Molecular epidemiology of Legionella pneumophila serogroup 1 isolates identify a prevalent sequence type, ST505, and a distinct clonal group of clinical isolates in Toyama Prefecture, Japan

Kanatani, Jun-ichi; Isobe, Junko; Kimata, Keiko; Shima, Tomoko; Shimizu, Miwako; Kura, Fumiaki; Sata, Tetsutaro; Watahiki, Masanori

doi:10.1007/s10156-012-0537-x

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…According to the studies of the distribution of Lp1 STs in different regions of the world, it appears that the most prevalent ST among clinical and environmental populations of Lp1 is either ST1 (8,(27)(28)(29)(30)(31)(32) or an ST that is confined to a specific geographic region (33)(34)(35). For example, in Belgium and the United Kingdom, the most frequently detected ST among clinical isolates was ST47 (9,35).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Sequence Types among Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 in the United States from 1982 to 2012

et al. 2014

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cSince the establishment of sequence-based typing as the gold standard for DNA-based typing of Legionella pneumophila, the Legionella laboratory at the Centers for Disease Control and Prevention (CDC) has conducted routine sequence-based typing (SBT) analysis of all incoming L. pneumophila serogroup 1 (Lp1) isolates to identify potential links between cases and to better understand genetic diversity and clonal expansion among L. pneumophila bacteria. Retrospective genotyping of Lp1 isolates from sporadic cases and Legionnaires' disease (LD) outbreaks deposited into the CDC reference collection since 1982 has been completed. For this study, we compared the distribution of sequence types (STs) among Lp1 isolates implicated in 26 outbreaks in the United States, 571 clinical isolates from sporadic cases of LD in the United States, and 149 environmental isolates with no known association with LD. The Lp1 isolates under study had been deposited into our collection between 1982 and 2012. We identified 17 outbreak-associated STs, 153 sporadic STs, and 49 environmental STs. We observed that Lp1 STs from outbreaks and sporadic cases are more similar to each other than either group is to environmental STs. The most frequent ST for both sporadic and environmental isolates was ST1, accounting for 25% and 49% of the total number of isolates, respectively. The STs shared by both outbreak-associated and sporadic Lp1 included ST1, ST35, ST36, ST37, and ST222. The STs most commonly found in sporadic and outbreak-associated Lp1 populations may have an increased ability to cause disease and thus may require special attention when detected.

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Section: Discussionmentioning

confidence: 99%

Prevalence of Sequence Types among Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 in the United States from 1982 to 2012

et al. 2014

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“…A total of 140 L. pneumophila SG 1 isolates were analyzed, including isolates from puddles on roads (n ϭ 62), public baths (n ϭ 51), cooling towers (n ϭ 5), showers (n ϭ 3), and sputum specimens (n ϭ 19). Among these, 62 isolates from puddles and 5 isolates from other sources (1 isolate from a cooling tower, 2 isolates from 2 showers, and 2 isolates from 2 patients) obtained in this study were collected from 2010 to 2011 and from 2010 to 2012, respectively, in Toyama Prefecture, Japan (Table 1); the remaining 73 isolates (51 isolates from 24 public baths, 4 isolates from 2 cooling towers, 1 isolate from a shower, and 17 isolates from 16 patients), obtained in a previous study, were collected from 2005 to 2012 in Toyama Prefecture, Japan (22).…”

Section: Methodsmentioning

confidence: 97%

“…We analyzed 62 L. pneumophila SG 1 isolates obtained from puddles on asphalt roads in comparison with 73 L. pneumophila SG 1 stock strains from a previous study (22) and 5 isolates obtained from sources other than puddles in this study ( Table 1). The 19 (total) clinical isolates from sputum specimens were collected from 4 hospitals in Toyama Prefecture.…”

Section: Isolation Ofmentioning

confidence: 99%

“…However, the genetic relationships between strains from clinical specimens and from these environmental sources have not been clearly analyzed by molecular typing techniques. Furthermore, our previous study reported that the comparative analysis of L. pneumophila SG 1 isolates from sputum specimens and public baths found a clonal group formed only by clinical isolates that were not associated with bath water (22). The short genetic distance between strains of the clonal group suggested that they were derived from a common and unrecognized type of source.…”

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Close Genetic Relationship between Legionella pneumophila Serogroup 1 Isolates from Sputum Specimens and Puddles on Roads, as Determined by Sequence-Based Typing

Kanatani

Isobe

Kimata

et al. 2013

Appl Environ Microbiol

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bWe investigated the prevalence of Legionella species isolated from puddles on asphalt roads. In addition, we carried out sequence-based typing (SBT) analysis on the genetic relationship between L. pneumophila serogroup 1 (SG 1) isolates from puddles and from stock strains previously obtained from sputum specimens and public baths. Sixty-nine water samples were collected from puddles on roads at 6 fixed locations. Legionella species were detected in 33 samples (47.8%) regardless of season. Among the 325 isolates from puddles, strains of L. pneumophila SG 1, a major causative agent of Legionnaires' disease, were the most frequently isolated (n ‫؍‬ 62, 19.1%). Sixty-two isolates of L. pneumophila SG 1 from puddles were classified into 36 sequence types (STs) by SBT. ST120 and ST48 were identified as major STs. Environmental ST120 strains from puddles were found for the first time in this study. Among the 14 STs of the clinical isolates (n ‫؍‬ 19), 4 STs (n ‫؍‬ 6, 31.6%), including ST120, were also detected in isolates from puddles on roads, and the sources of infection in these cases remained unclear. The lag-1 gene, a tentative marker for clinical isolates, was prevalent in puddle isolates (61.3%). Our findings suggest that puddles on asphalt roads serve as potential reservoirs for L. pneumophila in the environment.

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Prevalence of Legionella species isolated from shower water in public bath facilities in Toyama Prefecture, Japan

Kanatani

Isobe

Norimoto

et al. 2017

Journal of Infection and Chemotherapy

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Molecular epidemiology of Legionella pneumophila serogroup 1 isolates identify a prevalent sequence type, ST505, and a distinct clonal group of clinical isolates in Toyama Prefecture, Japan

Cited by 9 publications

References 27 publications

Prevalence of Sequence Types among Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 in the United States from 1982 to 2012

Prevalence of Sequence Types among Clinical and Environmental Isolates of Legionella pneumophila Serogroup 1 in the United States from 1982 to 2012

Close Genetic Relationship between Legionella pneumophila Serogroup 1 Isolates from Sputum Specimens and Puddles on Roads, as Determined by Sequence-Based Typing

Prevalence of Legionella species isolated from shower water in public bath facilities in Toyama Prefecture, Japan

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