Molecular detection of novel circoviruses in ticks in northeastern China

Wang, Bo; Sun, Lidan; Liu, Huan-Huan; Wang, Zedong; Zhao, Yongkun; Wang, Wei; Liu, Quan

doi:10.1016/j.ttbdis.2018.03.017

Cited by 16 publications

(20 citation statements)

References 18 publications

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“…Ticks transmit more pathogen species than any other group of blood‐feeding arthropods worldwide, including several viral ones (Pfäffle, Littwin, Muders, & Petney, ). Tick‐infecting circoviruses have been described in China (Wang et al., ), and other arthropod species have been proven to be susceptible to Circoviridae infection (Delwart & Li, ). Nevertheless, the role of hematophagous arthropods in PCV‐3 epidemiology has never been investigated.…”

Section: Introductionmentioning

confidence: 99%

A wild circulation: High presence of Porcine circovirus 3 in different mammalian wild hosts and ticks

Franzo

Grassi

Tucciarone

et al. 2019

Transbound Emerg Dis

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Porcine circovirus 3 (PCV-3) has emerged as a potential threat for swine industry, being consistently reported in the presence of several clinical signs all around the world.Recently, its presence in wild boar has been demonstrated at high prevalence. This evidence is surprising since the lower density of wild populations might not be expected to sustain such efficient viral transmission. Porcine circoviruses were proven to exhibit a certain plasticity in the host tropism and were detected in unrelated species, like mice, dogs and ruminants. However, if this scenario applies also to wild animals remains to be established. Therefore, this study aimed to investigate the presence of PCV-3 in wild ungulates other than wild boar and in related hematophagous ectoparasites. One hundred and nine animals were sampled from different hilly and mountain areas of Friuli Venezia Giulia, including 9 chamois (Rupicapra rupicapra), 17 red deer (Cervus elaphus), 4 mouflons (Ovis musimon), 50 roe deer (Capreolus capreolus) and 29 wild boars (Sus scrofa). Additionally, host-matched ectoparasites were collected when present. Porcine circovirus 3 was diagnosed using molecular techniques and sequencing. This study results confirmed the high PCV-3 occurrence in wild boar and reported for the first time its presence, at low prevalence, in chamois and roe deer. Moreover, two ticks (Ixodes ricinus), one of which non-engorged, collected from PCV-3 negative roe deer, tested PCV-3 positive. The genetic characterization of some of the strains collected from non-swine hosts allowed to prove that, albeit clearly part of PCV-3 species, they were genetically unique, demonstrating the absence of among-samples contamination and thus confirming the actual presence of PCV-3 genome in these new hosts. Therefore, this study highlights an unexpected broad PCV-3 distribution and circulation in the wild, rising further questions on porcine circoviruses infectious cycle, epidemiology and origin, which will deserve additional investigations.

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Section: Introductionmentioning

confidence: 99%

A wild circulation: High presence of Porcine circovirus 3 in different mammalian wild hosts and ticks

Franzo

Grassi

Tucciarone

et al. 2019

Transbound Emerg Dis

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“…Most viral discovery studies focus on vertebrate hosts, primarily mammals, which limits our perspective of viral diversity and evolution. The work presented here expands on studies investigating CRESS DNA viruses in invertebrates, which have traditionally been undersampled (Dayaram et al 2013;Kraberger et al 2018;Rosario, Schenck, et al 2015;Bistolas et al 2017;Hewson, Ng, et al 2013;Wang et al 2018;Bettarel et al 2018). We report 44 CRESS DNA genomes recovered from arthropods representing all three major terrestrial arthropod lineages (Giribet and Edgecombe 2012).…”

Section: Introductionmentioning

confidence: 98%

Virus discovery in all three major lineages of terrestrial arthropods highlights the diversity of single-stranded DNA viruses associated with invertebrates

Rosario

Mettel

Benner

et al. 2018

Preprint

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Viruses encoding a replication-associated protein (Rep) within a covalently closed, single-stranded (ss)DNA genome are among the smallest viruses known to infect eukaryotic organisms, including economically valuable agricultural crops and livestock. Although circular Rep-encoding ssDNA (CRESS DNA) viruses are a widespread group for which our knowledge is rapidly expanding, biased sampling towards vertebrates and land plants has limited our understanding of their diversity and evolution. Here we screened terrestrial arthropods for CRESS DNA viruses and report the identification of 44 viral genomes and replicons associated with specimens representing all three major terrestrial arthropod lineages, namely Euchelicerata (spiders), Hexapoda (insects), and Myriapoda (millipedes). We identified virus genomes belonging to three established CRESS DNA viral families (Circoviridae, Genomoviridae, and Smacoviridae); however, over half of the arthropod-associated virus genomes are only distantly related to currently classified CRESS DNA viral sequences. Although members of viral and satellite families known to infect plants (Geminiviridae, Nanoviridae, Alphasatellitidae) were not identified in this study, these plant-infecting CRESS DNA viruses and replicons are transmitted by hemipterans. Therefore, members from six out of the seven established CRESS DNA viral families circulate among arthropods. Furthermore, a phylogenetic analysis of Reps, including endogenous viral sequences, reported to date from a wide array of organisms revealed that most of the known CRESS DNA viral diversity circulates among invertebrates. Our results highlight the vast and unexplored diversity of CRESS DNA viruses among invertebrates and parallel findings from RNA viral discovery efforts in undersampled taxa.

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“…Members of the genus Circovirus have marked genetic diversity and a broad host range, and these viruses have been identified in vertebrates and invertebrates, including birds, fish, pigs, bats, minks, dogs, foxes, chimpanzees, humans, palm civets, cats and ticks (Nishizawa et al, ; Takano, Yanai, Hiramatsu, Doki, & Hohdatsu, ; Wang et al, ). Although the pathogenicity of most circoviruses remains unknown, some circoviruses have been identified as causative agents of animal diseases.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of chicken circovirus from commercial broiler chickens in China

Yuan

Yan

et al. 2019

Transbound Emerg Dis

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Circoviruses are found in many species, including mammals, birds, lower vertebrates and invertebrates. To date, there are no reports of circovirus‐induced diseases in chickens. In this study, we identified a new strain of chicken circovirus (CCV) by PacBio third‐generation sequencing samples from chickens with acute gastroenteritis in a Shandong commercial broiler farm in China. The complete genome of CCV was verified by inverse PCR. Genomic analysis revealed that CCV codes two inverse open reading frames (ORFs), and a potential stem‐loop structure was present at the 5′ end with a structure typical of a circular virus. Phylogenetic tree analysis showed that CCV formed an independent branch between mammalian and avian circovirus, and homology analysis indicated that the homology of CCV with 21 other known circoviruses was less than 40%. Thus, this CCV strain represents a new species in the genus Circovirus. The infection rate of CCV in 12 chickens with diarrhoea was 100%, but no CCV was found in healthy chickens, thereby indicating that the novel CCV strain is highly associated with acute infectious gastroenteritis in chickens. The emergence of a novel CCV in commercial broiler chickens is highly concerning for the broiler industry.

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Molecular detection of novel circoviruses in ticks in northeastern China

Cited by 16 publications

References 18 publications

A wild circulation: High presence of Porcine circovirus 3 in different mammalian wild hosts and ticks

A wild circulation: High presence of Porcine circovirus 3 in different mammalian wild hosts and ticks

Virus discovery in all three major lineages of terrestrial arthropods highlights the diversity of single-stranded DNA viruses associated with invertebrates

Identification and characterization of chicken circovirus from commercial broiler chickens in China

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