Molecular Detection of Macrolide and Lincosamide-Resistance Genes in Clinical Methicillin-Resistant Staphylococcus aureus Isolates from Kerman, Iran

Fasihi, Yasser; Saffari, Fereshteh; Ghahraman, Mohammad Reza Kandehkar; Kalantar-Neyestanaki, Davood

doi:10.5812/pedinfect.37761

Cited by 18 publications

(20 citation statements)

References 17 publications

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“…Furthermore, the erm and msr genes have been reported in Denmark, the United Kingdom, and Tunisia. In Tunisia and Denmark, ermB and ermA genes were the most common clindamycin-and erythromycin-resistant genes, respectively, but in this study, ermC was the most common [28]. In this study, according to the results of PCR, the prevalence of ermC gene was lower than that of the ermA and msrA genes, whereas most studies report ermC as the most frequent genetic determinant [30,31].…”

Section: Discussioncontrasting

confidence: 62%

“…In addition, none of the MRSA isolates had the ermB gene that was in line with the report by Lina et al [27]. These findings were in disagreement with the study by Fasihi et al [28] performed in Kerman, Iran, in which an incidence of 11% and 3.5% was reported for ermA and ermB genes, respectively. It has been reported that the prevalence of the ermB in staphylococci isolated from animal sources is higher than those isolated from human specimens [29].…”

Section: Discussionsupporting

confidence: 49%

“…The dissimilarities in the prevalence rate of MLSB resistance genes in different studies may be explained by the heterogeneous nature of erythromycin resistance, or it may be due to the loss of small plasmids that carry erm and msr genes [28]. We identified the ermA + msrA + ermC gene combinations in 28.3% of the MRSA isolates.…”

Section: Discussionmentioning

confidence: 87%

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Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among methicillin-resistant Staphylococcus aureus strains isolated from burn patients

Khoshnood

Shahi

Jomehzadeh³

et al. 2019

AMicr

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The increasing resistance to macrolide, lincosamide, and streptogramin B agents among methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide problem for the health community. This study aimed to investigate the prevalence of ermA, ermB, ermC, and msrA in MRSA strains isolated from burn patients in Ahvaz, southwest of Iran. A total of 76 isolates of S. aureus were collected from January to May 2017 from Taleghani Burn Hospital in Ahvaz. Among 76 S. aureus strains collected, 60 (78.9%) isolates were MRSA. The antimicrobial susceptibility testing for MRSA showed extreme high resistance rate to clarithromycin (100%) and azithromycin (100%), followed by erythromycin (98.3%). The PCR assay revealed that the frequency rates of msrA, ermA, and ermC genes were 23 (38.3%), 28 (46.7%), and 22 (36.7%), respectively. In addition, none of the MRSA isolates had the ermB gene. Because of the high prevalence of macrolide and lincosamide resistance found in MRSA isolates from infections of burn patients in Ahvaz, southwest of Iran, it is recommended that local periodic survey be performed for controlling the dissemination of antimicrobial resistance.

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Section: Discussioncontrasting

confidence: 62%

Section: Discussionsupporting

confidence: 49%

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Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among methicillin-resistant Staphylococcus aureus strains isolated from burn patients

Khoshnood

Shahi

Jomehzadeh³

et al. 2019

AMicr

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“…17 These findings are in concordance with those described by Fasihi et al, which reported prevalence of erm(C) and erm(A) genes to be 20.5% and 11% for MRSA strains. 35 In accordance with our results, Schmitz and colleagues reported that the erm(A) gene was more common in MRSA isolates compared to MSSA isolates (88% vs 38%) and occurred mainly in cMLS B expression strains. 36 A high percentage of msr(A) (20.9%) was obtained in the current research, similar to a study conducted by Sedaghat et al (43.6%), 17 Nezhad et al (47.3%).…”

Section: Discussionsupporting

confidence: 92%

<p>Molecular Characterization, Drug Resistance and Virulence Analysis of Constitutive and Inducible Clindamycin Resistance <em>Staphylococcus aureus</em> Strains Recovered from Clinical Samples, Tehran – Iran</p>

