Pathogenic Salmonella were isolated from the chicken droppings in this study. Most of these strains were resistant to antibiotics and possessed characteristics of virulence. Significance and Impact of the Study Chicken droppings from this study area contained pathogenic strains of Salmonella and a rare occurrence of Salmonella Typhi. The study revealed that the environment and the food chain could be at risk of contamination of highly virulent and antimicrobial‐resistant strains of Salmonella. These could affect the profitability of the poultry industry and food consumption. There is a need for caution in indiscriminate disposal of poultry waste and the use of uncomposted chicken droppings in soil amendment.

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Molecular detection of integron and antimicrobial resistance genes in multidrug resistant Salmonella isolated from poultry, calves and human in Beni-Suef governorate, Egypt

Cited by 18 publications

References 53 publications

Genetic Correlation of Virulent Salmonella Serovars (Extended Spectrum β-Lactamases) Isolated from Broiler Chickens and Human: A Public Health Concern

Genetic Correlation of Virulent Salmonella Serovars (Extended Spectrum β-Lactamases) Isolated from Broiler Chickens and Human: A Public Health Concern

Prevalence and Serotype Diversity of Salmonella in Apparently Healthy Cattle: Systematic Review and Meta-Analysis of Published Studies, 2000–2017

Antimicrobial resistance and virulence genes inSalmonella entericaserovars isolated from droppings of layer chicken in two farms in Nigeria

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