Molecular Epidemiology and Association of Putative Pathogens in Root Canal Infection

Abstract

The purpose of this study was to investigate the epidemiology of putative pathogens in root canals with apical periodontitis and to determine the associations among the putative pathogens. Eighteen symptomatic and 20 asymptomatic teeth from 36 subjects were studied. This research was performed with polymerase chain reaction and hybridization using rRNA-based oligonucleotide probes. The most frequently found species was Fusobacterium sp. (68.4%), followed by Peptostreptococcus micros (44.7%) and Porphyromonas gingivalis (26.3%). Sixteen teeth (42.1%) contained one or more species of the selected black-pigmented bacteria. Bacteroides forsythus and Treponema sp. were detected in 8 teeth and 6 teeth, respectively. Among the analyzed bacteria, significant relationships were shown in the combination of B. forsythus/P. gingivalis and Treponema sp./P. gingivalis. There was no significant association between any bacteria and any symptoms.

The effect ofTreponema denticolaimmunoinhibitory protein on cytokine expression in T cells

“…The Effect of Treponema denticola immunoinhibitory protein on cytokine expression in T cells Treponema denticola have been rarely found associated with endodontic diseases, however, unidentified spirochete species have now been found in endodontic infections by employing polymerase chain reaction (PCR) method 6,[15][16][17] . In this regard, Smallwood et al 18) recently reported the occurrence of spirochetes in all samples collected from infected canals using PCR.…”

Isolation of microrganisms involved in endodontic infection

“…Siqueira, et al hanno dimostrato che T. denticola è associata alla presenza di ascessi di origine endodontica, con una positività del 79% (22). Jung, et al, invece non hanno trovato alcuna correlazione tra T. denticola e sintomi clinici (11). Studi precedenti hanno ipotizzato inoltre che T. denticola potrebbe essere coinvolto nella differenziazione degli osteoclasti durante le infezioni endodontiche, a causa dei fattori di virulenza di cui è dotata (2,5).…”

Lezyon Sterilizasyonu ve Doku Tamiri

“…Literatür bilgilerine göre, oral mikroflorada 300 ile 500 arasında bakteri türü bulunmaktadır. 2,3 Ancak, bunlardan sadece bir kısmı (150 civarında) pulpaya çeşitli yollarla (koronal, retrograd veya hematojen) girerek, pulpa hastalıklarına neden olabilmektedir. [4][5][6][7][8][9] Kök kanalındaki bu bakteriler 10-12 çeşit genustan oluşmaktadır.…”