Goudarzi¹,

Tayebi²,

Fazeli³

et al. 2020

IDR

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Background: Macrolide-lincosamide streptogramin B family is one of the important alternative antibiotics for treating staphylococcal infections. The aim of this study was to determine the characteristics and prevalence of antibiotic resistance genes in different coagulase types of clinical Staphylococcus aureus strains. Methods: In the present study, 86 isolates with different phenotypes of MLS B resistance were investigated. In vitro susceptibility was assessed by the disk diffusion and broth microdilution methods. PCR assays were used to detect resistance-related genes. Coagulase and SCCmec types were identified by multiplex PCR assay. Results: The prevalences of constitutive MLS B , inducible MLS B , and MS phenotypes were found to be 23%, 14.2%, and 4.9%, respectively. The rates of resistance to mupirocin, fusidic acid, and tigecycline were found to be 9.3%, 4.6%, and 2.3%, respectively. The top three predominant resistance genes were mecA, tet(M), erm(C) representing 75.6, 50, and 40.7% of isolates. mupA (7%), fusB (3.5%), and fusC (1.2%) genes were also detected among tested isolates. Coagulase types were mainly type II (34.9%), followed by III (32.6%), V (20.9%), and I (11.6%). Conclusion: These findings indicated high resistance rate and low genetic variability with the prominence of coa type II, highlighting the particular importance of diagnosis of these strains to avoid treatment failure.

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“…In the antibiotic sensitivity test, higher number of isolates were resistant towards penicillin (100 %) followed by erythromycin ( (Havaei et al, 2016). Several other studies (Aydeniz et al, 2015;Fasihi et al, 2016) including our study was in between this range of prevalence rate.…”

Section: Discussionsupporting

confidence: 55%

Screening of erm Gene of Inducible Clindamycin Resistant Staphylococcus aureus

Timsina¹,

Timalsina

Singh

2019

J. Inst. Sci. Tech.

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Antibiotic resistance exhibited by Staphylococcus aureus is a growing global concern. This work was undertaken to determine the prevalence rate of inducible clindamycin resistant S. aureus in nasal sample and detect ermB gene in the isolates with inducible clindamycin resistance. Nasal swabs were collected from the school children and cultured on Mannitol Salt Agar (MSA) and Blood Agar (BA) for observation of colony morphology. Gram staining and biochemical test (catalase, oxidase, O-F and coagulase) were performed for further identification of the bacteria. The Kirby-Bauer disc diffusion method using a cefoxitin disc (30 μg) was used to detect methicillin resistant S. aureus (MRSA). All, the MRSA isolates were tested for ermB gene by PCR amplification. Among 64 S. aureus isolates, 17 (26 %) were MRSA. The prevalence of Inducible clindamycin resistant S. aureus (iMLSB) isolates was 23.4 % in the S. aureus isolates. All the isolates of MRSA were resistant to penicillin, while 88.2 % were sensitive to gentamicin. The prevalence of ermB gene was 3.1 % in the total S. aureus isolates and 11.7 % MRSA showed the presence of this gene. Routinely performing a D-test in laboratory will guide the clinicians on the rationale use of clindamycin and improving hygienic practices can reduce the spread of inducible clindamycin resistance.

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Molecular Detection of Macrolide and Lincosamide-Resistance Genes in Clinical Methicillin-Resistant Staphylococcus aureus Isolates from Kerman, Iran

Cited by 18 publications

References 17 publications

Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among methicillin-resistant Staphylococcus aureus strains isolated from burn patients

Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among methicillin-resistant Staphylococcus aureus strains isolated from burn patients

<p>Molecular Characterization, Drug Resistance and Virulence Analysis of Constitutive and Inducible Clindamycin Resistance <em>Staphylococcus aureus</em> Strains Recovered from Clinical Samples, Tehran – Iran</p>

Screening of erm Gene of Inducible Clindamycin Resistant Staphylococcus aureus

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