Molecular diversity and phylogeny of Triticum-Aegilops species possessing D genome revealed by SSR and ISSR markers

Abstract

The aim of this study is investigation the applicability of SSR and ISSR markers in evaluating the genetic relationships in twenty accessions ofAegilopsandTriticumspecies with D genome in different ploidy levels. Totally, 119 bands and 46 alleles were detected using ten primers for ISSR and SSR markers, respectively. Polymorphism Information Content values for all primers ranged from 0.345 to 0.375 with an average of 0.367 for SSR, and varied from 0.29 to 0.44 with the average 0.37 for ISSR marker. Analysis of molecular variance (AMOVA) revealed that 81% (ISSR) and 84% (SSR) of variability was partitioned among individuals within populations. Comparing the genetic diversity ofAegilopsandTriticumaccessions, based on genetic parameters, shows that genetic variation ofAe. crassaandAe. tauschiispecies are higher than other species, especially in terms of Nei’s gene diversity. Cluster analysis, based on both markers, separated total accessions in three groups. However, classification based on SSR marker data was not conformed to classification according to ISSR marker data. Principal co-ordinate analysis (PCoA) for SSR and ISSR data showed that, the first two components clarified 53.48% and 49.91% of the total variation, respectively. This analysis (PCoA), also, indicated consistent patterns of genetic relationships for ISSR data sets, however, the grouping of accessions was not completely accorded to their own geographical origins. Consequently, a high level of genetic diversity was revealed from the accessions sampled from different eco-geographical regions of Iran.

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

“…All three marker systems showed the highest PPL in the northwestern genotypes, indicating a high allelic diversity and the heterogeneity of these genotypes. Our results confirmed that the main center for the diversity of the genus Aegilops in Iran was most probably located in the north and northwest of the country [44].…”

Genetic relationships and diversity among wild Salvia accessions revealed by ISSR and SCoT markers

“…DNA analysis using ISSR and SCoT markers has proved to be an efficient and inexpensive way to provide molecular data to evaluate genetic variation, and it has been used successfully to determine genetic relationships for many plants [12,14,17,18,[30][31][32][33]. In the present study, the ISSR technique revealed higher mean PB (16.67) than SCoT (16.45).…”

Assessment of genetic diversity inTriticum urartuThumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

“…Genetic diversity in the wheat germplasm opens up new scenarios to the discovery of useful genes or alleles and improvement of varieties with desirable traits, which include both farmer-and breed-preferred traits such as yield potential, large seed, high seed weight and tolerance to both biotic and abiotic stresses [21]. Previously, the genetic diversity and population structure of T. uraru populations have been studied using a large number of marker systems, including morphological characters [22], isozymes [23] and molecular markers [20,24,25].…”