Molecular diagnosis and analysis of Chikungunya virus

Edwards, C. R. W.; Welch, Stephen R.; Chamberlain, John; Hewson, Roger; Tolley, H. Dennis; Cane, Patricia A.; Lloyd, Graham

doi:10.1016/j.jcv.2007.05.008

Cited by 119 publications

(94 citation statements)

References 14 publications

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“…Current laboratory diagnosis of CHIK infection is based on isolation of the virus and serological and molecular methods. Reverse transcriptase PCR (RT- PCR) is a confirmatory method used to identify CHIKV (Carletti et al, 2007;Edwards et al, 2007), and while this test exhibits high specificity, it requires expensive equipment and skilled scientists to perform the test. Serological diagnostic methods such as the hemagglutination inhibition test (HI test) and ELISA have also been used to diagnose CHIKV disease.…”

Section: Evaluation Of the Recombinant Chikv Capsid Protein Using Icamentioning

confidence: 99%

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Cho¹,

Kim²,

Cho

et al. 2008

Journal of Virological Methods

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Section: Evaluation Of the Recombinant Chikv Capsid Protein Using Icamentioning

confidence: 99%

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Cho¹,

Kim²,

Cho

et al. 2008

Journal of Virological Methods

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“…The molecular diagnosis of CHIKV has only been evaluated for human samples [16][17][18][19][20][21][22][23] and no real-time RT-PCR assay exists for detecting ONNV present in human or mosquito F igure 1. Detection of CHIKV in infected mosquitoes using real-time reverse transcription-polymerase chain reaction.…”

Section: Discussionmentioning

confidence: 99%

“…Several groups have developed real-time RT-PCR assays for the molecular diagnosis of CHIK involving human samples. [16][17][18][19][20][21][22][23] However, these assays have not been evaluated for detecting CHIKV RNA in infected mosquitoes. Additionally, no real-time RT-PCR assay exists for detecting ONNV RNA in human or mosquito samples.…”

Section: Introductionmentioning

confidence: 99%

Development of Field-Based Real-Time Reverse Transcription–Polymerase Chain Reaction Assays for Detection of Chikungunya and O’nyong-nyong Viruses in Mosquitoes

et al. 2009

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Abstract. Chikungunya (CHIK) and O'nyong-nyong (ONN) are important emerging arthropod-borne diseases. Molecular diagnosis of these two viruses in mosquitoes has not been evaluated, and the effects of extraneous mosquito tissue on assay performance have not been tested. Additionally, no real-time reverse transcription-polymerase chain reaction (RT-PCR) assay exists for detecting ONN virus (ONNV) RNA. We describe the development of sensitive and specific real-time RT-PCR assays for detecting CHIK and ONN viral RNA in mosquitoes, which have application for field use. In addition, we compared three methods for primer/probe design for assay development by evaluating their sensitivity and specificity. This comparison resulted in development of virus-specific assays that could detect less than one plaqueforming unit equivalent of each of the viruses in mosquitoes. The use of these assays will aid in arthropod-borne disease surveillance and in the control of the associated diseases.

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“…The sensitivity and specificity of genetic methods developed for CHIKV diagnosis can reach 100%. Moreover, real-time RT-PCR combined with sequence analysis was used to identify CHIKV genotypes, while multiplex PCR were utilized to detect CHIKV and dengue infection simultaneously in one tube (Edwards et al, 2007;Cecilia et al, 2015). Although detection of viral RNA is most useful for rapid identification of the infectious virus, it is still limited by the narrow detection window of first week of illness.…”

Section: Genetic Methodsmentioning

confidence: 99%